| --- Log opened Sat Nov 05 00:00:41 2016 | ||
| -!- ebowden_ [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 00:05 | |
| -!- esmerelda [~andares@unaffiliated/jacco] has joined ##hplusroadmap | 00:06 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:fd8e:34b1:9b42:639c] has joined ##hplusroadmap | 00:07 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:fd8e:34b1:9b42:639c] has quit [Changing host] | 00:07 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 00:07 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 00:08 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:7107:6b1b:68fa:84e3] has joined ##hplusroadmap | 00:20 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:7107:6b1b:68fa:84e3] has quit [Changing host] | 00:20 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 00:20 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 01:06 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 01:06 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 01:06 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 01:06 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 01:13 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:f934:ac25:8d55:1bbe] has joined ##hplusroadmap | 01:14 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:f934:ac25:8d55:1bbe] has quit [Changing host] | 01:14 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 01:14 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 01:16 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 01:16 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 01:16 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 01:16 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 01:30 | |
| -!- ufoinc [~hazirafel@bzq-109-67-17-9.red.bezeqint.net] has joined ##hplusroadmap | 01:53 | |
| -!- hazirafel [~hazirafel@bzq-79-179-127-5.red.bezeqint.net] has quit [Ping timeout: 244 seconds] | 01:54 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Remote host closed the connection] | 02:16 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 02:27 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:db8:d74e:5ba5:2a02] has joined ##hplusroadmap | 02:28 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:db8:d74e:5ba5:2a02] has quit [Changing host] | 02:28 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 02:28 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 02:30 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:d81b:714a:2377:43e2] has joined ##hplusroadmap | 02:31 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:d81b:714a:2377:43e2] has quit [Changing host] | 02:31 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 02:31 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 02:41 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:1819:9e89:4058:b940] has joined ##hplusroadmap | 02:43 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:1819:9e89:4058:b940] has quit [Changing host] | 02:43 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 02:43 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 02:45 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 02:46 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 02:46 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 02:46 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 02:55 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 03:32 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 03:39 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 03:39 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 03:39 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 03:39 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 03:43 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:1819:9e89:4058:b940] has joined ##hplusroadmap | 03:43 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:1819:9e89:4058:b940] has quit [Changing host] | 03:43 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 03:43 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-npshmknaqggdtyyz] has joined ##hplusroadmap | 03:54 | |
| -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap | 03:58 | |
