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| kanzure | yep.. | 03:25 |
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| JayDugger | Ah-huh. | 05:45 |
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| kanzure | .title https://news.ycombinator.com/item?id=12973223 | 06:43 |
| yoleaux | SpaceX FCC Application for over 4000 Internet Satellites | Hacker News | 06:43 |
| kanzure | .title https://news.ycombinator.com/item?id=12976393 | 06:46 |
| yoleaux | The mathematics of science's broken reward system | Hacker News | 06:46 |
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| gnusha | https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=29dd6622 Bryan Bishop: link to adlai/joinmarket slides >> http://diyhpl.us/diyhpluswiki/transcripts/scalingbitcoin/milan/joinmarket/ | 08:09 |
| kanzure | "In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration" http://www.nature.com/nature/journal/vaop/ncurrent/full/nature20565.html | 08:11 |
| kanzure | "Targeted genome editing via engineered nucleases is an exciting area of biomedical research and holds potential for clinical applications. Despite rapid advances in the field, in vivo targeted transgene integration is still infeasible because current tools are inefficient1, especially for non-dividing cells, which compose most adult tissues. This poses a barrier for uncovering fundamental biologica | 08:11 |
| kanzure | l principles and developing treatments ... | 08:11 |
| kanzure | ... for a broad range of genetic disorders2. Based on clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9)3, 4 technology, here we devise a homology-independent targeted integration (HITI) strategy, which allows for robust DNA knock-in in both dividing and non-dividing cells in vitro and, more importantly, in vivo (for example, in neurons of postnatal mammals). As a proof of c | 08:11 |
| kanzure | oncept of its therapeutic potential, we ... | 08:11 |
| kanzure | ... demonstrate the efficacy of HITI in improving visual function using a rat model of the retinal degeneration condition retinitis pigmentosa. The HITI method presented here establishes new avenues for basic research and targeted gene therapies." | 08:11 |
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| kanzure | weird that there was a linebreak in "fundamental biological principles" | 08:12 |
| kanzure | something about a mutation of "Sik3 protein kinase" and a "missense, gain-of-function mutation in the sodium leak channel NALCN" as impacting total sleep duration and REM sleep duration http://www.nature.com/nature/journal/v539/n7629/full/nature20142.html | 08:17 |
| kanzure | "A missense, gain-of-function mutation in the sodium leak channel NALCN reduces the total amount and episode duration of REMS, apparently by increasing the excitability of REMS-inhibiting neurons" | 08:17 |
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| kanzure | "Tracking the ultrafast motion of a single molecule by femtosecond orbital imaging" http://www.nature.com/nature/journal/v539/n7628/full/nature19816.html | 09:11 |
| kanzure | elsewhere: "... Genetic exclusion of CB1 receptors from hippocampal mitochondria prevents cannabinoid-induced reduction of mitochondrial mobility, synaptic transmission and memory formation. mtCB1 receptors signal through intra-mitochondrial G?i protein activation and consequent inhibition of soluble-adenylyl cyclase (sAC). The resulting inhibition of protein kinase A (PKA)-dependent phosphorylatio | 09:13 |
| kanzure | n of specific subunits of the ... | 09:13 |
| kanzure | ... mitochondrial electron transport system eventually leads to decreased cellular respiration. Hippocampal inhibition of sAC activity or manipulation of intra-mitochondrial PKA signalling or phosphorylation of the Complex I subunit NDUFS2 inhibit bioenergetic and amnesic effects of cannabinoids. Thus, the G protein-coupled mtCB1 receptors regulate memory processes via modulation of mitochondrial en | 09:13 |
| kanzure | ergy metabolism" | 09:13 |
| kanzure | hmm i think this is because of a recent irssi upgrade | 09:13 |
| __mz_o | what the message splitting? | 09:22 |
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| nmz787_w | hmm, well that GME (genetically modified edit??? come join my new acronym bandwagon or give me a better jargon!) is definitely something that sounds like I want to stay away from | 09:41 |
| nmz787_w | I guess good for reverse engineering us | 09:41 |
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| kanzure | hrmph | 20:00 |
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| --- Log closed Fri Nov 18 00:00:28 2016 | ||
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