| --- Log opened Mon Jul 18 00:00:00 2016 | ||
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:1cf5:3e62:b786:253e] has joined ##hplusroadmap | 00:05 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has quit [Ping timeout: 252 seconds] | 01:19 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:1cf5:3e62:b786:253e] has quit [Remote host closed the connection] | 01:26 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 01:35 | |
| -!- augur [~augur@2602:304:cdac:e260:44f6:295a:746e:a7c8] has quit [Remote host closed the connection] | 02:05 | |
| chris_99 | heh nmz787, it looks good. Does anyone recall some kind of reverse engineering game | 02:07 |
|---|---|---|
| chris_99 | involving electronics | 02:07 |
| chris_99 | i can't seem to recall the name | 02:07 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-nbegqbhixxmpqnhr] has joined ##hplusroadmap | 02:17 | |
| -!- Aurelius_Home [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has quit [Read error: Connection reset by peer] | 03:34 | |
| -!- Aurelius_Home [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has joined ##hplusroadmap | 03:44 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-223-183.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 03:46 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-223-183.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 240 seconds] | 03:51 | |
| -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has joined ##hplusroadmap | 04:06 | |
| -!- augur [~augur@76-218-206-38.lightspeed.sntcca.sbcglobal.net] has quit [Ping timeout: 272 seconds] | 04:11 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has joined ##hplusroadmap | 04:12 | |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-nbegqbhixxmpqnhr] has quit [Quit: Connection closed for inactivity] | 04:22 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has quit [Ping timeout: 240 seconds] | 05:00 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 258 seconds] | 06:06 | |
| -!- augur [~augur@2602:304:cdac:e260:604e:5b19:842f:57db] has joined ##hplusroadmap | 06:07 | |
| -!- Aurelius_Work [~cpopell@209.48.69.2] has joined ##hplusroadmap | 06:07 | |
| -!- augur [~augur@2602:304:cdac:e260:604e:5b19:842f:57db] has quit [Ping timeout: 250 seconds] | 06:11 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-vmljbnehirggzula] has quit [Quit: Connection closed for inactivity] | 06:11 | |
| -!- zeroach [~CharlieNo@209.106.35.203] has joined ##hplusroadmap | 06:49 | |
| -!- bluebear_ [~chatzilla@80.95.97.194] has joined ##hplusroadmap | 06:51 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap | 06:57 | |
| -!- zeroach [~CharlieNo@209.106.35.203] has quit [Ping timeout: 276 seconds] | 06:57 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hvsgrazinicsfpmd] has joined ##hplusroadmap | 07:05 | |
| -!- jtimon [~quassel@55.31.134.37.dynamic.jazztel.es] has joined ##hplusroadmap | 07:10 | |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-qeuritzzxewhimho] has joined ##hplusroadmap | 08:25 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Remote host closed the connection] | 09:01 | |
| -!- zeroach [~CharlieNo@209.106.35.203] has joined ##hplusroadmap | 09:07 | |
| -!- nmz787_i [~ntmccork@134.134.139.76] has joined ##hplusroadmap | 09:09 | |
| kanzure | wyueejkdjnddbfh | 09:10 |
| -!- zeroach [~CharlieNo@209.106.35.203] has quit [Ping timeout: 260 seconds] | 09:18 | |
| kanzure | "Michael Greve today announced that his Forever Healthy Foundation will be committing $5 Million in philanthropic support over the next five years to the SENS Research Foundation" | 09:19 |
| kanzure | ""And we should have a whole rejuvenation industry based on the SENS treatment model including the self-accelerating feedback-loop of success stories and amazing opportunities for scientist, entrepreneurs and VC investors. This will truly accelerate both research and therapies. I have decided to lead by example and make this $10 million commitment,” said Michael Greve" | 09:19 |
| kanzure | 5 became 10 pretty fast | 09:19 |
