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TMA | nmz787: Does the $1800 include the $700 CT? or is the CT extra, bringing the total to $2500? | 02:54 |
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abetusk | nmz787, did you get your brl-cad to gcode converter going? | 03:05 |
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kanzure | "Neurostimulation: Bright sparks" http://www.nature.com/nature/journal/v531/n7592_supp/full/531S6a.html | 05:21 |
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kanzure | "Repurposing the ribosome for synthetic biology | 05:25 |
kanzure | "Repurposing the ribosome for synthetic biology" http://www.nanowerk.com/nanotechnology-news/newsid=43917.php | 05:25 |
kanzure | " | 05:26 |
kanzure | "“The ability to make synthetic polymers with perfect sequence control is a holy grail in polymer science,” said Charles Schroeder, associate professor and Ray and Beverly Mentzer Scholar in Chemical and Biological Engineering at the University of Illinois at Urbana-Champaign. “Our approach holds strong promise to enable the synthesis of precision polymeric materials with levels of control that have not yet been achievable.”" | 05:26 |
kanzure | "While the ribosome already makes biopolymers, such as proteins like insulin or subtilisin in laundry detergents, the MURI team aims to teach the ribosome to make different types of polymers, enabled by chemistry that has yet to exist in the living world. Polymer scientists at the University of Illinois, including Schroeder and Jeff Moore, will then test and characterize the work, which could have applications well beyond batteries, such ... | 05:26 |
kanzure | ... as in nanoscale self-assembly, electromagnetic interference shielding, and anticorrosion coatings for steel" | 05:27 |
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kanzure | the above is somewhat re: my tRNA synthetase proposal for controlled ribosome https://groups.google.com/d/msg/enzymaticsynthesis/3YEEv0OULo0/zJZPETWDbMIJ | 05:30 |
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kanzure | some sort of indecipherable machine learning genomics thingy http://www.deepgenomics.com/ | 05:59 |
kanzure | http://www.deepgenomics.com/tech/ | 06:00 |
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maaku | https://people.csail.mit.edu/devadas/pubs/riffle.pdf | 06:38 |
maaku | .title | 06:38 |
maaku | .title http://news.mit.edu/2016/stay-anonymous-online-0711 | 06:38 |
yoleaux | How to stay anonymous online | MIT News | 06:38 |
yoleaux | maaku: Sorry, that doesn't appear to be an HTML page. | 06:38 |
maaku | Riffle - An Efficient Communication System With Strong Anonymity | 06:38 |
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kanzure | seanph: sup | 06:50 |
seanph | @kanzure hey Bryan. just doing some programming, you? | 06:50 |
cynsia | programming anything interesting? | 06:51 |
kanzure | seanph: negotiation stuff :(.... also synthetic viruses for dog cancer reasons. | 06:53 |
seanph | researching those viruses? | 06:53 |
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seanph | @cynsia https://github.com/skycoin/skycoin/tree/master/src/mesh2 | 06:54 |
kanzure | ouch | 06:55 |
seanph | @cynsia mesh network, designed with great firewall spanning in mind | 06:55 |
kanzure | it's hilarious that the bitcoin community isn't paying you to do bitcoin things | 06:55 |
cynsia | cool | 06:55 |
cynsia | I have a lot of ideas for that | 06:55 |
cynsia | if only I had the time... | 06:55 |
seanph | @cynsia ideas for what? getting thru the great firewall? | 06:56 |
cynsia | I feel like there should be a more asynchronous, at a lower level | 06:56 |
cynsia | especially for distributed mesh networks | 06:56 |
cynsia | we should have message queuing that is designed for that, from the bottom up | 06:56 |
cynsia | because the web is made with the assumption that there's an always on, fast connection | 06:56 |
cynsia | sadly | 06:56 |
ebowden_ | http://explosm.net/comics/3479/ | 06:59 |
seanph | @cynsia well, one of the goals of this project is to create a distributed content addressable storage | 06:59 |
seanph | @cynsia I think that covers a lot of the use cases for "slow data" | 07:00 |
kanzure | have you read the source code to tahoe-lafs and ipfs? | 07:00 |
seanph | @kanzure nope, I've not actually started on that part of the project yet. still getting the real time communications in place | 07:00 |
cynsia | ipfs is wonderful | 07:00 |
seanph | @kanzure will make notes of them tho | 07:00 |
cynsia | I just think about it a lot, because I'm often on a spotty connection | 07:01 |
seanph | @cynsia one of the things that this enables you to do is to use several connections at once, and several different routes | 07:01 |
seanph | @cynsia we have Linux and OSX applications that give you a tun interface that lets you tunnel traffic thru the mesh network, which may then take different routes etc | 07:03 |
cynsia | cool | 07:03 |
cynsia | I've been experimenting with my own socks server | 07:03 |
FourFire | kanzure, your project is a delivery method for gene mods? | 07:04 |
kanzure | https://github.com/darkk/redsocks | 07:04 |
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kanzure | FourFire: synthetic virus stuff? eventually an app store but for now it's not my project, it's someone else's. | 07:05 |
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FourFire | ok. | 07:07 |
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kanzure | part dog http://xkcd.com/1706/ | 07:12 |
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kanzure | .wik dennō coil | 07:18 |
yoleaux | "Dennō Coil (電脳コイル, Dennō Koiru?, lit. Electric Brain Coil or Computer Coil), Coil—A Circle of Children, is a Japanese science fiction anime television series depicting a near future where semi-immersive augmented reality (AR) technology has just begun to enter the mainstream." — https://en.wikipedia.org/wiki/Denn%C5%8D_Coil | 07:18 |
kanzure | from https://groups.google.com/forum/#!topic/diytranshumanist/UKKndKfJky8 (which is itself a strange email...) | 07:19 |
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fenn | denno coil is like SL1 | 08:34 |
fenn | the big reveal is that it started out as a medical device | 08:35 |
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strages | my favorite part about denno coil was the use of antiquated glyphs to manipulate the augmented reality layer due to an older version of the system still being partially in place | 08:49 |
