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| chris_99 | Anyone know of a database of visibile/IR spectrums, for assorted chemicals/materials etc | 01:51 |
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| nmz787 | chris_99: might try CRC manual | 03:31 |
| nmz787 | chris_99: or torrents or something :/ | 03:31 |
| ebowden_ | nmz787, any idea how to contact authors of a paper? | 03:31 |
| ebowden_ | The relevant paper is in future med chem. | 03:33 |
| chris_99 | nmz787, CRC manual? | 03:40 |
| ebowden_ | chris_99, have you any idea how I might contact the author of a paper? | 03:40 |
| chris_99 | this - http://www.hbcpnetbase.com/ ? | 03:40 |
| chris_99 | ebowden_, do they not list the email address on their paper | 03:40 |
| ebowden_ | Oh fucking derp, I didn't see the author for correspondence email address. | 03:44 |
| chris_99 | heh | 03:46 |
| ebowden_ | God damn it. | 03:54 |
| chris_99 | https://en.wikipedia.org/wiki/Heavy_water#Production just reading that, i wonder how hard it is to extract with electrolysis | 04:09 |
| TMA | chris_99: it's not very energy efficient in the first phases, when the deuterium contents is very low | 04:11 |
| chris_99 | ahh | 04:11 |
| chris_99 | could you do it though, given enough time, from tap water | 04:11 |
| TMA | chris_99: you most certainly can. | 04:12 |
| chris_99 | neat | 04:13 |
| TMA | chris_99: be prepared to pay huge electricity bills though | 04:15 |
| chris_99 | heh yeah | 04:16 |
| TMA | chris_99: (you might save a bit if you burn the resulting hydrogen and use the heat in a distillation step) | 04:16 |
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| chris_99 | i need to read about how the electrolysis works, as normally you get H and O right, so what happens to the D, how is that actually seperated | 04:18 |
| TMA | chris_99: when you split water into hydrogen and oxygen, the splitting occurs first with 1H (because it is lighter) the remaining water contains more 2H = D than the water you have put in | 04:21 |
| chris_99 | ah interesting | 04:23 |
| TMA | chris_99: so after you reduce the volume of the input water to some fraction, the liquid fraction is more concentrated than the input; lather rinse repeat :) | 04:23 |
| chris_99 | heh | 04:23 |
| chris_99 | how do you measure the % of H2O to D2O | 04:23 |
| Jenda` | maybe by density, D2O has about 1100 kg/m3 | 04:24 |
| Jenda` | mass spectrometer would be more precise, but most people don't have it at home | 04:24 |
| chris_99 | mm, density sounds a good waay | 04:25 |
| TMA | chris_99: it is difficult to measure precisely in the early phases | 04:28 |
| chris_99 | ah makes sense | 04:28 |
| chris_99 | with D2 is the same amount of energy released as H2, during combustion out of interest | 04:29 |
| TMA | makes sense -- the chemical bond energy is the same | 04:31 |
| chris_99 | aha | 04:31 |
| TMA | even in the electrolysis you need the same amount of energy to dissociate the water molecule | 04:32 |
| chris_99 | so i'm slightly confused, does the D2 not get released at all during electrolysis | 04:32 |
| TMA | but due to the weight differential the dissociated H+ moves faster than dissociated D+ | 04:32 |
| TMA | therefore it is less likely to be recaptured by another molecule | 04:33 |
| TMA | chris_99: it is released. but the release of D is slower (hence you want the electrolysis to stop at the right moment) | 04:34 |
| chris_99 | ah | 04:34 |
| chris_99 | hmm does the D2O sink to the bottom of a pool of tap water? | 04:35 |
| TMA | chris_99: in tap water the H:D ratio is 6420:1 ; after a single run of the electrolysis it might be like 6400:1, the reduction is very slow especially in the initial phases | 04:36 |
| chris_99 | ah eek | 04:37 |
| TMA | chris_99: if in sufficient concentration, otherwise it is knocked upwards by brown motion | 04:37 |
| chris_99 | gotcha | 04:37 |
| TMA | there is a reason why D2O is about 1000 €/kg | 04:38 |
| chris_99 | heh | 04:38 |
| chris_99 | there isn't another way to create it, doing something to Hydrogen, to turn it into D? | 04:39 |
| Jenda` | I don't think a neutron capture can occur | 04:39 |
| TMA | chris_99: put it in a reactor, it will capture neutrons | 04:39 |
| chris_99 | interesting | 04:40 |
| Jenda` | Business plan: if cars on hydrogen become popular, collect leftovers from electrolysis | 04:40 |
| chris_99 | heh | 04:40 |