| kanzure | "Forever Healthy Foundation’s initial donation will fund projects including Allotopic Expression of Mitochondrial Genes led by Dr. Matthew O’Connor at the SENS Research Foundation Research center and Pharmacological and/or enzymatic cleavage of glucosepane crosslinks led by Dr. David Spiegel at Yale University, SENS Research Foundation’s Education Program led by Dr. Greg Chin and other SENS Research Foundation programs" | 09:19 |
| kanzure | "Project|21 campaign to secure $50 million in private support from individual donors" | 09:20 |
| kanzure | https://www.reddit.com/r/Futurology/comments/4t65ay/aubrey_de_grey_ama_ask_about_the_quest_to_cure/ | 09:20 |
| CaptHindsight | only $10M! That's like only a few Kg of materials from Sigma :) | 09:27 |
| kanzure | yep that's how much they billed me when i called them to ask for their catalog | 09:28 |
| -!- augur [~augur@2602:304:cdac:e260:1d6c:5c28:a48c:2679] has joined ##hplusroadmap | 09:28 | |
| CaptHindsight | I'd like to see a project where buying from Sigma, Cole-Parmer, Thomas etc is banned | 09:28 |
| CaptHindsight | makes Neiman Marcus look like a thrift store | 09:29 |
| kanzure | CaptHindsight: in vivo dna polymerase is a good goal because you can avoid most reagents other than food. but v1 of any electronic polymerase will probably have to be in vitro (or more spcifically, probably in a nanopore of some kind). | 09:31 |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has joined ##hplusroadmap | 09:32 | |
| kanzure | CaptHindsight: but i think that long-term we can move biology work away from having lots of reagents, and into the set of amino acids provided naturally by cells | 09:32 |
| kanzure | delinquentme: i think your gchat thing is having sync issues again. i sent you the link. | 09:32 |
| -!- augur [~augur@2602:304:cdac:e260:1d6c:5c28:a48c:2679] has quit [Remote host closed the connection] | 09:41 | |
| kanzure | er, from yesterday, s/writeozyme/writozyme/ | 09:41 |
| kanzure | oh i was only grepping 200* | 09:41 |
| kanzure | apparently there were a few wiki pages. i should go tyr to recover those. | 09:42 |
| -!- bsm117532 [~mcelrath@38.121.165.30] has quit [Remote host closed the connection] | 09:46 | |
| -!- bsm117532 [~mcelrath@38.121.165.30] has joined ##hplusroadmap | 09:46 | |
| -!- bsm117532 [~mcelrath@38.121.165.30] has quit [Remote host closed the connection] | 09:47 | |
| -!- bsm117532 [~mcelrath@38.121.165.30] has joined ##hplusroadmap | 09:47 | |
| -!- ebowden_ [~ebowden@CPE-101-180-249-107.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 09:50 | |
| kanzure | "[http://heybryan.org/pipermail/synth2008/2008-June/000013.html [Synth2008] Fwd: [Hplusroadmap] [Synthetic Biology] Use a laser-inducable writozyme to make a plasmid, which then inserts genes]" | 09:52 |
| -!- zeroach [~CharlieNo@209.106.35.203] has joined ##hplusroadmap | 09:54 | |
| -!- ebowden_ [~ebowden@CPE-101-180-249-107.lnse3.cha.bigpond.net.au] has quit [Ping timeout: 260 seconds] | 09:54 | |
| -!- zeroach [~CharlieNo@209.106.35.203] has quit [Ping timeout: 244 seconds] | 10:04 | |
| -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-emtvnzohgkknnkbo] has quit [Ping timeout: 272 seconds] | 10:05 | |
| -!- btcdrak [uid165369@gateway/web/irccloud.com/x-kccfrqpobwcfhmhs] has quit [Ping timeout: 272 seconds] | 10:05 | |
| -!- blueskin [~blueskin@unaffiliated/blueskin] has quit [Ping timeout: 272 seconds] | 10:05 | |
| -!- nnnn20430 [nnnn@lightning.bouncer.ml] has quit [Ping timeout: 272 seconds] | 10:06 | |
| -!- nsh [~lol@wikipedia/nsh] has quit [Ping timeout: 272 seconds] | 10:06 | |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has quit [Quit: http://www.kiwiirc.com/ - A hand crafted IRC client] | 10:08 | |
| -!- augur [~augur@2602:304:cdac:e260:b434:9373:3884:e85c] has joined ##hplusroadmap | 10:08 | |
| -!- blueskin [~blueskin@2001:41d0:1:9185::1] has joined ##hplusroadmap | 10:10 | |
| -!- blueskin [~blueskin@2001:41d0:1:9185::1] has quit [Changing host] | 10:10 | |
| -!- blueskin [~blueskin@unaffiliated/blueskin] has joined ##hplusroadmap | 10:10 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has joined ##hplusroadmap | 10:11 | |
| -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-fbtxuustburppahj] has joined ##hplusroadmap | 10:12 | |
| -!- adamg [~akg@50.242.93.33] has joined ##hplusroadmap | 10:13 | |