maaku | hrm. i wonder if there are japanese subs for dennō | 08:50 |
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nmz787_i | TMA: nope no CT included | 09:42 |
nmz787_i | abetusk: nope, haven't had time since I last talked with you | 09:42 |
nmz787_i | either I am super duper lazy, or I just am super duper unimpressed with social interaction... because I barely feel like performing responsibilities that entail physically travelling and waiting for other humans while I am away. Even just calling dentists to make appointments is disinteresting... | 09:49 |
nmz787_i | unfortunately I can't read this here at work... oh where is our papercopier bot? http://onlinelibrary.wiley.com.sci-hub.cc/doi/10.1002/marc.200300191/abstract | 10:03 |
nmz787_i | “Synthesis and Thermal Properties of Epoxidized Vegetable Oil” | 10:03 |
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xentrac | nmz787_i: can you use Tor? | 10:05 |
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nmz787_i | eh, no idea... probably against policy and could get me fired | 10:08 |
xentrac | oh, really? | 10:10 |
xentrac | can you use a personal cellphone with a data connection? or are you working in a SCIF or something? | 10:10 |
nmz787_i | sure | 10:10 |
nmz787_i | I can just wait til later too | 10:10 |
nmz787_i | my job has nothing to do with veggie oil epoxification... so it isn't really work-related other than general science/engineering technique | 10:11 |
nmz787_i | so hard to justify going too far out of my way to get it | 10:11 |
nmz787_i | I could alternatively just disconnect from work's VPN and it would work | 10:12 |
nmz787_i | hmm, maybe there are some dentists on craigslist | 10:24 |
nmz787_i | backalley CT is what I need | 10:24 |
nmz787_i | TMA: just heard a tip about a local place offering CT for $300... but I still need a damn dentist/doctor referral | 10:25 |
nmz787_i | hmm, I wonder if that TOR marketplace site has Dr referrals for sale... | 10:26 |
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chris_99 | nmz787_i, got my 'optical table' just awaiting some rods to use for pegs | 11:19 |
xentrac | is it steel? | 11:22 |
chris_99 | alas not, just plastic, i figure i can always make something better later, this is just a test with the Pi camera, i'll upgrade to a line ccd if it works | 11:23 |
kanzure | god damn it fenn, spoiling it for everyone :P | 11:24 |
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nmz787_i | hmm, maybe Native American reservations have less-restricted doctors/dentists | 11:25 |
nmz787_i | hmm, native americans need me to be registered with the reservation | 11:28 |
nmz787_i | .title http://www.joms.org/article/S0278-2391(15)01418-4/abstract | 11:30 |
yoleaux | nmz787_i: Sorry, that doesn't appear to be an HTML page. | 11:30 |
xentrac | plastics have a TCE over an order of magnitude higher than that of steel, whose TCE is an order of magnitude higher than that of granite | 11:31 |
nmz787_i | just gotta setup a SAT solver to find the correct sequence of input I need to feed the doctor, to get a referral out the other end of this spec | 11:31 |
xentrac | similarly for plastics' compliance | 11:31 |
xentrac | also they're mostly hygroscopic | 11:31 |
chris_99 | xentrac, i'll just be impressive if i can even find a peak at 900nm tbh, so i'm not too worried about creep atm | 11:31 |
chris_99 | *impressed | 11:32 |
xentrac | but maybe you don't care | 11:32 |
xentrac | creep is a different thing | 11:32 |
chris_99 | wait, what's TCE stand for | 11:33 |
xentrac | thermal coefficient of expansion | 11:38 |
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c0rw1n | .title http://qz.com/722614/a-civil-servant-missing-most-of-his-brain-challenges-our-most-basic-theories-of-consciousness/ | 12:22 |
yoleaux | A civil servant missing most of his brain challenges our most basic theories of consciousness — Quartz | 12:22 |
c0rw1n | it's the second case i read about. does anyone have the link to the other one and how likely do you think it is that it's actually the same and the article got confused? | 12:23 |
kanzure | it's just squished up in his head | 12:29 |
c0rw1n | huh. so what's the limit of squishing? i mean, how many brains can actually fit in one head? | 12:31 |
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maaku | Define brain. | 12:45 |
maaku | Is it clear whether he has any deficiencies, even subtle ones? | 12:45 |
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nmz787_i | fenn: you may be interested in 'andronium' and/or 'superbook' | 13:19 |
nmz787_i | seems to be some sort of USB-based laptop screen+keyboard | 13:19 |
nmz787_i | but it seems to be missing a dock/holder for the phone, as far as I can tell | 13:20 |
chris_99 | know what res the screen is | 13:20 |
nmz787_i | says HD, probably fake-HD (1366x768) | 13:21 |
chris_99 | ah | 13:21 |
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kanzure | maaku: 75 iq | 13:29 |
kanzure | which is not bad | 13:29 |
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kanzure | "The departing directors are Meredith Hoban Dunn, Ian Goldberg, Julius Mittenzwei, Rabbi Rob Thomas, Wendy Seltzer and two of Tor’s co-founders, Roger Dingledine and Nick Mathewson. Mr. Dingledine and Mr. Mathewson will remain as leaders of Tor’s technical research and development." | 13:39 |
kanzure | why would mathewson "depart"? wat? | 13:39 |
kanzure | this is from http://www.nytimes.com/2016/07/14/technology/tor-project-a-digital-privacy-group-reboots-with-new-board.html | 13:39 |
kanzure | and wendy is from w3c i think. and coleman was at scaling bitcoin 1. | 13:39 |
kanzure | "Reversible cryo-arrest for imaging molecules in living cells at high spatial resolution" http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3921.html | 13:41 |
kanzure | above is "time dilation microscopy" | 13:41 |
kanzure | "The dynamics of molecules in living cells hampers precise imaging of molecular patterns by functional and super-resolution microscopy. We developed a method that circumvents lethal chemical fixation and allows on-stage cryo-arrest for consecutive imaging of molecular patterns within the same living, but arrested, cells. The reversibility of consecutive cryo-arrests was demonstrated by the high survival rate of different cell lines and ... | 13:42 |