| -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 268 seconds] | 04:11 | |
| -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Quit: wrldpc1] | 04:28 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 04:36 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:b6:8cac:645d:68be] has joined ##hplusroadmap | 04:37 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:b6:8cac:645d:68be] has quit [Changing host] | 04:37 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 04:37 | |
| -!- crCr62U0 [~crCr62U0@mm-222-136-84-93.dynamic.pppoe.mgts.by] has joined ##hplusroadmap | 04:55 | |
| -!- wrldpc1 [~ben@125.170.232.135] has joined ##hplusroadmap | 04:56 | |
| -!- wrldpc1 [~ben@125.170.232.135] has quit [Quit: wrldpc1] | 05:20 | |
| -!- crCr62U0 [~crCr62U0@mm-222-136-84-93.dynamic.pppoe.mgts.by] has quit [Quit: Leaving] | 05:28 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 268 seconds] | 06:10 | |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-pnotboycuimihjqc] has joined ##hplusroadmap | 06:12 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 06:27 | |
| -!- Urchin [~urchin@unaffiliated/urchin] has quit [Ping timeout: 260 seconds] | 06:39 | |
| -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap | 06:54 | |
| -!- saturn2 [~visitant@unaffiliated/clone-of-saturn/x-2509460] has quit [Ping timeout: 256 seconds] | 06:55 | |
| -!- saturn2 [~visitant@unaffiliated/clone-of-saturn/x-2509460] has joined ##hplusroadmap | 06:58 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 07:06 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Ping timeout: 260 seconds] | 07:20 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 265 seconds] | 07:24 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20060fb89021ea63f39.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap | 07:29 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 07:31 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 265 seconds] | 07:50 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has quit [Remote host closed the connection] | 08:16 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has joined ##hplusroadmap | 08:17 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has joined ##hplusroadmap | 08:28 | |
| -!- malez_ [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 08:32 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 08:37 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20060fb89021ea63f39.ipv6.abo.wanadoo.fr] has quit [Ping timeout: 250 seconds] | 08:41 | |
| -!- malez_ [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Ping timeout: 268 seconds] | 08:42 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 08:43 | |
| -!- m4l3z_ [~m4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 08:54 | |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving] | 09:00 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has quit [Remote host closed the connection] | 09:11 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has joined ##hplusroadmap | 09:12 | |
| -!- andytosh1 [~apoelstra@wpsoftware.net] has joined ##hplusroadmap | 09:24 | |
| -!- andytoshi [~apoelstra@wpsoftware.net] has quit [Ping timeout: 256 seconds] | 09:27 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has quit [Remote host closed the connection] | 09:33 | |
| -!- sandeepkr [~sandeepkr@111.235.65.5] has joined ##hplusroadmap | 09:34 | |
| kanzure | hmph | 09:56 |
|---|---|---|
| JayDugger | Reconsider yesterday's mandatory memoirs for money motion? | 10:00 |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has joined ##hplusroadmap | 10:14 | |
| kanzure | huh? | 10:21 |
| kanzure | just hanging out in san francisco | 10:21 |
| kanzure | this conference is serving free saki and natto. | 10:29 |
| kanzure | have to listen to the live translation, though | 10:29 |
| JayDugger | Saké you mean? | 10:44 |
| kanzure | i don't claim to know, what am i doing | 10:47 |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:69b3:dfbc:4d82:b5e0] has joined ##hplusroadmap | 10:50 | |
| -!- Regex__ [~Cara@2601:1c0:8501:d159:79a4:5f4a:58c2:62a5] has quit [Ping timeout: 250 seconds] | 10:53 | |
