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| aedla | I'm trying to find the part II of http://www.silva.bsse.ethz.ch/bpl/education/synthbiology10/SB_primer | 04:06 |
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| kanzure | i dunno if that exists. | 06:42 |
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| kanzure | "designer receptors exclusively activated by designer drugs (DREADDs)" | 08:30 |
| kanzure | "Instead of an engineered microbial opsin gene, the gene for an entire protein receptor for a designer drug—a compound, such as clozapine-N-oxide (CNO), which nowhere occurs naturally in biological tissue—is inserted via the virus vector. These engineered receptors are metabotropic (coupled to a G-protein), to induce either bursting (Gq-type protein) or silencing (Gi-type protein) in infected neurons. The designer drug is then ... | 08:35 |
| kanzure | ... administered systemically, either through peritoneal injections or diet. It binds to the engineered receptors in the infected neurons, activates the attached G-protein complex, and induces bursting or silencing in those neurons for the extent of the pharmacological activity of the designer drug." | 08:35 |
| kanzure | "Systemic administration of the designer drug means that all the neurons infected with and expressing the transgene for the designer receptor will be affected, no matter where they are located in the brain. In optogenetics, only those neurons infected, expressed, and in the range of the stimulating light source are affected. This extent of the drug’s effect is a two-edged sword, however. It puts a premium on micro-injection placement ... | 08:38 |
| kanzure | ... of the DREADD-containing virus and subsequent verification of this placement. Leakage of the virus into other non-target neurons, and subsequent expression of the designer receptor in them, can confound behavioral results. On the other hand, DREADDs’ temporal control is tied to the pharmacokinetics and -dynamics of the designer drug—its absorption, distribution, metabolism into inactive metabolites, and excretion. This slow and ... | 08:39 |
| kanzure | ... imprecise temporal effect contrasts sharply with optogenetics’ temporally much more tightly controlled on/off light switch." | 08:39 |
| kanzure | from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4782158/ | 08:39 |
| kanzure | "Liu et al. (2012) used a transgenic mouse line in which an excitatory opsin (ChR2) was expressed only during fear conditioning to the novel context, in the most active neurons in the dentate gyrus region of the hippocampus. Expression was limited by the tetracycline-controlled transcriptional activation system described above. ... Liu et al. (2012) ingenious experimental design was to reactivate the infected cells encoding the ... | 08:42 |
| kanzure | ... context-shock association to the first context while the animal was exploring a second and different novel context. No actual foot shock was administered in that second context. However, photostimulated experimental mice later demonstrated significant freezing to the second, non-shocked, context." | 08:42 |
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| kanzure | "Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs" http://www.nature.com/articles/ncomms12501 | 09:08 |
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| kanzure | "Behavioral and neuroanatomical investigation of Highly Superior Autobiographical Memory (HSAM)" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764458/ | 13:10 |
| kanzure | "A single case study recently documented one woman’s ability to recall accurately vast amounts of autobiographical information, spanning most of her lifetime, without the use of practiced mnemonics (Parker, Cahill, & McGaugh, 2006). The current study reports findings based on eleven participants expressing this same memory ability, now referred to as Highly Superior Autobiographical Memory (HSAM). Participants were identified and ... | 13:10 |
| kanzure | ... subsequently characterized based on screening for memory of public events. They were then tested for personal autobiographical memories as well as for memory assessed by laboratory memory tests. Additionally, whole-brain structural MRI scans were obtained. Results indicated that HSAM participants performed significantly better at recalling public as well as personal autobiographical events as well as the days and dates on which these ... | 13:10 |
| kanzure | ... events occurred. However, their performance was comparable to age- and sex-matched controls on most standard laboratory memory tests. Neuroanatomical results identified nine structures as being morphologically different from those of control participants. The study of HSAM may provide new insights into the neurobiology of autobiographical memory." | 13:10 |
| kanzure | "targeted memory reactivation (TMR) in sleep using audio cues" | 13:48 |
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| pasky | we've got an open beta at http://vize.it/ | 14:54 |
| pasky | feedback welcome | 14:54 |
| kanzure | feedback: skip doing images. there are better things to be tinkering on. | 14:55 |
| pasky | it's what is easy to do with good enough quality and makes direct money :) | 14:56 |
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| kanzure | .wik cell-penetrating peptides | 19:42 |
| yoleaux | "Cell-penetrating peptides (CPPs) are short peptides that facilitate cellular intake/uptake of various molecular equipment (from nanosize particles to small chemical molecules and large fragments of DNA). The "cargo" is associated with the peptides either through chemical linkage via covalent bonds or through non-covalent interactions." — https://en.wikipedia.org/wiki/Cell-penetrating_peptides | 19:42 |
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| kanzure | "Cognitive enhancement by transcranial laser stimulation and acute aerobic exercise" http://link.springer.com/article/10.1007%2Fs10103-016-1962-3 | 20:20 |
| kanzure | "... transcranial infrared laser stimulation of the prefrontal cortex .. " alright | 20:20 |
| kanzure | "low-level laser therapy (LLLT) with infrared laser to two forehead sites while seated (total 8 min, 1064 nm continuous wave, 250 mW/cm2, 60 J/cm2 per site of 13.6 cm2)" | 20:20 |
| kanzure | i wonder if anyone has bothered to attach a refrigerator to someone's head while doing any of these tests. | 20:21 |
| kanzure | and "Low-level light therapy improves cortical metabolic capacity and memory retention" http://content.iospress.com/openurl?genre=article&id=doi:10.3233/JAD-2012-120817 | 20:22 |
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| ybit | http://www.prnewswire.com/news-releases/molecular-biology-enzymes-and-kits--reagents-market-worth-1269-billion-usd-by-2021-592757341.html | 20:42 |
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| kanzure | "periodic limb movement disorder" | 21:04 |
| kanzure | why are there no optogenetics/sleep/memory consolidation studies. what. | 21:07 |
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