kanzure | ... by intact growth factor signaling that was not perturbed by stress response. Reversible cryo-arrest was applied to study the evolution of ligand-induced receptor tyrosine kinase activation at different scales. The nanoscale clustering of epidermal growth factor receptor (EGFR) in the plasma membrane was assessed by single-molecule localization microscopy, and endosomal microscale activity patterns of ephrin receptor A2 (EphA2) were ... | 13:42 |
kanzure | ... assessed by fluorescence lifetime imaging microscopy. Reversible cryo-arrest allows the precise determination of molecular patterns while conserving the dynamic capabilities of living cells." | 13:42 |
kanzure | "Janelia/MIT Expansion Microscopy workshop, Aug 14-19, 2016" https://www.janelia.org/you-janelia/conferences/expansion-microscopy-workshop | 13:43 |
kanzure | NIH brain initiative needs a director https://jobs.nih.gov/vacancies/executive/brain.htm | 13:45 |
kanzure | .tw https://twitter.com/LauraBeen1/status/740988104224460800 | 13:45 |
yoleaux | The Been Lab just expanded our 1st brain! Thanks @eboyden3 for hooking us up with ExTBio. Students & I are excited! https://t.co/HFEDY0YPi9 (@LauraBeen1) | 13:45 |
kanzure | ginkgo bioworks has recently raised $100M | 13:46 |
kanzure | .tw https://twitter.com/eboyden3/status/740230401399001088 | 13:46 |
yoleaux | MIT Media Lab professor Kevin Esvelt featured in NYTimes for his proposal to engineer mice to halt Lyme disease. http://www.nytimes.com/2016/06/08/science/ticks-lyme-disease-mice-nantucket.html (@eboyden3) | 13:46 |
kanzure | "Multiplexed neural recording along a single optical fiber via optical reflectometry" http://caps.luminad.com:8080/stockage/stock/LDL-SPIE-JBO-160052R/JBO-160052R_online.pdf | 13:47 |
kanzure | "We introduce the design and theoretical analysis of a fiber-optic architecture for neural recording without contrast agents, which transduces neural electrical signals into a multiplexed optical readout. Our sensor design is inspired by electro-optic modulators, which modulate the refractive index of a waveguide by applying a voltage across an electro-optic core material. We estimate that this design would allow recording of the ... | 13:49 |
kanzure | ... activities of individual neurons located at points along a 10-cm length of optical fiber with 40-μm axial resolution and sensitivity down to 100 μV using commercially available optical reflectometers as readout devices." | 13:49 |
kanzure | "Neural recording sites detect a potential difference against a reference and apply this potential to a capacitor. The wave-guide serves as one of the plates of the capacitor, so charge accumulation across the capacitor results in an optical effect. A key concept of the design is that the sensitivity can be improved by increasing the capacitance. To maximize the capacitance, we utilize a microscopic layer of material with high relative ... | 13:49 |
kanzure | ... permittivity. If suitable materials can be found—possessing high capacitance per unit area as well as favorable properties with respect to toxicity, optical attenuation, ohmic junctions, and surface capacitance—then such sensing fibers could, in principle, be scaled down to few-micron cross-sections for minimally invasive neural interfacing." | 13:50 |
kanzure | "Could a neuroscientist understand a microprocessor?" http://biorxiv.org/content/early/2016/05/26/055624 | 13:50 |
kanzure | ( http://biorxiv.org/content/early/2016/05/26/055624.full.pdf ) | 13:51 |
kanzure | "There is a popular belief in neuroscience that we are primarily data limited, that producing large, multimodal, and complex datasets will, enabled by data analysis algorithms, lead to fundamental insights into the way the brain processes information. Microprocessors are among those artificial information processing systems that are both complex and that we understand at all levels, from the overall logical flow, via logical gates, to ... | 13:51 |
kanzure | ... the dynamics of transistors. Here we take a simulated classical microprocessor as a model organism, and use our ability to perform arbitrary experiments on it to see if popular data analysis methods from neuroscience can elucidate the way it processes information. We show that the approaches reveal interesting structure in the data but do not meaningfully describe the hierarchy of information processing in the processor. This ... | 13:51 |
kanzure | ... suggests that current approaches in neuroscience may fall short of producing meaningful models of the brain." | 13:51 |
kanzure | ( https://twitter.com/stochastician/status/735911424879300608 ) | 13:51 |
kanzure | on the 15th at stanford there will be a discussion about the above paper, https://neuroscience.stanford.edu/events/could-neuroscientist-understand-microprocessor-eric-jonas | 13:52 |
kanzure | overview of instruments on the juno spacecraft http://spaceflight101.com/juno/instrument-overview/ | 13:53 |
kanzure | (human motion) "Chunking as the result of an efficiency computation trade-off" http://www.nature.com/ncomms/2016/160711/ncomms12176/full/ncomms12176.html | 13:53 |
kanzure | "pip install crnsget" for computational neuroscience data fetching tool http://crcns.org/ | 13:54 |
kanzure | science search engine thingy http://pubmed.scholarfy.net/ | 13:54 |
kanzure | "Kilosort: realtime spike-sorting for extracellular electrophysiology with hundreds of channels" http://biorxiv.org/content/early/2016/06/30/061481 | 13:55 |
kanzure | https://github.com/cortex-lab/Kilosort | 13:55 |
kanzure | "A simple proposal for the publication of journal citation distributions" http://biorxiv.org/content/early/2016/07/05/062109 | 13:56 |
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kanzure | https://forbetterscience.wordpress.com/2016/04/07/voinnet-aftermath-ethical-bankruptcy-of-academic-elites/ | 13:59 |
kanzure | spike analysis and visualization stuff https://github.com/KordingLab/spykes | 13:59 |
kanzure | china research investment trends http://www.nature.com/news/china-by-the-numbers-1.20122 | 13:59 |
kanzure | "Voodoo Machine Learning for Clinical Predictions" http://biorxiv.org/content/early/2016/06/19/059774 | 14:00 |
kanzure | "Optimal real-time landing using deep networks" https://indico.esa.int/indico/event/111/session/28/contribution/147/material/paper/0.pdf (for spacecraft landings) | 14:00 |
kanzure | "Leaping eels electrify threats, supporting Humboldt’s account of a battle with horses" http://www.pnas.org/content/113/25/6979.full | 14:01 |