| kanzure | "Ok, so the literature here and on the [[in vitro DNA synthesizer]] page show a way to pause polymerase via lasers and so on. The hard part is sending the molecular signal to polymerase to use a specific nucleotide type. Mike says this would be of the scale of a multi-lab, multi-PI effort, everybody's name on the paper." | 10:15 |
| -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-fbtxuustburppahj] has quit [Read error: Network is unreachable] | 10:20 | |
| -!- nsh [~lol@wikipedia/nsh] has joined ##hplusroadmap | 10:20 | |
| -!- Joshchamp [sid159459@gateway/web/irccloud.com/x-xiavzivyfpzyuvpf] has joined ##hplusroadmap | 10:22 | |
| -!- btcdrak [uid165369@gateway/web/irccloud.com/x-pembnhmtevagqsfy] has joined ##hplusroadmap | 10:22 | |
| -!- nnnn20430 [nnnn@2607:5300:60:96a4::9] has joined ##hplusroadmap | 10:24 | |
| kanzure | "Polyamine regulation of ribosome pausing" | 10:31 |
| kanzure | "A critical perspective on molecular electronic junctions: there is plenty of room in the middle" http://www.chem.ualberta.ca/~mccreery/RLM%20publication%20PDFs/mccreery229%20PCCP%20perspective.pdf (2013) | 10:36 |
| kanzure | "Electrochemical bioelectronic device consisting of metalloprotein for analog decision making" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4585857/ | 10:41 |
| -!- midnightmagic [~midnightm@unaffiliated/midnightmagic] has quit [Ping timeout: 264 seconds] | 10:46 | |
| -!- bluebear_ [~chatzilla@80.95.97.194] has quit [Quit: ChatZilla 0.9.92 [Firefox 47.0/20160606113944]] | 10:47 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 10:50 | |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has joined ##hplusroadmap | 10:55 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 10:55 | |
| -!- midnightmagic [~midnightm@unaffiliated/midnightmagic] has joined ##hplusroadmap | 10:56 | |
| -!- ArturSha1 [~ArturShai@37.218.162.107] has quit [Ping timeout: 276 seconds] | 11:08 | |
| -!- zer0ach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has joined ##hplusroadmap | 11:18 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has quit [Ping timeout: 240 seconds] | 11:20 | |
| kanzure | "The gamma complex of DNA polymerase III, composed of γδδ'χψ subunits, catalyzes ATP" | 11:23 |
| kanzure | -_- "γδδ'χψ subunits" | 11:23 |
| kanzure | .wik beta clamp | 11:27 |
| yoleaux | "A DNA clamp, also known as a sliding clamp, is a protein fold that serves as a processivity-promoting factor in DNA replication. As a critical component of the DNA polymerase III holoenzyme, the clamp protein binds DNA polymerase and prevents this enzyme from dissociating from the template DNA strand." — https://en.wikipedia.org/wiki/Beta_clamp | 11:27 |
| kanzure | .wik proliferating cell nuclear antigen | 11:27 |
| yoleaux | "Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication." — https://en.wikipedia.org/wiki/Proliferating_cell_nuclear_antigen | 11:27 |
| kanzure | "PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.[1]" | 11:27 |
| -!- zer0ach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has left ##hplusroadmap ["Leaving"] | 11:28 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 240 seconds] | 11:43 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 11:45 | |
| nmz787_i | "γδδ'χψ subunits" looks and sounds like an inner-city gang name and spray-paint "tag" | 11:48 |
| nmz787_i | as opposed to | 11:48 |
| nmz787_i | .wik g-unit | 11:48 |
| yoleaux | "G-Unit (short for Guerilla Unit) is an American hip hop group originating from South Jamaica, Queens, New York, formed by longtime friends and East Coast rappers 50 Cent, Tony Yayo and Lloyd Banks." — https://en.wikipedia.org/wiki/G-unit | 11:48 |
| nmz787_i | yo yo yo, γδδ'χψ subunits up in the hiz-ouse | 11:49 |
| nmz787_i | i don't even know how to pronounce that | 11:49 |
| nmz787_i | gamma something something something something something | 11:49 |
| kanzure | .u γ | 11:50 |
| yoleaux | U+03B3 GREEK SMALL LETTER GAMMA [Ll] (γ) | 11:50 |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 11:50 | |
| kanzure | .u δ | 11:50 |
| yoleaux | U+03B4 GREEK SMALL LETTER DELTA [Ll] (δ) | 11:50 |
| kanzure | .u χ | 11:50 |