| Jenda` | TMA: I don't think reactors produce significant amount of deuterium | 04:40 |
| Jenda` | well, year, Temelín (local nuclear power plant) releases tritium, but I don't know if it's from hydrogen or if it's a fissile product | 04:41 |
| TMA | Jenda`: it is less economically viable, but they do | 04:41 |
| TMA | Jenda`: Temelin uses light water, the tritium is partly from neutron capture | 04:42 |
| Jenda` | hm, so the water in the primary circuit slowly turns into D2O and then to T2O? | 04:43 |
| TMA | Jenda`: exactly | 04:43 |
| Jenda` | well, but how do they routinely release it, when the primary circuit is closed (and contaminated)? | 04:44 |
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| TMA | Jenda`: T for nuclear weapons was made by irradiating D2O in heavy water in a breeder reactor | 04:45 |
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| Jenda` | ah, ok, they really clean and release water from primary circuit: https://www.cez.cz/cs/vyroba-elektriny/jaderna-energetika/jaderne-elektrarny-cez/ete/technologie-a-zabezpeceni/11.html | 04:45 |
| Jenda` | I thought that they fill the primary circuit once and never drain/refill it | 04:46 |
| Jenda` | chris_99: maybe electrolysing water from rivers near a nuclear power plant would be a better start than electrolysing regular tap water https://www.cez.cz/edee/content/img/energie-a-zivotni-prostredi/jaderna-energetika-elektrarny-cez-ete-technologie-a-zabezpeceni-tritium.gif | 04:48 |
| chris_99 | what's Bq/l mean? | 04:49 |
| chris_99 | oh becquerel | 04:50 |
| Jenda` | red line = activity during drought, yellow = average activity; x-axis: kilometers | 04:51 |
| chris_99 | interesting | 04:52 |
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| fenn | TMA: i'd imagine the methods used for uranium enrichment work just fine on H2 gas as well, namely laser enrichment and microfluidic single-turn centrifuge/separators i.e. https://commons.wikimedia.org/wiki/File:Aerodynamic_enrichment_nozzle.svg | 06:02 |
| TMA | fenn: it might work directly with steam | 06:06 |
| TMA | i.e. no need to prepare H2 HD D2 mixture beforehand | 06:06 |
| TMA | fenn: do you have any idea how to compute the best geometry? | 06:07 |
| fenn | "Separation of Isotopes by Laser Excitation (SILEX)" jeez no wonder i was confused about SELEX | 06:08 |
| fenn | sorry i don't know very much about it, i imagine it's pretty similar to a mass spectrometer | 06:09 |
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| fenn | without the magnetic field of course | 06:09 |
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| fenn | enrichment nozzles are really small, i discovered it while reading about high aspect ratio silicon etching processes for MEMS | 06:19 |
| fenn | i remember seeing a bottle of D20 in a university chemistry lab sitting on a shelf, it can't be that expensive | 06:21 |
| TMA | fenn: the diagram on wikipedia has 1 mm bar for size comparison. I gather that the radius and aperture shall be different for diferent isotope separation tasks | 06:22 |
| TMA | fenn: university labs are well stocked -- the only reason they are not broken into more often is that the market for the more expensive reagents is small and the burglars are stupid | 06:26 |
| xentrac | I think there are much easier ways to separate deuterium, including mere chemical reactions and crystallization | 06:28 |
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| fenn | ah D20 is used for proton NMR solvents which makes sense for a chemistry department | 06:32 |
| xentrac | ah, D₂O | 06:37 |
| xentrac | I was picturing icosahedra | 06:37 |
| ebowden_ | I really, really like tiny containers. I hope to see an NMR tube one day. | 06:37 |
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| fenn | "According to the WNA, there are currently 436 operable reactors around the world with ~380 GWe generating capacity. Another 67 reactors are under construction in 14 countries, including 24 in China, 6 in India, and 5 in the USA. A further 166 reactors are on order or planned and 322 reactors are proposed to be built." | 06:49 |
| fenn | wow i didn't realize the USA is building new reactors | 06:50 |
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| chris_99 | http://www.economist.com/news/science-and-technology/21702343-individual-atoms-offer-ultra-dense-information-storage-scientists-pave-way wonder if they could just use lots of heads, maybe they already do | 06:59 |