kanzure | "Electric eels are shown to leap from the water to directly electrify threats. This shocking behavior likely allows electric eels to defend themselves during the Amazonian dry season, when they may be found in small pools and in danger of predation. The results support Alexander von Humboldt’s story of electric eels attacking horses that had been herded into a muddy pool during the dry season in 1800. The finding highlights ... | 14:01 |
kanzure | ... sophisticated behaviors that have evolved in concert with the eel’s powerful electrical organs." | 14:01 |
kanzure | https://github.com/Enegnei/JacobAppelbaumLeavesTor/blob/master/JacobAppelbaumLeavesTor.md | 14:04 |
kanzure | guidance source code for apollo 11 https://github.com/chrislgarry/Apollo-11 | 14:04 |
kanzure | .tw https://twitter.com/tsai_ming_lu/status/747631276962816000 | 14:04 |
yoleaux | DAPI & Phalloidine staining on Tardegrade @MBLScience #embryo2016 https://t.co/YS4Cjou53l (@tsai_ming_lu) | 14:04 |
kanzure | .tw https://twitter.com/IchaJaroslav/status/744885322769698816 | 14:05 |
yoleaux | Two days old zebrafish embryo stained for acetylated tubulin and DNA https://t.co/ysOEFXw7b8 (@IchaJaroslav) | 14:05 |
kanzure | mitochondrial replacement therapy proposals http://www.ipscell.com/2016/06/mitochondrial-replacement-hype-goes-nuclear-including-by-wellcome-trust/ | 14:06 |
kanzure | "Virtual finger boosts three-dimensional imaging and microsurgery as well as terabyte volume image visualization and analysis" http://www.nature.com/ncomms/2014/140711/ncomms5342/full/ncomms5342.html | 14:08 |
kanzure | "Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for ... | 14:08 |
kanzure | ... acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. ... | 14:09 |
kanzure | ... We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain." | 14:09 |
kanzure | looks like they are computing different projection curves and then snapping hand motions and other gestures to those precomputed trajectories | 14:09 |
kanzure | "Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos" http://biorxiv.org/content/early/2016/07/05/062182 | 14:10 |
kanzure | "Nanoscale imaging of RNA with expansion microscopy" http://syntheticneurobiology.org/PDFs/16.07.chen.FULL.pdf | 14:10 |
kanzure | "The ability to image RNA identity and location with nanoscale precision in intact tissues is of great interest for defining cell types and states in normal and pathological biological settings. Here, we present a strategy for expansion microscopy of RNA. We developed a small-molecule linker that enables RNA to be covalently attached to a swellable polyelectrolyte gel synthesized throughout a biological specimen. Then, postexpansion, ... | 14:11 |
kanzure | ... fluorescent in situ hybridization (FISH) imaging of RNA can be performed with high yield and specificity as well as single-molecule precision in both cultured cells and intact brain tissue. Expansion FISH (ExFISH) separates RNAs and supports amplification of single-molecule signals (i.e., via hybridization chain reaction) as well as multiplexed RNA FISH readout. ExFISH thus enables super-resolution imaging of RNA structure and ... | 14:11 |
kanzure | ... location with diffraction-limited microscopes in thick specimens, such as intact brain tissue and other tissues of importance to biology and medicine." | 14:11 |
kanzure | "Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies" http://syntheticneurobiology.org/PDFs/16.07.tillberg.FULL.pdf | 14:12 |
kanzure | "ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and ... | 14:13 |
kanzure | ... streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (~70 nm) imaging of cells and mammalian tissues on conventional microscopes." | 14:13 |
kanzure | figure 3f is particularly crazy | 14:13 |
kanzure | .title https://www.youtube.com/watch?v=2A-4OtRaSpM | 14:14 |
yoleaux | Expansion Microscopy of Brainbow Hippocampus | 14:14 |
kanzure | .tw https://twitter.com/AndrewHires/status/737318370966568960 | 14:20 |
yoleaux | That's nearly an entire hemisphere of mouse cortex in panel A, with random access scanning everywhere across it. https://t.co/DyooAUDw2V (@AndrewHires, in reply to tw:737312723801767936) | 14:20 |
kanzure | "Fighting autoimmunity with immune cells" http://science.sciencemag.org/content/353/6294/14 | 14:23 |
kanzure | "The approach relies on chimeric antigen receptor T cells, or CAR T cells: immune cells genetically modified to home in on a desired target on cancer cells or—in this case—on rogue B cells, another immune cell type. The new study only gauged the CAR T cells' capabilities in the lab dish and in mouse models of pemphigus vulgaris, an autoimmune condition in which B cells secrete antibodies that attack a protein in skin and mucous ... | 14:23 |
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kanzure | ... membrane." | 14:23 |
kanzure | some sort of startup doing synthetic leather production? http://modernmeadow.com/ | 14:23 |
kanzure | "Precise Cas9 targeting enables genomic mutation prevention" http://biorxiv.org/content/early/2016/06/14/058974 | 14:24 |
kanzure | "Here we present a generalized method of guide RNA tuning that enables Cas9 to discriminate between two target sites that differ by a single nucleotide polymorphism. We employ our methodology to generate a novel in vivo mutation prevention system in which Cas9 actively restricts the occurrence of undesired gain-of-function mutations within a population of engineered organisms. We further demonstrate that the system is scalable to a ... | 14:24 |
kanzure | ... multitude of targets and that the general tuning and prevention concepts are portable across engineered Cas9 variants and Cas9 orthologs. Finally, we show that the designed mutation prevention system maintains robust activity even when placed within the complex environment of the mouse gastrointestinal tract." | 14:24 |
kanzure | "Daisy-chain gene drives for the alteration of local populations" http://www.responsivescience.org/pub/daisydrives | 14:24 |
kanzure | "First CRISPR clinical trial gets green light from US panel" http://www.nature.com/news/first-crispr-clinical-trial-gets-green-light-from-us-panel-1.20137 | 14:25 |
kanzure | "Mirror-enhanced super-resolution microscopy" http://www.nature.com/lsa/journal/v5/n6/full/lsa2016134a.html | 14:26 |
kanzure | aka "put a mirror on your coverslip and gain 4X axial resolution in confocal and STED microscopy" | 14:26 |
kanzure | "Birds have primate-like numbers of neurons in the forebrain" http://www.pnas.org/content/113/26/7255.abstract | 14:28 |