| yoleaux | U+03C7 GREEK SMALL LETTER CHI [Ll] (χ) | 11:50 |
| kanzure | .u ψ | 11:50 |
| yoleaux | U+03C8 GREEK SMALL LETTER PSI [Ll] (ψ) | 11:50 |
| kanzure | gamma delta delta prime chi psi | 11:50 |
| -!- nmz787_i [~ntmccork@134.134.139.76] has quit [Quit: Leaving.] | 11:54 | |
| -!- nmz787_i [~ntmccork@134.134.139.76] has joined ##hplusroadmap | 11:55 | |
| nmz787_i | "Fine ZnS powder is an efficient photocatalyst, which produces hydrogen gas from water upon illumination." | 12:24 |
| nmz787_i | hmm, is this stuff expensive or... | 12:25 |
| nmz787_i | /me wonders about solar hydrogenation of CO2 | 12:25 |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has quit [Quit: http://www.kiwiirc.com/ - A hand crafted IRC client] | 12:30 | |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has joined ##hplusroadmap | 12:48 | |
| TMA | nmz787_i: $198 or 1072 € per 25 gram -- $7920 || 33075 € per kilo -- pretty expensive (€ from sigma aldritch, $ from US Nano) | 13:02 |
| TMA | the $ figure might be in grains not grams though | 13:04 |
| nmz787_i | wait, the euro is down by 5x ? | 13:04 |
| TMA | nmz787_i: more likely sigma aldritch is greedy | 13:05 |
| nmz787_i | hmm, google tells me 1 dollar is .9 euros | 13:05 |
| nmz787_i | there's a company in Oregon I contacted | 13:06 |
| TMA | from the paper it looks like not every ZnS is suitable | 13:18 |
| nmz787_i | oh, I contacted the company about a phosphor, not for photocatalysis | 13:18 |
| nmz787_i | (still ZnS) | 13:18 |
| TMA | it needs to be finely particulated AND have S vacancies | 13:19 |
| nmz787_i | those vacanies might be common though, I saw them earlier when I was looking for phosphor stuff | 13:20 |
| nmz787_i | (i saw mention of them) | 13:20 |
| nmz787_i | hmm, maybe I just saw the word on the wiki page | 13:22 |
| nmz787_i | can't find it in the PDFs I had open | 13:22 |
| TMA | I wonder whether the same process could be used to produce D2O as a byproduct (H2 escaping, while leaving the deuterium atoms in the liquid fraction) | 13:24 |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap | 13:30 | |
| nmz787_i | seems plausible | 13:33 |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has quit [Quit: http://www.kiwiirc.com/ - A hand crafted IRC client] | 13:41 | |
| -!- ielo [~ielo@88-106-250-129.dynamic.dsl.as9105.com] has joined ##hplusroadmap | 13:42 | |
| -!- ielo [~ielo@88-106-250-129.dynamic.dsl.as9105.com] has quit [Quit: Leaving] | 13:47 | |
| kanzure | http://www.coindesk.com/no-scaling-agreements-industry-bitcoin-meetup/ | 13:55 |
| -!- Regex__ [~Cara@2601:1c0:8501:d159:510b:d746:751f:b86c] has joined ##hplusroadmap | 14:11 | |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:75e9:8f09:4ab7:7c46] has quit [Ping timeout: 258 seconds] | 14:14 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-hvsgrazinicsfpmd] has quit [Quit: Connection closed for inactivity] | 14:21 | |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:510b:d746:751f:b86c] has joined ##hplusroadmap | 14:24 | |
| -!- augur [~augur@2602:304:cdac:e260:b434:9373:3884:e85c] has quit [Remote host closed the connection] | 14:24 | |
| -!- Regex__ [~Cara@2601:1c0:8501:d159:510b:d746:751f:b86c] has quit [Ping timeout: 250 seconds] | 14:28 | |
| kanzure | "step file analyzer" source code is now available on github http://www.nist.gov/el/msid/infotest/step-file-analyzer.cfm https://github.com/usnistgov/SFA | 14:29 |
| -!- Aurelius_Work [~cpopell@209.48.69.2] has quit [Ping timeout: 240 seconds] | 14:32 | |
| -!- jaboja [~jaboja@vps.jaboja.pl] has joined ##hplusroadmap | 14:36 | |
| chris_99 | Does anyone know if i'm right in thinking you could only identify things like diamonds and gemstones | 14:43 |
| chris_99 | with a far IR sensor | 14:43 |
| chris_99 | with spectroscopy | 14:43 |
| chris_99 | oh just found http://www.lotusgemology.com/index.php/library/articles/282-the-hand-spectroscope-for-testing-ruby-sapphire | 14:45 |
| -!- maaku [~quassel@173-228-107-141.dsl.static.fusionbroadband.com] has quit [Read error: Connection reset by peer] | 15:12 | |