| chris_99 | http://phoenixnuclearlabs.com/products/ | 07:08 |
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| JayDugger | fenn, does that include military reactors in subs and carriers, or just civilian? | 07:38 |
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| kanzure | researchgate spams you every time someone reads any of your publications? ugh | 08:31 |
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| kanzure | "Possible existence of optical communication channels in the brain" http://arxiv.org/abs/1607.02969 | 09:16 |
| kanzure | "Given that many fundamental questions in neuroscience are still open, it seems pertinent to explore whether the brain might use other physical modalities than the ones that have been discovered so far. In particular it is well established that neurons can emit photons, which prompts the question whether these biophotons could serve as signals between neurons, in addition to the well-known electro-chemical signals. For such communication ... | 09:17 |
| kanzure | ... to be targeted, the photons would need to travel in waveguides. Here we show, based on detailed theoretical modeling, that myelinated axons could serve as photonic waveguides, taking into account realistic optical imperfections. We propose experiments, both \textit{in vivo} and \textit{in vitro}, to test our hypothesis. We discuss the implications of our results, including the question whether photons could mediate long-range quantum ... | 09:17 |
| kanzure | ... entanglement in the brain." | 09:17 |
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| kanzure | protein graph repository http://wjdi.bioinfo.uqam.ca/ they have converted all PDB proteins into graph data | 09:29 |
| kanzure | "PGR: A Graph Repository of Protein 3D-Structures" http://arxiv.org/abs/1604.00045 | 09:29 |
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| kanzure | "mammalian poly(A) polymerase" http://wjdi.bioinfo.uqam.ca/data/explore.html?graphId=113067 | 09:30 |
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| kanzure | "A theoretical model for template-free synthesis of long DNA sequence" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735642/ (2009) | 10:04 |
| kanzure | "This theoretical scheme is intended to formulate a potential method for high fidelity synthesis of Nucleic Acid molecules towards a few thousand bases using an enzyme system. Terminal Deoxyribonucleotidyl Transferase, which adds a nucleotide to the 3′OH end of a Nucleic Acid molecule, may be used in combination with a controlled method for nucleotide addition and degradation, to synthesize a predefined Nucleic Acid sequence. A pH ... | 10:04 |
| kanzure | ... control system is suggested to regulate the sequential activity switching of different enzymes in the synthetic scheme. Current practice of synthetic biology is cumbersome, expensive and often error prone owing to the dependence on the ligation of short oligonucleotides to fabricate functional genetic parts. The projected scheme is likely to render synthetic genomics appreciably convenient and economic by providing longer DNA ... | 10:04 |
| kanzure | ... molecules to start with." | 10:04 |
| kanzure | the "molecular assemblies, inc." patent references that paper https://www.google.com/patents/US9279149 | 10:11 |
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| kanzure | "(It is estimated that the annual demand for oligonucleotide synthesis is responsible for greater than 300,000 gallons of hazardous chemical waste, including acetonitrile, trichloroacetic acid, toluene, tetrahydrofuran, and pyridine. See LeProust et al., Nucleic Acids Res., vol. 38(8), p. 2522-2540, (2010), " | 10:20 |
| kanzure | "Another embodiment for using non-template dependent polymerase/transferase enzymes would be to using protein engineering or protein evolution to modify the enzyme to remain tightly bound and inactive to the nascent strand after each single nucleotide incorporation, thus preventing any subsequent incorporation until such time as the polymerase/transferase is released from the strand by use of a releasing reagent/condition. Such ... | 10:22 |