kanzure | "Corvids and some parrots are capable of cognitive feats comparable to those of great apes. How do birds achieve impressive cognitive prowess with walnut-sized brains? We investigated the cellular composition of the brains of 28 avian species, uncovering a straightforward solution to the puzzle: brains of songbirds and parrots contain very large numbers of neurons, at neuronal densities considerably exceeding those found in mammals. ... | 14:29 |
kanzure | ... Because these “extra” neurons are predominantly located in the forebrain, large parrots and corvids have the same or greater forebrain neuron counts as monkeys with much larger brains. Avian brains thus have the potential to provide much higher “cognitive power” per unit mass than do mammalian brains." | 14:29 |
kanzure | this seems like something we should have known about earlier | 14:29 |
kanzure | i suppose we can chalk that up to the traditional "of course humans are better" attitudes that prevented anyone from investigating animal neuroscience earlier in our history | 14:30 |
kanzure | s/better/more sophisticated | 14:30 |
kanzure | s/more sophisticated/more interesting | 14:30 |
kanzure | "Evolution of resistance against CRISPR/Cas9 gene drive" http://biorxiv.org/content/early/2016/06/11/058438 | 14:31 |
kanzure | http://www.nature.com/news/gene-editing-can-drive-science-to-openness-1.20043 | 14:31 |
kanzure | "Cell lineage tracing using nuclease barcoding" http://arxiv.org/pdf/1606.00786v1 | 14:32 |
kanzure | "The strategy we use creates lineage trees based upon the introduction of specific mutations into cells and the propagation of these mutations to daughter cells at each cell division. We present an experimental proof of concept along with a corresponding simulation and analytical model for deeper understanding of the coding capacity of the system. By introducing mutations in a predictable manner using CRISPR/Cas9, our technology will ... | 14:32 |
kanzure | ... enable more complete investigations of cellular processes." | 14:32 |
kanzure | strange that this seems to be from the quake lab (microfluidics stuff) | 14:33 |
kanzure | "Brain heating induced by near infrared lasers during multi-photon microscopy" http://biorxiv.org/content/early/2016/06/06/057364 | 14:34 |
kanzure | "Here we used thermocouple probes and quantum dot nanothermometers to measure temperature changes induced by two-photon microscopy in the neocortex of awake and anaesthetized mice. We characterized heating as a function of wavelength, exposure time, and distance from the center of illumination. Although total power is highest near the surface of the brain, heating was most severe hundreds of microns below the focal plane, due to heat ... | 14:34 |
kanzure | ... dissipation through the cranial window." | 14:34 |
kanzure | "Continuous illumination of a 1mm2 area produced a peak temperature increase of approximately 1.8°C/100mW. Continuous illumination with powers above 250 mW induced lasting damage, detected with immunohistochemistry against Iba1, GFAP, heat shock proteins, and activated Caspase-3. Higher powers were usable in experiments with limited duty ratios, suggesting an approach to mitigate damage in high-power microscopy experiments." | 14:34 |
kanzure | "High-throughput mapping of single neuron projections by sequencing of barcoded RNA" http://biorxiv.org/content/early/2016/05/20/054312 | 14:35 |
kanzure | "Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences ("barcodes"). Axons are filled with barcode mRNA, each putative projection area is dissected, and the barcode mRNA is extracted and sequenced. Applying MAPseq to the locus coeruleus (LC), we find that individual LC ... | 14:35 |
kanzure | ... neurons have preferred cortical targets. By recasting neuroanatomy, which is traditionally viewed as a problem of microscopy, as a problem of sequencing, MAPseq harnesses advances in sequencing technology to permit high-throughput interrogation of brain circuits." | 14:36 |
kanzure | ("neural barcodes" i guess) | 14:36 |
kanzure | there was a similar proposal from a while back... where did that one go? | 14:36 |
kanzure | oh i think the previous proposals were about multiplexed fluorescent markers | 14:36 |
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kanzure | i am thinking of the "rosetta brains" paper which used "Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC)" https://arxiv.org/abs/1404.5103 | 14:39 |
kanzure | and not the brainbow fluorophore tagging stuff http://diyhpl.us/~bryan/papers2/neuro/On%20optical%20detection%20of%20densely%20labeled%20synapses%20in%20neuropil%20and%20mapping%20connectivity%20with%20combinatorially%20multiplexed%20fluorescent%20synaptic%20markers%20-%20Mishchenko%20-%202010.pdf | 14:40 |
kanzure | oh okay it is cited by the RNA barcoding paper ("multiplexed analysis of projections by sequencing") | 14:41 |
kanzure | yeah that is probably the coolest thing ever | 14:44 |
kanzure | reducing this to a sequencing problem is an excellent idea because we have become very good at dna sequencing | 14:47 |
kanzure | anatomically i doubt any of the physical structures are going to surprise us, it's only the connection data that we seem to be looking for at this point | 14:47 |
kanzure | local concentration of proteins and receptors could be physically recorded through other means (such as by threshold counting of mRNA transcripts floating around in each neuron by some enzyme or regulatory network), and then perhaps attached to each of the barcodes (although this is a long-term project) | 14:48 |
kanzure | which would record important synaptic weighting information on multiple dimensions | 14:49 |
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kanzure | i bet you could use viruses for barcoding | 14:58 |
kanzure | yup "viral genetic barcoding" seems to be a thing | 14:58 |
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kanzure | "30 percent of all protein adaptations since humans' divergence with chimpanzees have been driven by viruses" | 16:11 |
kanzure | "Viruses are a dominant driver of protein adaptation in mammals" https://elifesciences.org/content/5/e12469 | 16:11 |
kanzure | "In a 1964 paper Exit icon, John Platt codified an alternative approach to the standard conception of the scientific method, which he named strong inference. In strong inference, scientists always produce multiple hypotheses that will explain their data and then design experiments that will distinguish among these alternative hypotheses. The advantage, at least in principle, is that it forces us to consider different explanations for our ... | 16:14 |