| -!- maaku [~quassel@173-228-107-141.dsl.static.fusionbroadband.com] has joined ##hplusroadmap | 15:13 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has quit [Remote host closed the connection] | 15:22 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap | 15:23 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 15:30 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 15:35 | |
| -!- augur [~augur@2602:304:cdac:e260:906f:4671:9ffa:654e] has joined ##hplusroadmap | 15:42 | |
| -!- nmz787_i [~ntmccork@134.134.139.76] has left ##hplusroadmap [] | 16:01 | |
| -!- augur [~augur@2602:304:cdac:e260:906f:4671:9ffa:654e] has quit [Remote host closed the connection] | 16:01 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 16:25 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 16:29 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 276 seconds] | 16:36 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap | 16:44 | |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap | 17:00 | |
| chris_99 | https://www.reddit.com/r/IAmA/comments/4tgsaz/iama_i_built_a_fusion_reactor_in_my_bedroom_ama/ | 17:03 |
| kanzure | "Theoretical optimization method of buffer ionic concentration for protein detection using field effect transistors" http://jes.ecsdl.org/content/157/12/J410.short | 17:08 |
| -!- fleshtheworld [~fleshthew@108-240-244-194.lightspeed.frsnca.sbcglobal.net] has joined ##hplusroadmap | 17:16 | |
| kanzure | some patents about electrical conductance of polymerase for dna sequencing https://www.google.com/patents/US20150017655 and https://www.google.com/patents/US20150065353 (first one is the same group as the questionable paper from yesterday, second one is fro pacbio) | 17:19 |
| kanzure | *from pacbio | 17:19 |
| kanzure | "In some cases the substrate is exposed to four types of nucleotide analogs corresponding to A, G, C, T, or A, G, C, U, each of the four types of nucleotide analogs having a different impedance label. In some cases the impedance label is attached to the polyphosphate portion through a linker. In some cases the impedance label comprises either a capacitance label or a conductivity label." | 17:20 |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] | 17:21 | |
| kanzure | "proximate to the nanoscale electrode; exposing the polymerase to a plurality of types of nucleotide analogs, each comprising a different capacitive label attached to the phosphate portion of the nucleotide analog under conditions whereby polymerase mediated nucleic acid synthesis occurs, resulting in cleavage of the capacitive label and the growth of a nascent nucleic acid strand; applying electrical signals comprising alternating ... | 17:23 |
| kanzure | ... current over time to the nanoscale electrode, whereby when a nucleotide analog resides in the active site of the enzyme, the capacitive label on the nucleotide analog produces a measurable change in the capacitance at the nanoscale electrodes; monitoring the electrical signal at the nanoscale electrode over time, whereby the electrical signal indicates an incorporation event for a type of nucleotide analog having a specific ... | 17:23 |
| kanzure | ... capacitive label; and using the monitored electrical signal at the electrode over time to determine a sequence of the template nucleic acid." | 17:23 |
| kanzure | "In some embodiments the nanoscale electrode is repeatedly addressed at different frequencies, whereby the capacitance measured at each frequency is used to identify a specific capacitive label. In some embodiments the polymerase is exposed to four types of nucleotide analogs corresponding to A, G, C, T, or A, G, C, U, wherein the frequency of the nanoscale electrode is repeatedly addressed at least 8 different frequencies. In some ... | 17:24 |
| kanzure | ... embodiments the electrical signals applied to the electrode comprise sine waves, triangular waves, or saw tooth waves." | 17:24 |
| kanzure | "In some embodiments an amount of change in capacitance over time is used to identify which type of nucleotide is incorporated. In some embodiments the characteristics of capacitance versus frequency is used to identify which type of nucleotide is incorporated." | 17:25 |