| -!- jtimon [~quassel@186.31.134.37.dynamic.jazztel.es] has quit [Ping timeout: 260 seconds] | 10:59 | |
| -!- vicarion [~net@dyn-118-138-198-83.its.monash.edu.au] has quit [Remote host closed the connection] | 11:23 | |
| nmz787_ | hmm, $15k OBO "lightly used" "demonstrated OEM specifications" http://www.ebay.com/itm/Seiko-SMI3200-FIB-system-/201511626500 | 11:36 |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has joined ##hplusroadmap | 11:36 | |
| chris_99 | neat, ive never seen a FIB machine before on ebay | 11:38 |
| nmz787_ | wtf, this looks like the same one in pleasanton that was being given away for free (which I posted online about) https://sudoroom.org/pipermail/sudo-discuss/2015-July/010412.html | 11:39 |
| nmz787_ | same company, beam services | 11:39 |
| nmz787_ | :/ | 11:39 |
| nmz787_ | welp, there's a $15k slap in the DIY community's face, I guess | 11:40 |
| kanzure | hrm? | 11:40 |
| chris_99 | muchos weird | 11:40 |
| chris_99 | what does single beam fib machine mean, can it still image too? | 11:43 |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:69b3:dfbc:4d82:b5e0] has quit [Read error: Connection reset by peer] | 12:09 | |
| -!- Regex_ [~Cara@2601:1c0:8501:d159:69b3:dfbc:4d82:b5e0] has joined ##hplusroadmap | 12:10 | |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-npshmknaqggdtyyz] has quit [Quit: Connection closed for inactivity] | 12:10 | |
| kanzure | "Electrorheology leads to healthier and tastier chocolate" http://www.pnas.org/content/113/27/7399.abstract | 12:11 |
| -!- Orpheon [~Orpheon@89.106.62.81.dynamic.wline.res.cust.swisscom.ch] has quit [Read error: Connection reset by peer] | 12:21 | |
| nmz787_ | chris_99: yeah, it is relative to a "dual beam" which also has a column with an electron gun for electron imaging, rather than imaging using secondary electrons that the FIB knocks off during the basically ballistic interaction | 12:23 |
| nmz787_ | you can still damage/vaporize surfaces with electron beams, it is just different and usually much slower to damage objects | 12:24 |
| chris_99 | ah interesting so you couldn't really use something like that without damaging the sample more than a SEM? | 12:32 |
| nmz787_ | basically | 12:51 |
| nmz787_ | but you plan around it, or get a dual-beam | 12:52 |
| -!- M4l3z [~M4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving] | 12:52 | |
| chris_99 | gotcha | 12:58 |
| -!- ufoinc [~hazirafel@bzq-109-67-17-9.red.bezeqint.net] has quit [Quit: Leaving] | 13:04 | |
| -!- vicarion [~net@dyn-118-138-198-83.its.monash.edu.au] has joined ##hplusroadmap | 13:10 | |
| -!- augur [~augur@2601:645:4100:15f2:68fd:c453:18e1:7dcf] has joined ##hplusroadmap | 13:13 | |
| kanzure | "Silicon proven Verilog library for IC and FPGA designers" https://github.com/parallella/oh | 13:13 |
| nmz787_ | hmm, bunch of GROMACS stuff that Intel has done for optimization | 13:14 |
| nmz787_ | MPI and Xeon stuff at least | 13:14 |
| nmz787_ | https://software.intel.com/en-us/node/536449 | 13:16 |
| nmz787_ | .title | 13:16 |
| yoleaux | Life Sciences | Intel® Software | 13:16 |
| nmz787_ | http://ipccsb.dfci.harvard.edu/ | 13:17 |
| nmz787_ | .title | 13:17 |
| yoleaux | Intel® PCCSB - The Intel® Parallel Computing Center for Structural Biology | 13:17 |
| nmz787_ | https://software.intel.com/en-us/articles/recipe-relion-for-intel-xeon-phi-7250-processor?language=ru | 13:18 |
| nmz787_ | .title | 13:18 |
| yoleaux | Recipe: RELION for Intel® Xeon Phi™ 7250 processor | Intel® Software | 13:18 |
| nmz787_ | "RELION is an image processing software package and widely used to achieve high resolution Cryo-EM structures. It uses Bayesian MAP+EM algorithm to provide more reliable structures than existing methods which is more suitable for heterogeneous data. RELION is distributed under a GPL license, it is completely free, open-source software for both academia and industry." | 13:18 |
| nmz787_ | http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page | 13:19 |
| nmz787_ | "RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior ... | 13:19 |