kanzure | ... results at every stage, minimizing confirmation bias and tunnel vision." | 16:14 |
kanzure | from https://loop.nigms.nih.gov/2014/03/hypothesis-overdrive/ | 16:14 |
kanzure | "strong inference" paper is http://science.sciencemag.org/content/146/3642/347.extract | 16:14 |
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* kanzure makes a note to name his method "hyperstrong inference" | 16:15 | |
kanzure | "Reentrancy woes in smart contracts" http://hackingdistributed.com/2016/07/13/reentrancy-woes/ | 16:15 |
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c0rw1n | hah, subtle. <3 | 16:18 |
kanzure | "Design of a hyperstable 60-subunit protein icosahedron" http://www.nature.com/nature/journal/v535/n7610/full/nature18010.html | 16:21 |
kanzure | "Here we describe the computational design of a 25-nanometre icosahedral nanocage that self-assembles from trimeric protein building blocks. The designed protein was produced in Escherichia coli, and found by electron microscopy to assemble into a homogenous population of icosahedral particles nearly identical to the design model. The particles are stable in 6.7 molar guanidine hydrochloride at up to 80 degrees Celsius, and undergo ... | 16:21 |
kanzure | ... extremely abrupt, but reversible, disassembly between 2 molar and 2.25 molar guanidinium thiocyanate. The icosahedron is robust to genetic fusions: one or two copies of green fluorescent protein (GFP) can be fused to each of the 60 subunits to create highly fluorescent ‘standard candles’ for use in light microscopy, and a designed protein pentamer can be placed in the centre of each of the 20 pentameric faces to modulate the size ... | 16:21 |
kanzure | ... of the entrance/exit channels of the cage. Such robust and customizable nanocages should have considerable utility in targeted drug delivery6, vaccine design7 and synthetic biology8." | 16:21 |
kanzure | "Whole organism lineage tracing by combinatorial and cumulative genome editing" http://biorxiv.org/content/early/2016/05/11/052712 | 16:34 |
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kanzure | "Here we use genome editing to progressively introduce and accumulate diverse mutations in a DNA barcode over multiple rounds of cell division. The barcode, an array of CRISPR/Cas9 target sites, records lineage relationships in the patterns of mutations shared between cells. In cell culture and zebrafish, we show that rates and patterns of editing are tunable, and that thousands of lineage-informative barcode alleles can be generated. By ... | 16:39 |
kanzure | ... sampling hundreds of thousands of cells from individual zebrafish, we find that most cells in adult zebrafish organs derive from relatively few embryonic progenitors. Genome editing of synthetic target arrays for lineage tracing (GESTALT) will help generate large-scale maps of cell lineage in multicellular systems." | 16:39 |
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kanzure | "Detection of human adaptation during the past 2,000 years" http://biorxiv.org/content/early/2016/05/07/052084 | 16:46 |
kanzure | "Three decades of nanopore sequencing" http://www.nature.com/nbt/journal/v34/n5/abs/nbt.3423.html | 16:47 |
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kanzure | "Detecting DNA Methylation using the Oxford Nanopore Technologies MinION sequencer" http://biorxiv.org/content/early/2016/04/04/047142 | 16:48 |
kanzure | .title https://www.src.org/calendar/e006043/ | 16:49 |
yoleaux | IARPA/SRC Workshop on DNA-based Massive Information Storage (invitation only) (Event E006043) - SRC | 16:49 |
kanzure | http://www.prepubmed.org/ "PrePubMed indexes preprints from arXiv q-bio, PeerJ Preprints, Figshare, bioRxiv, F1000Research, and The Winnower. Articles are not stored on PrePubMed, but you will be linked to the article at the respective site." | 16:49 |
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kanzure | .tw https://twitter.com/jlee8usa/status/720724842744573953 | 16:51 |
yoleaux | In situ RNA seq (FISSEQ). Crazy nuclear blebbing seen in some neural cells. What is going on? (Thx Debarati) https://t.co/X3xKKx269F (@jlee8usa) | 16:51 |
kanzure | "Prevalence, phenotype and architecture of developmental disorders caused by de novo mutation" http://biorxiv.org/content/early/2016/04/20/049056 | 16:54 |
kanzure | "Deep Sequencing of 10,000 Human Genomes" http://biorxiv.org/content/early/2016/07/01/061663 | 16:56 |
kanzure | "We report on the sequencing of 10,545 human genomes at 30-40x coverage with an emphasis on quality metrics and novel variant and sequence discovery. Each newly sequenced genome contributes an average of 8,579 novel variants. In addition, each genome carries in average 0.7 Mb of sequence that is not found in the main build of the hg38 reference genome. The density of this catalog of variation allowed us to construct high resolution ... | 16:56 |
kanzure | ... profiles that define genomic sites that are highly intolerant of genetic variation." | 16:56 |
kanzure | https://www.edge.org/conversation/george_church-the-augmented-human-being | 16:58 |
kanzure | ah that was from march. we already saw that. | 16:58 |
kanzure | http://www.cureffi.org/2016/06/28/introductory-reading-list/ | 16:58 |
kanzure | .title http://www.cureffi.org/2016/06/28/introductory-reading-list/ | 16:58 |
yoleaux | Introductory prion reading list | 16:58 |
kanzure | "The bacteriophage ϕ29 tail possesses a pore-forming loop for cell membrane penetration" http://www.nature.com/nature/journal/vaop/ncurrent/full/nature18017.html | 16:59 |
kanzure | "The mechanisms involved in the penetration of the inner host cell membrane by bacteriophage tails are not well understood. Here we describe structural and functional studies of the bacteriophage ϕ29 tail knob protein gene product 9 (gp9). The 2.0 Å crystal structure of gp9 shows that six gp9 molecules form a hexameric tube structure with six flexible hydrophobic loops blocking one end of the tube before DNA ejection. Sequence and ... | 17:00 |
kanzure | ... structural analyses suggest that the loops in the tube could be membrane active. Further biochemical assays and electron microscopy structural analyses show that the six hydrophobic loops in the tube exit upon DNA ejection and form a channel that spans the lipid bilayer of the membrane and allows the release of the bacteriophage genomic DNA, suggesting that cell membrane penetration involves a pore-forming mechanism similar to that ... | 17:00 |