| kanzure | ... modifications would be selected to allow the use of natural unmodified dNTPs instead of reversible terminator dNTPs. Releasing reagents could be high salt buffers, denaturants, etc. Releasing conditions could be high temperature, agitation, etc. For instance, mutations to the Loop1 and SD1 regions of TdT have been shown to dramatically alter the activity from a template-independent activity to more of a template dependent activity. ... | 10:22 |
| kanzure | ... Specific mutations of interest include but are not limited to Δ3384/391/392, del loop1 (386→398), L398A, D339A, F401A, and Q402K403C404→E402R403S404. Other means of accomplishing the goal of a post-incorporation tight binding (i.e., single turnover) TdT enzyme could include mutations to the residues responsible for binding the three phosphates of the initiator strand including but not limited to K261, R432, and R454." | 10:23 |
| kanzure | hm | 10:28 |
| kanzure | you could have capactive labels on nucleotides that have a certain electrical signal in response to optical illumination, e.g. to make a really strong signal for electrical detection | 10:29 |
| kanzure | or making a really specific signature i mean | 10:29 |
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| FourFire | Aubrey is having an AMA now | 10:39 |
| FourFire | Anyone care to criticise my questions for him: https://www.reddit.com/r/Futurology/comments/4t65ay/aubrey_de_grey_ama_ask_about_the_quest_to_cure/d5icja5 | 10:40 |
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| kanzure | "To date, CRISPR Therapeutics has raised $154 million through the sale of equity. The company banked another $75 million last October from Boston’s Vertex Pharmaceuticals, which licensed the rights to as many as six CRISPR-based therapies and promised to pay as much as $420 million for each down the road." | 11:37 |
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| kanzure | "Techniques used to study the DNA polymerase reaction pathway" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846202/ | 11:47 |
| kanzure | "Despite the focus of fluorescence studies on the prechemistry steps of the DNA polymerase mechanism, the nature of the slow step that is rate-limiting for dNTP incorporation remains a mystery. In some cases, as described earlier for T7 DNA polymerase, it is possible that a more detailed analysis of the reaction pathway will reveal that the chemical step itself is rate-limiting [18]. In others, exemplified by Klenow fragment, the ... | 11:47 |
| kanzure | ... rate-limiting step may involve relatively subtle structural changes and consequently be fluorescently silent with the probes that have been used. A stopped-flow fluorescence experiment, in which Ca2+ was used instead of Mg2+, suggests that the rate-limiting prechemistry step of Klenow fragment could involve the entry into the active site of the metal ion that activates the primer 3′OH [29]. Likewise, it has been suggested that ... | 11:47 |
| kanzure | ... entry of the second catalytic metal ion could take place immediately before chemistry in the DNA polymerase β mechanism [22, 23]." | 11:47 |
| kanzure | figure 8 and figure 9 are diagrams of the chemical reaction pathway provided by dna polymerase | 11:50 |
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| kanzure | .wik scanning ion conductance microscopy | 11:58 |
| yoleaux | "Scanning ion-conductance microscopy (SICM) is a scanning probe microscopy technique that uses an electrode as the probe tip. SICM allows for the determination of the surface topography of micrometer and even nanometer-range structures in aqueous media conducting electrolytes." — https://en.wikipedia.org/wiki/Scanning_ion-conductance_microscopy | 11:58 |
| kanzure | .wik scanning electrochemical microscopy | 11:58 |
| yoleaux | "Scanning electrochemical microscopy (SECM) is a technique within the broader class of scanning probe microscopy (SPM) that is used to measure the local electrochemical behavior of liquid/solid, liquid/gas and liquid/liquid interfaces. Initial characterization of the technique was credited to University of Texas electrochemist, Allen J." — https://en.wikipedia.org/wiki/Scanning_electrochemical_microscopy | 11:58 |
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| kanzure | "Role of conformational motions in enzyme function - Selected methodologies and case studies" http://www.mdpi.com/2073-4344/6/6/81/pdf | 12:01 |