| nmz787_ | ... knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) ... | 13:19 |
| nmz787_ | ... JSB. If RELION is useful in your work, please cite at least one of these papers." | 13:19 |
| nmz787_ | from a single image of a fake membrane with some membrane-attached proteins, some guy showed he was able to extract all the various states of the protein configuration (ion channel), and then played a movie of the motions | 13:21 |
| nmz787_ | RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: ... | 13:22 |
| nmz787_ | ... the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB. If ... | 13:23 |
| nmz787_ | ... RELION is useful in your work, please cite at least one of these papers. | 13:23 |
| nmz787_ | whoops | 13:23 |
| nmz787_ | http://www.nature.com/articles/nature19948.epdf?author_access_token=SOCOH_Vpqzl_WtW9GCtuEtRgN0jAjWel9jnR3ZoTv0O0PJKHjrQ-upz3gxQ0K95mQMNLgeVM7OcHGXuXi_AiVIKx5cgzSF6L2Eyau_Tor--KSeaOez6Zk5h1NYRB7S10 | 13:23 |
| nmz787_ | "Unravelling biological macromolecules with cryo-electron microscopy" | 13:23 |
| nmz787_ | "When digital electron detectors became available commercially in 2013, the cryo-EM field was poised for a revolution. The unprec-edented image quality that arose from these detectors enabled orientations to be assigned with greater accuracy. The ability to separate particles from distinct structural states was also improved. Structures that heralded this era include the coenzyme F420-reduc-ing hydrogenase29, the mammalian ion channel ... | 13:25 |
| nmz787_ | ... transient receptor potential cation channel subfamilyVmember1 (TRPV1) (ref.30)" | 13:25 |
| nmz787_ | "the achievable limits of resolution now extend to below 3 Å (refs 2,35–37) and might even surpass 2 Å in favourable cases" | 13:26 |
| nmz787_ | "Beam-induced motion correction for sub-megadalton cryo-EM particles" | 13:28 |
| nmz787_ | the electron beam adds energy which increases diffusion :P | 13:29 |
| -!- augur [~augur@2601:645:4100:15f2:68fd:c453:18e1:7dcf] has quit [Read error: Connection reset by peer] | 13:31 | |
| -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap | 13:43 | |
| -!- augur [~augur@c-67-180-181-11.hsd1.ca.comcast.net] has joined ##hplusroadmap | 13:43 | |
| -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has joined ##hplusroadmap | 13:44 | |
| juri_ | nmz787_: what process resolution are you targeting? | 13:45 |
| -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 244 seconds] | 13:45 | |
| nmz787_ | juri_: hard to say, depends on what I am aiming to do | 13:46 |
| nmz787_ | some nanochannel gel-free separation stuff was done in 100nm wide channels I believe | 13:47 |
| juri_ | I'm asking, because i have some space CPU cycles, and was wondering what resolution to render your part at. | 13:47 |
| nmz787_ | that implicitcad test I think was in the microns | 13:47 |
| -!- wrldpc1 [~ben@p352135-ipngn200606kyoto.kyoto.ocn.ne.jp] has quit [Client Quit] | 13:47 | |
| nmz787_ | the field of posts, each post was like 5-10 microns I think in the original paper | 13:47 |
| juri_ | I've not only got ImplicitCAD rendering better, and faster, but i've built some nice boxes to do the rendering on. | 13:48 |
| juri_ | I'm ordering one with 280GFLOPS next. | 13:48 |
| nmz787_ | it was actually not something I personally wanted to use, rather I was at home on Christmas vacation and saw someone else (a more wet lab prone guy) mentioning that paper that described that part | 13:49 |
| nmz787_ | I only programmed the model in implicitcad for fun really, and to test the waters with something that I thought less programmer-type people might find interesting | 13:49 |
| juri_ | *nods*. i'm just using it as a test of new capabilities. | 13:49 |
| nmz787_ | yeah, cool | 13:49 |
| nmz787_ | I haven't had time to try running it myself here | 13:50 |
| juri_ | until about a month ago, ImplicitCAD had defects that made a rendering of something so small useless. | 13:50 |
| juri_ | it got 'blurry' at a micron or so. | 13:51 |
| nmz787_ | juri_: http://link.springer.com.sci-hub.cc/article/10.1007%2Fs10404-010-0720-2 | 13:52 |