kanzure | ... of certain non-enveloped eukaryotic viruses2, 3, 4." | 17:00 |
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nmz787_i | I wonder when someone will come out will some kind of mouth-guard thing with UV lights/LEDs/lasers pointing at the teeth to kill bacteria | 17:13 |
kanzure | bacteria in the mouth are fine as long as you can pick and choose which of them you keep | 17:14 |
kanzure | personalized mouth bacteria (mouthomes?) could conceivably keep out the nasty | 17:15 |
nmz787_i | this is pretty neat, bacterial fluorescense http://www.dentistrytoday.com/diagnosis/4232-caries-visualization-with-fluorescent-technology | 17:15 |
kanzure | i think there has been at least one igem-related dentistry project | 17:15 |
nmz787_i | kanzure: hmm, mouth bacteria sure, but teeth bacteria? | 17:15 |
kanzure | "ecoli to fight tooth decay by killing Streptococcus mutans" http://2013.igem.org/Team:UT_Dallas | 17:16 |
nmz787_i | /me has a fiber-coupled NIR laser | 17:16 |
nmz787_i | /me considering jamming the end of the fiber into my chipped tooth and turning it on | 17:17 |
nmz787_i | /me doesn't want to burn mouth/gums though | 17:17 |
nmz787_i | (or burning the tooth nerve I guess could be possible) | 17:17 |
kanzure | nah that's just a new form of cauterization | 17:17 |
nmz787_i | hrmm | 17:17 |
nmz787_i | seems like I got a different dentist to take my money for a CT scan | 17:18 |
kanzure | you'll be fine, and if you do something wrong you could always chip another tooth | 17:18 |
kanzure | you have an entire set of teeth to work with (presumably) | 17:18 |
nmz787_i | reverse-engineering the file format may be needed, we shall see | 17:18 |
kanzure | yep this is doable | 17:19 |
kanzure | sometimes those programs are so evil that they even include viewer software on the CDs that they burn | 17:19 |
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nmz787_i | that would be pretty sweet if so | 17:21 |
kanzure | i'm pretty sure at least some of the medical equipment that does that is also installing malware | 17:21 |
nmz787_i | sweet in a terrible way, like how sugar feeds teeth bacteria | 17:21 |
kanzure | it's a good distribution vector | 17:21 |
kanzure | i wonder if wearing a retainer while drinking sugary drinks would be enough to prevent most tooth decay | 17:22 |
nmz787_i | man, think of all the nerds drinking SURGE that you could sell that too, the guys who already have pocket protectors most likely | 17:24 |
nmz787_i | blast this tooth with a laser for a few days... then pack some vacuum-seal compatible epoxy in there... should be fine, right? | 17:24 |
nmz787_i | (torr-seal because, you don't want the tooth bacteria to get oxygen that could permeate with other cementacious compounds) | 17:25 |
nmz787_i | "This result make us to use Diode-Pumped Solid State Laser (DPSSL) with a wavelength of (405nm), power of its laser was 20 mw. The result showed that the time required to kill the bacteria was about (22min)." | 17:26 |
nmz787_i | "Effect of 405 nm Laser Light on the Cariogenic Bacteria Streptococcus Mutans" | 17:26 |
c0rw1n | so in the glorious transhuman future we brush our teeth with light. noted :D | 17:27 |
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kanzure | probably you wouldn't need to brush teeth because either regrowing sets of teeth or mouth bacteria ecosystem would fight off infections | 17:31 |
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kanzure | sheena says mouse care is $1/mouse/day at low scale.... so a million mice would be $1M/day (probably less because of economies of scale, but who knows). | 17:46 |
streety | a quick search suggests a roughly similar cost from a few different universities. It might be useful to find out what the main drivers of that cost are. | 18:02 |
streety | I suspect 50%+ is due to staffing | 18:02 |
kanzure | robots everywhere. got it. | 18:06 |
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maaku | kanzure: i assume automation more than economies of scale will bring that price down | 19:05 |
maaku | mouse care is a manual process these days, no? | 19:05 |
maaku | but i don't have any data to guess what the material cost is to maintain a clean living environment for a mouse, even with perfect automation | 19:06 |
maaku | electricity for lighting & mechanical contraptions, air conditioning, food and water, automation maintenance, etc.. | 19:07 |
kanzure | they are literally plastic bins sometimes with chicken wire floors to let the poop fall through | 19:07 |
kanzure | then you replace the mulch every day or every week | 19:07 |
kanzure | for the purpose of automation a different environment would probably be necessayr, or at least one where you can reasonably handle young pups to get them off of mulch while you replace | 19:08 |
kanzure | there have been some complaints about not enough space allocated per mouse in various animal testing facilities, which might impact ideas like using pvc pipes for housing millions of mice in an automated environment. perhaps some other automation will be required instead. :( | 19:09 |
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kanzure | .wik liu cixin | 19:17 |
yoleaux | "Liu Cixin (simplified Chinese: 刘慈欣; traditional Chinese: 劉慈欣, IPA: [li̯ǒu̯ tsʰɨ̌ɕín]; born 1963) is a Chinese science fiction writer. He is a nine-time winner of the Galaxy Award (China's most prestigious literary science fiction award) and winner of the Hugo Award. Liu's work is considered hard science fiction." — https://en.wikipedia.org/wiki/Liu_Cixin | 19:17 |
streety | I've never seen mouse or rat cages with wire floors, even in scenarios where you want to collect the feces and urine there seems to be a push away from wire floors. Always been solid plastic tanks with water and food suspended above | 19:18 |
kanzure | http://www.bfslattery.com/fiction.html | 19:21 |
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kanzure | .wik ship breaker | 19:23 |
yoleaux | "Ship breaking or ship demolition is a type of ship disposal involving the breaking up of ships for either a source of parts, which can be sold for re-use, or for the extraction of raw materials, chiefly scrap. It may also be known as ship dismantling, ship cracking, ship recycling, or ship disposal." — https://en.wikipedia.org/wiki/Ship_breaker | 19:23 |
kanzure | grr | 19:23 |
kanzure | https://en.wikipedia.org/wiki/Ship_Breaker | 19:23 |