| kanzure | "DNA polymerase conformational dynamics and the role of fidelity-conferring residues: Insights from computational simulations" http://journal.frontiersin.org/article/10.3389/fmolb.2016.00020/full | 12:04 |
| kanzure | "We report a comprehensive atomic resolution study of wild type and mutant enzymes in different bound states and starting from different crystal structures, using extensive molecular dynamics (MD) simulations that cover a total timespan of ~5 ms. [...] Our results show that the presence of fidelity-decreasing mutations or the binding of incorrect nucleotides in ternary complexes tend to favor transitions from closed toward open ... | 12:04 |
| kanzure | ... structures, passing through an ensemble of semi-closed intermediates." | 12:04 |
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| kanzure | "Molecular events during translocation and proofreading extracted from 200 static structures of DNA polymerase" http://nar.oxfordjournals.org/content/early/2016/06/20/nar.gkw555.full | 12:14 |
| kanzure | "This enzyme catalyzes incorporation of hundreds of nucleotides each second in host cells. That is to say, its fingers domain is wagging between the open and closed positions at a frequency of several hundred Hz during normal elongation (300 Hz is used in calculations throughout this paper). The fingers domain acts as an oscillator just like one in a mechanical clock and is responsible for power management of both translocation and ... | 12:15 |
| kanzure | ... processive active site switching. Once this oscillation is established, each leaving pyrophosphate (PPi) bound to the fingers in the closed conformation replenishes energy to maintain the oscillation. With each push from a newly cleaved PPi resulting from dNTP hydrolysis, the closed fingers accelerate to adopt an open conformation. At the end of a large swing, the fingers slam into the N-terminal domain. When the motion of the ... | 12:15 |
| kanzure | ... fingers is completely halted at the open position, their momentum is transferred to power the translocation. The motion of the thumb drives a back-and-forth displacement of the product duplex during translocation. The structural events during translocation and active site switching share much of the same conformational pathway until they branch out at an advanced stage." | 12:15 |
| kanzure | hmm we should email this guy | 12:16 |
| kanzure | zren@uic.edu | 12:16 |
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| chris_99 | https://thescribblepen.com/ -- wonder how that works, if it isn't a scam | 12:24 |
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| xentrac | chris_99: such systems have been commonplace in paint stores for decades, mixing at a liter scale rather than microliter | 12:29 |
| chris_99 | i can only think it could use some kind of microfludic chip maybe? | 12:30 |
| xentrac | is that the part that puzzles you? I think nanoliter-resolution pumps have been available for decades now | 12:31 |
| xentrac | for e.g. medical devices like implanted insulin pumps | 12:32 |
| chris_99 | well i guess you could use a piezo head | 12:32 |
| chris_99 | but to do all that in the space of a pen | 12:34 |
| xentrac | it's kind of a fat pen | 12:36 |
| chris_99 | true | 12:38 |
| chris_99 | http://www.dolomite-microfluidics.com/webshop/pumps-piezo-pumps-c-38_50/stainless-steel-piezoelectric-pump-p-231 that looks pretty small | 12:38 |
| CaptHindsight | another ghetto fluid handling project http://www.3ders.org/articles/20160714-ai-biosciences-repurposes-low-cost-printrbot-3d-printer-into-high-quality-bio-extraction-instrument.html | 12:48 |
| CaptHindsight | chris_99: CMYK inks + pumps | 12:55 |
| chris_99 | CaptHindsight, how would the mixing work | 12:55 |
| chris_99 | thoo | 12:55 |
| CaptHindsight | mix right before the tip | 12:55 |
| xentrac | yeah, mix in the tip | 12:56 |
| chris_99 | using microfluidic type stuff? | 12:56 |
| CaptHindsight | If I had one I could probably tell you how much before based on how long it takes to change colors | 12:56 |
| xentrac | sort of by definition, yeah, but it isn't really any more microfluidic than a regular ballpoint pen | 12:56 |
| xentrac | mixing becomes easier at microfluidic scales and regular human timescales because the distance across the cavity is small compared to the diffusion rate | 12:57 |
| CaptHindsight | I couldn't make it through the videos... | 12:57 |