| nmz787_ | "The square in the red dotted line corresponds to b. Double micro-column (30 um in length, 20 um in width, and 55 um in height) lines in each chamber were designed to promote trapping of protoplasts while the seeding process (b). The gap between micro-columns was 20 um" | 13:53 |
| nmz787_ | so 20 microns per post, with 20 microns between each post | 13:55 |
| nmz787_ | but I feel like I coded it to be parametric | 13:55 |
| nmz787_ | yeah, the post diameter is this https://github.com/nmz787/microfluidic-cad/blob/master/implicitCAD/tobacco_mesophyll_protoplast_fusion_device.escad#L480 | 13:56 |
| nmz787_ | pitch https://github.com/nmz787/microfluidic-cad/blob/master/implicitCAD/tobacco_mesophyll_protoplast_fusion_device.escad#L483 | 13:56 |
| nmz787_ | so those whole-numbers are assumed to be microns | 13:56 |
| juri_ | oh, so i can just render this an 1, and tell you to scale it down. ;) | 14:01 |
| juri_ | that's no fun. ;) | 14:05 |
| kanzure | we need a light-emitting protein that sticks to cytoskeletons | 14:17 |
| nmz787_ | juri_: well it wasn't yielding good results however it was that I was running it, that's all I recall | 14:22 |
| nmz787_ | at the top of the escad file I see it says quality=400... I seem to recall some things being nicely rounded, and others ghastly off and blocky, and I think there was a large plane that should have been a few large triangles, but ended up being many many more than needed and bloated the output significantly | 14:25 |
| nmz787_ | (STL) | 14:25 |
| nmz787_ | juri_: so I guess I would ideally hope for resolution down to at least 0.1 microns, that seems like a reasonable number (that might be my cheap $100 laser etcher step size) | 14:27 |
| nmz787_ | oh, maybe it is higher than that | 14:27 |
| nmz787_ | it would be a good number for DPL lithography | 14:28 |
| nmz787_ | err, DLP | 14:28 |
| juri_ | grinding down to 0.1 now. | 14:33 |
| -!- justanotheruser [~justanoth@unaffiliated/justanotheruser] has joined ##hplusroadmap | 14:33 | |
| -!- justan0theruser [~justanoth@unaffiliated/justanotheruser] has quit [Ping timeout: 252 seconds] | 14:36 | |
| kanzure | "Decentralized anonymous micropayments" https://eprint.iacr.org/2016/1033.pdf | 14:38 |
| archels_ | http://www.theverge.com/circuitbreaker/2016/11/4/13526198/zotac-vr-go-backpack-computer | 14:41 |
| -!- andytosh1 is now known as andytoshi | 14:48 | |
| -!- JenElizabeth [~Jen@cpc76808-brmb10-2-0-cust35.1-3.cable.virginm.net] has quit [Ping timeout: 250 seconds] | 14:50 | |
| kanzure | http://theconversation.com/atlantropa-the-colossal-1920s-plan-to-dam-the-mediterranean-and-create-a-supercontinent-47370 | 14:54 |
| -!- augur [~augur@c-67-180-181-11.hsd1.ca.comcast.net] has quit [Remote host closed the connection] | 15:19 | |
| -!- augur [~augur@2601:645:4100:15f2:7880:5bf7:aa5b:f807] has joined ##hplusroadmap | 15:20 | |
| kanzure | need protein complex that attaches arbitrary dna barcodes to arbitrary dna messages | 15:23 |
| nmz787_ | ligase? | 15:24 |
| -!- augur [~augur@2601:645:4100:15f2:7880:5bf7:aa5b:f807] has quit [Ping timeout: 260 seconds] | 15:25 | |
| -!- augur [~augur@2601:645:4100:15f2:7d51:bca9:b22:6436] has joined ##hplusroadmap | 15:25 | |
| -!- CheckDavid [uid14990@gateway/web/irccloud.com/x-pnotboycuimihjqc] has quit [Quit: Connection closed for inactivity] | 15:33 | |
| kanzure | nmz787_: well, it needs to be a specific ligase i think | 16:01 |
| kanzure | nmz787_: because you only want the dna barcode to get attached in a highly preferential manner | 16:01 |
| kanzure | at some very high binding affinities | 16:01 |
| -!- m4l3z_ [~m4l3z@LFbn-1-4220-37.w92-169.abo.wanadoo.fr] has quit [Quit: Leaving] | 16:15 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20028fa029eb8b4aa9d.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap | 16:16 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20028fa029eb8b4aa9d.ipv6.abo.wanadoo.fr] has quit [Quit: Leaving] | 16:45 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20028fa029eb8b4aa9d.ipv6.abo.wanadoo.fr] has joined ##hplusroadmap | 16:46 | |