| kanzure | "In some embodiments the frequency of the alternating current at the nanoscale electrodes is repeatedly brought to different frequency levels, whereby a characteristic capacitance versus frequency profile is used to identify a specific capacitive label." | 17:26 |
| kanzure | "the electrical signal used to determine the sequence of the template nucleic acids includes the duration of the signal indicating the residence time of a nucleotide analog in the active site of a polymerase" | 17:27 |
| kanzure | "when nucleotide analog is incorporated into the growing strand, the enzyme cleaves the polyphosphate portion of the nucleotide analog." | 17:29 |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has quit [Read error: Connection reset by peer] | 17:29 | |
| -!- justanotheruser [~Justan@unaffiliated/justanotheruser] has joined ##hplusroadmap | 17:30 | |
| kanzure | "In order for a redox based method to sense a label, one or more electrons must be exchanged between the label and the electrode, resulting in reduction or oxidation of the label. These types of reactions can produce products or intermediates such as radicals, radical anions, or radical cations that are can be reactive and unstable." | 17:32 |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] | 17:38 | |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap | 17:39 | |
| kanzure | " E. coli has a genome of roughly 5 million bp (BNID 100269). Replication rates are observed to be several hundred bp/sec (BNID 104120, 109251). Further, replication in these bacteria takes place with two replication forks heading in opposite directions around the circular bacterial chromosome. As shown in Figure 2, the replication rates imply that it should take the two replisomes at least 2500 sec (≈40 minutes) to replicate the ... | 17:39 |
| kanzure | ... genome, a number that is much longer than the minimal division time of ≈20 minutes (BNID 103514). This interesting estimate delivers a paradox that is resolved by the observation that E. coli under ideal growth conditions employs nested replication forks like those seen in Figure 2 that begin replicating the granddaughter and grand granddaughter cell’s genomes while the daughter cells are still themselves engaged in replication. ... | 17:39 |
| kanzure | ... At fast growth rates more than 6 origins of replication and over 10 replication forks coexist in a single cell (BNID 102356)" | 17:39 |
| kanzure | "The number of origins leading to replication [in eukaryotes] .. estimated .. for example in mouse they range from as low as 1,000 to as many as 100,000, while for Drosophila the estimate is about 10,000 (BNID 107654, 109283)." | 17:40 |
| kanzure | http://bitesizebio.com/392/the-best-polymerases-of-2008/ | 17:41 |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Ping timeout: 252 seconds] | 17:44 | |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has joined ##hplusroadmap | 17:55 | |
| -!- Dumuzi [Dumuzi@gateway/shell/panicbnc/x-mwcagwhxzkxzslbg] has joined ##hplusroadmap | 18:05 | |
| kanzure | "pseudo DC (where the measurement is essentially performed with a DC measurement, but the polarity is alternated to prevent corrosion)," | 18:08 |
| kanzure | "Conductivity labels are typically charged species that are water soluble. The conductivity labels can have multiple charges, e.g. from about 2 to about 2,000 charges. The labels can comprise dendrimers or nanoparticles. Multiple labels can be employed, each having a different level of charge, in some cases, with some labels positively charged and some labels negatively charged." | 18:08 |
| -!- hazirafel [~hazirafel@bzq-79-183-46-171.red.bezeqint.net] has joined ##hplusroadmap | 18:09 | |
| -!- ebowden_ [~ebowden@CPE-101-180-249-107.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 18:10 | |
| kanzure | "Polymerase enzyme substrates with protein shield" https://patents.google.com/patent/US20130316912A1 | 18:12 |
| kanzure | "Nucleotide analogs comprising conductivity labels will typically be larger, i.e. have a larger molecular weight than natural nucleotides. These analogs can include, for example, nucleotide analogs describe in U.S. patent application Ser. No. 13/767,619 entitled Polymerase Enzyme Substrates with Protein Shield, filed Feb. 14, 2013, and in U.S. Patent Application 61/862,502, entitled Protected Fluorescent Reagent Compounds, which are ... | 18:12 |