| CaptHindsight | do they show what happens when you change from one color to the next? | 12:57 |
| CaptHindsight | do you need to blot the tip until the color changes? | 12:58 |
| chris_99 | conventiantly they don't show | 12:58 |
| chris_99 | that | 12:58 |
| CaptHindsight | Scribble's ink cartridge connects to a smart micro pump that recreates the color you have scanned. | 12:59 |
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| xentrac | you could do the blotting internally and robotically but they probably don't bother for the first release | 13:06 |
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| CaptHindsight | Scribble Gene Pen, change your the color and shape of your eyes, skin and hair easily in your own home | 13:17 |
| CaptHindsight | no more running to the Gene Salon | 13:19 |
| kanzure | unfortunately since it wont have an instantaneous effect, users will think it doesn't work | 13:39 |
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| nmz787_i | kanzure: that is probably the best biophoton paper I | 13:54 |
| nmz787_i | I've read* | 13:54 |
| nmz787_i | also the bangladeshi paper on pH control is pretty interesting | 13:54 |
| kanzure | pH control is unlikely to be real-timey enough though | 13:54 |
| nmz787_i | yeah, interesting since we actually mentioned that on call | 13:55 |
| nmz787_i | and in general their view was pretty formal | 13:55 |
| nmz787_i | (which is becoming more interesting to me as of late, getting into the SAT solver stuff) | 13:55 |
| kanzure | did you see the pacbio patent yesterday about capactive and inductive sensing of a polymerase enzyme? | 13:56 |
| nmz787_i | hrmm, not sure | 13:57 |
| kanzure | that sort of technique is probably going to require lots of signals sent over the CMOS traces to get an individual per-wire level view of what sort of noise is happening, otherwise you probably wont be able to see each individual base incorporation at any relevant level of detail | 13:57 |
| nmz787_i | luckily electrical traces aren't as hard for fabs as MEMS/fluidics seem to be | 14:00 |
| nmz787_i | I'll take a look | 14:01 |
| kanzure | nmz787_i: https://www.google.com/patents/US20150065353 pacbio dna sequencing by electrical conductance measurement of dna polymerase | 14:01 |
| kanzure | notes http://gnusha.org/logs/2016-07-18.log | 14:01 |
| nmz787_i | https://www.google.com/patents/US20050147979 | 14:01 |
| nmz787_i | not quite the one I am remembering | 14:02 |
| nmz787_i | but it seems they made a series of similar claims | 14:02 |
| nmz787_i | http://www.google.com/patents/US20040110208 | 14:03 |
| nmz787_i | that's it | 14:03 |
| nmz787_i | well they mention the FET on-chip, which is what I assumed would probably be needed... this complicates things from a "little guy prototyping" standpoint... but it might not be deadly (maybe there are some CSP, chip scale package, FETs available... etc... that could stand-in for a prototype) | 14:06 |
| xentrac | https://www.youtube.com/watch?v=Wex_yKfrTo4 AvE generates refractory carbon foam by charring bread | 14:19 |
| xentrac | white bread | 14:19 |
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| xentrac | hmm, video has some NSFW parts, if you care | 14:23 |
| xentrac | original paper http://phys.org/news/2016-07-multi-use-stiff-carbon-foam-bread.html | 14:25 |
| xentrac | well, OK, news article on original paper | 14:25 |
| xentrac | original paper http://pubs.acs.org/doi/abs/10.1021/acsami.6b03985 | 14:26 |
| xentrac | 3.6 MPa compressive strength, .29 g/cc, 121 MPa compressive modulus | 14:28 |
| xentrac | .06 W/m/K thermal conductivity | 14:28 |
| xentrac | wonder if you could do metal casting in it | 14:29 |
| xentrac | heh, "recently, carbon foams based on biomass (such as watermelon, lignin, bagasse, pomelo peel, banana peel, bacterial fiber, bacterial cellulose, et al) have been successfully fabricated by different methods" | 14:30 |
| kanzure | i suspect polymerase does not operate at higher frequency because the resource requirements would be too toxic to cells | 14:37 |
| nmz787_i | I wonder if it would get too hot | 14:37 |
| nmz787_i | or yeah be too hungry, and starve some other required-for-life part of the cell | 14:38 |
| kanzure | well that metal ion for example, is that used once per incorporation | 14:38 |
| kanzure | so lots of metal would be ungood.... for many reasons.. | 14:38 |