| -!- chris_99 [~chris_99@unaffiliated/chris-99/x-3062929] has quit [Quit: Leaving] | 16:49 | |
| -!- m4l3z [~m4l3z@2a01cb040226f20028fa029eb8b4aa9d.ipv6.abo.wanadoo.fr] has quit [Quit: Leaving] | 16:51 | |
| -!- nildicit__ [~nildicit@unaffiliated/nildicit] has joined ##hplusroadmap | 16:58 | |
| -!- nildicit_ [~nildicit@unaffiliated/nildicit] has joined ##hplusroadmap | 17:00 | |
| -!- nildicit__ [~nildicit@unaffiliated/nildicit] has quit [Ping timeout: 265 seconds] | 17:02 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 18:49 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 18:49 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 18:49 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 19:25 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:c56c:6885:2834:7569] has joined ##hplusroadmap | 19:25 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:c56c:6885:2834:7569] has quit [Changing host] | 19:25 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 19:25 | |
| -!- esmerelda [~andares@unaffiliated/jacco] has quit [Ping timeout: 260 seconds] | 20:15 | |
| wrldpcmbl | Wish I could vote for AI. | 20:15 |
| -!- PatrickRobotham [uid18270@gateway/web/irccloud.com/x-jsolkaozqyvaqved] has joined ##hplusroadmap | 20:21 | |
| -!- Dumuzi [Dumuzi@Dont.Blink.PanicBNC.org] has quit [Ping timeout: 245 seconds] | 20:30 | |
| -!- Dumuzi [Dumuzi@Dont.Blink.PanicBNC.org] has joined ##hplusroadmap | 20:34 | |
| -!- JenElizabeth [~Jen@cpc76808-brmb10-2-0-cust35.1-3.cable.virginm.net] has joined ##hplusroadmap | 20:40 | |
| -!- nildicit_ [~nildicit@unaffiliated/nildicit] has left ##hplusroadmap ["Leaving"] | 20:46 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 265 seconds] | 21:17 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 21:28 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 21:31 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 21:31 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 21:31 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 22:00 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:cc09:abdb:d7ee:642a] has joined ##hplusroadmap | 22:02 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:cc09:abdb:d7ee:642a] has quit [Changing host] | 22:02 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 22:02 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 22:09 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has joined ##hplusroadmap | 22:09 | |
| -!- ebowden [~ebowden@CPE-101-180-233-178.lnse3.cha.bigpond.net.au] has quit [Changing host] | 22:09 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 22:09 | |
| -!- yashgaroth [~yashgarot@2602:306:35fa:d500:f5e0:f867:a11d:8d52] has quit [Quit: Leaving] | 22:36 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Remote host closed the connection] | 22:55 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has joined ##hplusroadmap | 22:56 | |
| -!- Orpheon [~Orpheon@213.200.193.129] has joined ##hplusroadmap | 22:58 | |
| kanzure | hrm | 23:09 |
| superkuh | Then you get something like this short science fiction story, http://superkuh.com/TSB-Resistance.txt (Resistance, by Tobias S. Buckell) | 23:12 |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:cc09:abdb:d7ee:642a] has joined ##hplusroadmap | 23:15 | |
| -!- ebowden [~ebowden@2001:8003:103c:ee00:cc09:abdb:d7ee:642a] has quit [Changing host] | 23:15 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 23:15 | |
| -!- ebowden_ [~ebowden@2001:8003:103c:ee00:f4e3:6521:ff08:12ab] has joined ##hplusroadmap | 23:17 | |
| -!- ebowden_ [~ebowden@2001:8003:103c:ee00:f4e3:6521:ff08:12ab] has quit [Changing host] | 23:17 | |
| -!- ebowden_ [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap | 23:17 | |
| -!- ebowden [~ebowden@unaffiliated/ebowden] has quit [Ping timeout: 240 seconds] | 23:20 | |
| -!- Gurkenglas [~Gurkengla@dslb-178-005-217-051.178.005.pools.vodafone-ip.de] has quit [Ping timeout: 268 seconds] | 23:48 | |
| -!- esmerelda [~andares@unaffiliated/jacco] has joined ##hplusroadmap | 23:55 | |
| --- Log closed Sun Nov 06 00:00:42 2016 | ||
Generated by irclog2html.py 2.15.0.dev0 by Marius Gedminas - find it at mg.pov.lt!