| kanzure | ... incorporated herein by reference for all purposes." | 18:12 |
| kanzure | can't find that second patent at the moment. might not be released. | 18:12 |
| kanzure | "In some cases the conductivity labels comprise beads, for example beads comprising multiple nucleotides attached via their polyphosphate portion. Such analogs are described, for example in U.S. Pat. No. 8,367,813 which is incorporated by reference herein in its entirety for all purposes. The beads can be coated with charged functional groups, anionic, cationic, or a combination of anionic and cationic groups. The amount of charge on the ... | 18:13 |
| kanzure | ... bead can be controlled in order to control the electrical signal at the gate of the nanoFET. The beads can have any usable size range, for example, between about 2 nm and about 50 nm in size. The shapes of the beads can be spherical, elongated, or other effective shape for controlling the current at the gate of the nanoFET" | 18:13 |
| -!- ebowden_ [~ebowden@CPE-101-180-249-107.lnse3.cha.bigpond.net.au] has quit [Ping timeout: 260 seconds] | 18:15 | |
| kanzure | "The size of the nanoparticle can influence the capacitance of the particle, as well as the chemical structure. Nanoparticles of metals, seimconductors, glasses, oxides, carbon, silicon, protein, polymers, ionic materials, can be used and can be produced to have widely different impedance magnitude and impedance versus frequency characteristics. However, it is to be understood that the impedance that is being measured is that of the ... | 18:17 |
| kanzure | ... region around the electrode, and not just that of the label. For example, a nanoparticle label will displace the solution near the electrode, such that the measured impedance will include that change. Thus, a capacitive label near the electrode can result in the impedance either going up or going down as compared to the impedance when the label is not present. | 18:17 |
| kanzure | " | 18:17 |
| kanzure | "Molecular adaptors for dye conjugates" https://patents.google.com/patent/US20120058473A1/ | 18:18 |
| kanzure | "Scaffold-based polymerase enzyme substrates" https://patents.google.com/patent/US20120077189A1/ | 18:19 |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:510b:d746:751f:b86c] has quit [Read error: Connection reset by peer] | 18:22 | |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:510b:d746:751f:b86c] has joined ##hplusroadmap | 18:23 | |
| kanzure | "Recombinant polymerases with increased phototolerance" https://patents.google.com/patent/US8906660B2/ | 18:27 |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has joined ##hplusroadmap | 18:31 | |
| -!- jaboja [~jaboja@vps.jaboja.pl] has quit [Remote host closed the connection] | 18:38 | |
| kanzure | attaching a physical bead to a nucleotide is a good idea. we should do that. | 18:45 |
| -!- Filosofem is now known as Jawmare | 18:48 | |
| -!- Aurelius_Work [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has joined ##hplusroadmap | 19:01 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 19:04 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 19:09 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 276 seconds] | 19:12 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap | 19:14 | |
| -!- jtimon [~quassel@55.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 252 seconds] | 19:17 | |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has quit [Ping timeout: 260 seconds] | 19:40 | |
| kanzure | .wik nanoputian | 19:41 |
| yoleaux | "NanoPutians are a series of organic molecules whose structural formulae resemble human forms. James Tour et al. (Rice University) designed and synthesized these compounds in 2003 as a part of a sequence on chemical education for young students." — https://en.wikipedia.org/wiki/Nanoputian | 19:41 |
| kanzure | "Nose grease can be used to minimize scratches in optical surfaces, for example when cleaning photographic negatives.[1] Observatory lore holds that nose grease was used to reduce stray light and reflections in transmissive telescopes before the development of vacuum antireflective coatings.[2] The antireflective properties are due in part to the fact that the nose oil fills small cracks and scratches and forms a smooth, polished ... | 19:48 |