kanzure | https://en.wikipedia.org/wiki/Yellow_Card_Man | 19:23 |
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kanzure | https://en.wikipedia.org/wiki/Spin_(novel) | 19:25 |
kanzure | https://www.amazon.com/Radiance-Novel-Catherynne-M-Valente/dp/0765335298 | 19:26 |
kanzure | .wik Ancillary Justice | 19:27 |
yoleaux | "Ancillary Justice is a science fiction novel by the American writer Ann Leckie, published in 2013. It is Leckie's debut novel and the first in her "Imperial Radch" space opera trilogy, followed by Ancillary Sword (2014) and Ancillary Mercy (2015)." — https://en.wikipedia.org/wiki/Ancillary_Justice | 19:27 |
kanzure | "The novel follows Breq, the sole survivor of a starship destroyed by treachery and the vessel of that ship's artificial consciousness, as she seeks revenge against the ruler of her civilization." | 19:27 |
kanzure | .wik The Quantum Thief | 19:28 |
yoleaux | "The Quantum Thief is the debut science fiction novel by Hannu Rajaniemi and the first novel in a trilogy featuring Jean le Flambeur." — https://en.wikipedia.org/wiki/The_Quantum_Thief | 19:28 |
kanzure | https://en.wikipedia.org/wiki/Engineering_Infinity | 19:29 |
Aurelius_Home | great book | 19:29 |
kanzure | you read it? | 19:29 |
Aurelius_Home | read the whole trilogy | 19:29 |
kanzure | https://www.amazon.com/Planesrunner-Everness-Book-One-McDonald/dp/1616145412 | 19:31 |
kanzure | https://www.ece.gatech.edu/faculty-staff-directory/hua-wang | 19:42 |
kanzure | http://pencerw.com/ | 19:43 |
kanzure | http://pencerw.com/feed/2016/6/13/an-industrial-map "On a lark a week or two ago, I started putting locations of industrial stuff (factories, research centers, corporate headquarters, etc) on a custom Google Map. Many of these I've been to, on either public or private tours; others I hope to visit in the future." | 19:44 |
kanzure | "Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice." http://www.ncbi.nlm.nih.gov/pubmed/24700859 (2014) | 19:48 |
kanzure | "Here, we describe an approach that circumvents central motor circuit pathology to restore specific skeletal muscle function. We generated murine embryonic stem cell-derived motor neurons that express the light-sensitive ion channel channelrhodopsin-2, which we then engrafted into partially denervated branches of the sciatic nerve of adult mice. These engrafted motor neurons not only reinnervated lower hind-limb muscles but also enabled ... | 19:48 |
kanzure | ... their function to be restored in a controllable manner using optogenetic stimulation." | 19:48 |
kanzure | "Beyond the brain: Optogenetic control in the spinal cord and peripheral nervous system." http://www.ncbi.nlm.nih.gov/pubmed/27147590 | 19:48 |
kanzure | "In this Review, we discuss challenges in using optogenetics to study the mammalian spinal cord and peripheral nervous system, synthesize common features that unite the work done thus far, and describe a route forward for the successful application of optogenetics to translational research beyond the brain." | 19:49 |
kanzure | both from https://news.ycombinator.com/user?id=siyer https://news.ycombinator.com/item?id=12078650 | 19:49 |
kanzure | other related things from same person http://stanford.edu/~smiyer/publications/ | 19:49 |
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kanzure | "Preprints for the life sciences" http://science.sciencemag.org/content/352/6288/899.full | 20:08 |
kanzure | "Synthesizing the preferred inputs for neurons in neural networks via deep generator networks" http://arxiv.org/pdf/1605.09304v1.pdf | 20:17 |
kanzure | cc maaku (particularly page 2 figure 1) | 20:17 |
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kanzure | seanph: sup? | 21:31 |
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nmz787 | streety: I think wire cages might be stressful? | 21:38 |
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nmz787 | kanzure: PVC pipes is an interesting idea... you might be able to have a ball/roller driven robot that can traverse between the diameter of the pipe, picking up pups as it goes along... then you turn on a shop-vac and flush some cleaning solutions + air-dry | 21:41 |
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kanzure | it was fenn's idea, fwiw | 21:47 |
kanzure | oh you are proposing a robot for pup pick-n-place | 21:48 |
kanzure | i hadn't considered that. i was figuring pups would just have to be flushed. but your way is better. | 21:48 |
kanzure | andrew wants to try a controlled polymerase in solution, free floating :| | 21:54 |
* kanzure sleeps | 21:54 | |
fenn | andromium http://static1.squarespace.com/static/57132d6e4d088e1808016fb5/t/574e14787c65e430dff5d393/1464734848285/Andromium_MarketingProposes2.png | 22:08 |
fenn | i'm surprised "superbook" isn't trademarked to death already | 22:08 |
fenn | also what happened to chromeOS? and why can't you run it on a phone? | 22:09 |
nmz787 | I think chromeOS is what the chromebooks use | 22:15 |
nmz787 | I don't know any details other than it is some sort of linux based thing | 22:15 |
nmz787 | not sure if it is built on some common ground as android | 22:16 |
nmz787 | and as far as I could tell from the links I sent, andronium is an app? | 22:16 |
fenn | i expect andromium will have similar problems as RemixOS http://arstechnica.com/gadgets/2015/12/android-on-the-desktop-not-really-good-but-better-than-youd-think/ | 22:30 |
fenn | i guess you could run debian-arm | 22:30 |
fenn | i don't really understand how the usb display works | 22:31 |
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nmz787 | http://www.eetimes.com/document.asp?doc_id=1330086&page_number=1 | 22:49 |
nmz787 | .title | 22:49 |
yoleaux | Startup Debuts Open Source SoCs | EE Times | 22:49 |
nmz787 | fenn: I didn't look into it, I just assumed it is some app that uses their custom hardware to act as a second USB screen+kb+mouse | 22:50 |
fenn | "Could a neuroscientist understand a microprocessor?" sounds exactly like "Can a biologist fix a radio?" | 22:54 |
nmz787 | does it say that in that article? | 23:00 |
nmz787 | I guess CS and instruction sets are quite different in details and minutiae | 23:01 |
nmz787 | (than neuroscience) | 23:01 |
nmz787 | (i.e. biologists poking worms and collecting data, thinking of fluorophores and blah blah is the water pure | 23:02 |
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FourFire | Has Calico done anything useful yet? | 23:34 |
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