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| kanzure | "FRET is analogous to near-field communication, in that the radius of interaction is much smaller than the wavelength of light emitted. In the near-field region, the excited chromophore emits a virtual photon that is instantly absorbed by a receiving chromophore. These virtual photons are undetectable, since their existence violates the conservation of energy and momentum, and hence FRET is known as a radiationless mechanism" | 14:42 |
| kanzure | wat | 14:43 |
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| CaptHindsight | nmz787_i: making tools to make devices with a few gates at a few nm should not be a problem | 14:58 |
| CaptHindsight | tools for large devices with billions of gates is another story | 14:59 |
| CaptHindsight | the nanorap should do it, FDM on the nanoscale | 14:59 |
| CaptHindsight | easily deposits metal oxides on silicon with nm features | 15:00 |
| CaptHindsight | only the software will be buggy after a few uses and it will require constant realignment every print or two :) | 15:01 |
| xentrac | I've also prepared carbon foam by heating sugar in a spoon on a gas burner. My dad was a little mad because I never could get all the carbon off the spoon | 15:01 |
| xentrac | CaptHindsight: nanorap? | 15:01 |
| CaptHindsight | xentrac: a teeny tiny reprap for printing electronics | 15:02 |
| CaptHindsight | you could fit 100 across the width of your hair | 15:02 |
| CaptHindsight | now to work on how to get woodgrain shrunk down to the nanoscale? | 15:03 |
| xentrac | does it work? | 15:04 |
| xentrac | I'm skeptical about this idea of FDM on the nanoscale | 15:04 |
| CaptHindsight | how else will 100nm high Yoda heads be manufactured? | 15:06 |
| CaptHindsight | 2-photon polymerization? | 15:06 |
| xentrac | I will shit them out | 15:06 |
| xentrac | thoughts on the bread? | 15:07 |
| xentrac | having just watched a YouTube video of some Canadian roughneck melting aluminum castings with a torch on top of carbonized bread, I'm stoked | 15:07 |
| CaptHindsight | does it also work on Starbucks coffee? | 15:08 |
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| xentrac | what, like spent coffee grounds? if it works on bagasse, banana peel, and watermelon, it'll probably work on coffee grounds too | 15:10 |
| xentrac | not sure you'll have a very strong foam though | 15:11 |
| xentrac | maybe if you add a little bit of sugar as a binder | 15:11 |
| CaptHindsight | Starbucks sells coffee flavored carbon water | 15:13 |
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| nmz787_i | xentrac: FIB is essentially FDM | 15:32 |
| xentrac | heh, at a certain layer of abstraction, yes | 15:33 |
| xentrac | in the same way that bread is essentially a watermelon | 15:33 |
| ebowden_ | lol | 15:33 |
| nmz787_i | umm | 15:33 |
| ebowden_ | What is FIB? | 15:33 |
| xentrac | focused-ion-beam lithography | 15:33 |
| nmz787_i | I literally told someone yesterday that FIB is essentially a nano milling machine + 3d printer | 15:33 |
| xentrac | sure, I'll agree with that | 15:34 |
| nmz787_i | made no mention of bread and watermelon analogies | 15:34 |
| nmz787_i | no analogies whatsoever other than to a 3d printer | 15:34 |
| nmz787_i | (and milling machine) | 15:34 |
| xentrac | ebowden_: you accelerate a beam of ions with a high voltage and focus them to a point with a magnetic field, just as in an electron microscope, except using ions instead of electrons | 15:35 |
| nmz787_i | I /have/ in the past likened it to shooting a gun at a firing range, and when you want to deposit/3d-print... you make some ducks/small-birds fly across the gun range... and when they get shot the bullet is so energetic it pushes them to the target wall, and smashes them there, where they may accumulate | 15:35 |
| nmz787_i | xentrac: actually most ion optics use electrostatic lensing | 15:36 |
| xentrac | this allows you to implant the ions into the surface, stick them to the surface, or vaporize the surface, depending on the energy and how many of them there are | 15:36 |
| xentrac | oh! thanks! I didn't realize that | 15:36 |
| xentrac | SEMs usually use magnetic lensing, no? or am I confused about that too? | 15:36 |
| xentrac | I didn't know about the duck deposition either; I just figured you just directly accelerated ions of whatever you wanted to deposit | 15:38 |