| kanzure | ... surface, and in part to the low index of refraction of the oil, which can reduce surface reflection from transmissive optics that have a high index of refraction. " | 19:48 |
| kanzure | .wik self-inflicted caesarean section | 19:50 |
| yoleaux | "A self-performed caesarean section is a form of self-surgery where a woman attempts to perform a cesarean section on herself." — https://en.wikipedia.org/wiki/Self-inflicted_caesarean_section | 19:50 |
| -!- Malvolio [~Malvolio@unaffiliated/malvolio] has joined ##hplusroadmap | 19:54 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap | 20:19 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has joined ##hplusroadmap | 20:20 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e5e5:e86a:738:623b] has quit [Ping timeout: 260 seconds] | 20:25 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e0ac:b596:25a3:b6e7] has joined ##hplusroadmap | 20:29 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has quit [Ping timeout: 260 seconds] | 20:53 | |
| -!- HEx1 [~HEx@hexwab.plus.com] has joined ##hplusroadmap | 20:54 | |
| maaku | "She did say, afterward, that she didn't advise other women to follow her example" | 21:08 |
| maaku | that's an understatement | 21:08 |
| ebowden_ | Who's this? | 21:14 |
| ebowden_ | Oh, is it that woman who performed a c-section on herself? | 21:21 |
| -!- Aurelius_Work [~cpopell@c-73-200-185-48.hsd1.va.comcast.net] has quit [Ping timeout: 272 seconds] | 21:25 | |
| kanzure | i wonder what the thermodynamic limits of polymerase and polymerization are supposed to be | 21:31 |
| kanzure | and why aren't cells an implementation of grey goo? | 21:31 |
| kanzure | genome replication seems to be an important bottleneck | 21:31 |
| kanzure | it's probably something trivial like "there's no mutations that can trivially get modern polymerases towards some other location in fitness landscape to be any particulary higher performance" | 21:32 |
| -!- augur [~augur@noisebridge130.static.monkeybrains.net] has quit [Remote host closed the connection] | 21:34 | |
| kanzure | the stuff about multiple replication forks is actually disappointing | 21:35 |
| ebowden_ | What's so disapointing about multiple replication forks? | 21:39 |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-qeuritzzxewhimho] has quit [Quit: Connection closed for inactivity] | 21:42 | |
| ebowden_ | kanzure, ^ | 21:47 |
| kanzure | it means the perforance of an individual polymerase enzyme is too low to support whole genome replication in a timely way | 21:56 |
| ebowden_ | I wonder if faster, yet still high fidelity polymerases could be engineered. | 22:00 |
| -!- ArturSha1 [~ArturShai@37.218.162.107] has joined ##hplusroadmap | 22:13 | |
| -!- augur [~augur@2601:645:c100:63f1:291d:3d8f:a53:97b3] has joined ##hplusroadmap | 22:26 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:e0ac:b596:25a3:b6e7] has quit [Remote host closed the connection] | 22:37 | |
| -!- ebowden_ [~ebowden@2001:8003:100e:c500:2468:9f3b:3509:2100] has joined ##hplusroadmap | 22:47 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] | 22:52 | |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has joined ##hplusroadmap | 23:05 | |
| CaptHindsight | http://www.nature.com/nnano/journal/vaop/ncurrent/full/nnano.2016.131.html now we just need chlorine and copper based based biology vs carbon :) | 23:14 |
| -!- delinquentme [6ceb7099@gateway/web/cgi-irc/kiwiirc.com/ip.108.235.112.153] has quit [Quit: http://www.kiwiirc.com/ - A hand crafted IRC client] | 23:16 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has quit [Ping timeout: 250 seconds] | 23:23 | |
| -!- fleshtheworld [~fleshthew@108-240-244-194.lightspeed.frsnca.sbcglobal.net] has quit [Read error: Connection reset by peer] | 23:53 | |
| -!- zeroach [~CharlieNo@97-85-242-17.static.stls.mo.charter.com] has joined ##hplusroadmap | 23:54 | |
| --- Log closed Tue Jul 19 00:00:01 2016 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!