- A 15-liter DIY bioreactor design for fed-batch fermentations was shared, along with a link to its construction paper (Gueguim-Kana et al., African Journal of Biotechnology, 2003).  
- Discussion centered on building an open-source DNA synthesizer: the Pogo BioCompiler project was cited, with its published $23 k BOM being criticized as inflated; participants argued the motion-control and electronics could be reduced to $500–$1 k.  
- A vision for an ultra-low-cost biohacking lab was outlined, including a human-powered centrifuge made from a broken bicycle and scavenged electric motors.  
- The group debated the culture of self-experimentation: PIHKAL/TIHKAL (Shulgin’s phenethylamine/tryptamine memoirs) were referenced, with criticism that recreational psychonauts lack rigorous, brain-centric research protocols.  
- NIST’s new “Open Ontology Repository” (OOR) for building reasoning “thinking machines” was announced; participants saw it as a potential knowledge base for AI-driven bio-design.  
- Frustration was voiced over the dated, corporate-style transhumanism.org website; the new webmaster contemplated a technical overhaul to better serve DIY-bio, AI, and neuro-enhancement communities.  
- Bootstrapping strategies were proposed: using a bioreactor to make lab reagents, coupled with an automated manufacturing pipeline (fablab/SKDB) to replicate more lab hardware.