- A low-cost open-source DNA oligo-synthesizer called POSAM (Piezoelectric Oligonucleotide Synthesizer Array Microfluidics) was described; it uses inkjet heads to make ~25-mer oligos that are then ligated with T4 ligase, relies on PTFE/glass/silicone microfluidics, and needs high-purity inert gas for solvent handling.

- Participants debated the economics of ultracentrifuges versus open-source algae bioreactors, concluding that benchtop equipment and Narsi’s DIY bioreactor project are more practical than a $100 k ultracentrifuge for most biohackers.

- SBML (Systems Biology Markup Language) was proposed as the biological equivalent of SolidWorks SLDPRT files, and kanzure’s curated repositories (http://heybryan.org/gitweb.cgi and the Ellingtonia biotech archive) were offered as open-source parts libraries for synthetic-biology CAD.

- A detailed 3- to 4-year “Transhuman Tech” university curriculum was sketched, covering cybernetics, genetic engineering, neurology, HMI, media appreciation (e.g., Ghost in the Shell), prospective studies, innovation management, mathematical modeling, law, and rapid-learning techniques, with Wikiversity floated as the organizing platform.

- Monsanto’s terminator-seed business model was dissected as a cautionary tale of IP lock-in and dependency creation, sparking debate on the ethics of working for such firms and how to vet funding sources for open biotech projects.

- kanzure is seeking contact with Prof. Kaznessis (U. Minnesota) about synthetic biopharmaceuticals and antimicrobial-peptide design, and is concerned that the DIY-bioreactor project may still require ultra-fine filtration even with bio-flocculant coagulation.

- A large public collection of scientific books (Feynman lectures, chemistry, biology) is hosted at http://heybryan.org/books/, and participants briefly considered but dismissed torrent distribution for the archive.