| xentrac | the acceleration is just with a static electric field, right? or do you use a cyclotron or synchrotron approach to get higher energies on those fat, slow ions? | 15:39 |
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| nmz787_i | SEMs use magnetics usually | 15:42 |
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| nmz787_i | pretty sure it's just a static e-field... the voltage between the hot solder-like metal-source and the bottom of the column | 15:43 |
| nmz787_i | the duck analogy is a bit off... really the bullets would probably scatter, imparting its energy on the duck, and also burning it's feathers off so it couldn't fly away (vaporizing carbonyl tail usually) | 15:44 |
| nmz787_i | I think its a carbonyl | 15:46 |
| nmz787_i | hmm, suppliers show Trimethyl(methylcylopentadienyl)Platinum(IV) | 15:47 |
| nmz787_i | I can't remember the generic class of metal-carbon these fall in, or where I thought they did | 15:48 |
| CaptHindsight | have to make a MyFirst FIB Fab | 15:54 |
| CaptHindsight | make your own nanoscale devices, electronics, machines, Yoda Heads | 15:55 |
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| nmz787_i | in time, maybe | 15:58 |
| CaptHindsight | it's near the top of my list | 15:59 |
| CaptHindsight | new POSAM first | 15:59 |
| CaptHindsight | the ChinaCo Epson printers are too poorly made to be able use them | 16:01 |
| CaptHindsight | new droplet sorter http://phys.org/news/2016-07-surface-tension-droplets-biomedical-applications.html | 16:02 |
| CaptHindsight | with video | 16:02 |
| CaptHindsight | http://pubs.rsc.org/en/Content/ArticleLanding/2016/LC/C6LC00673F#!divAbstract | 16:03 |
| xentrac | CaptHindsight: to use them for what? | 16:03 |
| CaptHindsight | sort blue DNA from Green DNA for example | 16:04 |
| CaptHindsight | oligos in brine | 16:04 |
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| kanzure | hrmph | 18:02 |
| kanzure | we should have a library of standard conformational changes, caused by charge transform or something upon electrical stimulation or optical illumination. and then we need components that do 90 degree angles and other weird motions. | 18:04 |
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| SloanOnLinux | Hi there | 18:15 |
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| kanzure | SloanOnLinux: hi. | 18:18 |
| SloanOnLinux | This channel seems to be about things I'm interested in | 18:19 |
| SloanOnLinux | So yeah I'm new here, sup? | 18:20 |
| maaku | Welcome. | 18:38 |
| maaku | Maybe start with your interests and skills? | 18:38 |
| SloanOnLinux | I am interested in computers, politics, biohacking, open source, I have dabbled in python and reverse engineering. I came here to learn. | 18:46 |
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| nmz787 | SloanOnLinux: what kind of reverse engineering? computers, mechanical systems, electronics, dolphin brains (etc)? | 19:22 |
| SloanOnLinux | I took schtuph apart when I was a kid, then when I experienced internet for the first time I hacked a game with cheat engine | 19:24 |
| kanzure | SloanOnLinux: cheat engine is fun. here is a thing i did: https://github.com/kanzure/pokecrystal | 19:30 |
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| SloanOnLinux | kanzure: Cool, how long did it take for you to do that_ | 20:49 |
| kanzure | bunch of other people got involved, no idea | 20:51 |
| justanotheruser | greetings | 20:52 |
| SloanOnLinux | Oh | 20:52 |
| SloanOnLinux | Hi | 20:52 |
| justanotheruser | hi SloanOnLinux, what are you working on | 20:52 |
| SloanOnLinux | Nothing | 20:53 |
| SloanOnLinux | Hbu_ | 20:55 |
| SloanOnLinux | ?* | 20:55 |
| SloanOnLinux | Can I help with anything, justanotheruser? | 20:56 |
| kanzure | you could read logs and write summaries on the wiki, that would be really helpful http://gnusha.org/logs/ | 20:57 |
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| SloanOnLinux | Where do I submit these summaries | 21:08 |
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| kanzure | SloanOnLinux: our wiki http://diyhpl.us/wiki/ | 21:10 |
| kanzure | "repurposing the ribosome to do DNA synthesis" actually this might be possible, since the ribosome is a ribozyme, and there's a ribozyme polymerase..... | 21:28 |
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