- A discussion on DNA polymerase subunit structure revealed that Saccharomyces cerevisiae Pol ε comprises four subunits (Pol2, Dpb2, Dpb3, Dpb4) and that kanzure hosts ~20 PDFs on polymerase at http://heybryan.org/~bbishop/docs/ellingtonia/polymerase/.

- Drazak proposed a sequencing-by-synthesis scheme that detects pyrophosphate (PPi) release via a fluorescent or luminescent dye, asking whether the free-energy change differs among dATP, dCTP, dGTP and dTTP incorporation.

- Kanzure circulated a two-page draft titled “Towards automatic generation of linear genetic regulatory networks via BioBricks” intended for BMC Systems Biology; nsh offered to review despite never having published.

- A debate on AGI safety erupted after an AGI mailing-list post claimed to outline a plan to “neutralize AGI and other dangerous technologies”; participants mocked the idea as akin to “neutralizing bacteria” and criticized Eliezer Yudkowsky’s “Friendly AI first” strategy.

- Gene explored building a sub-$20, 4 µm-resolution electrochemical micromachining mill from LEGO and CD-player linear actuators; fenn countered that sub-micron CNC accuracy requires preloaded ballscrews or glass scales and that CD mechanisms are unsuitable.

- Kanzure prototyped a Python/OpenGL “gears.py” script that visualizes GraphSynth-generated graphs as 3-D gears, later pivoting toward VTK and PyVTK for CAD functionality after surveying pythoncad and NeHe lesson code.

- Links to open-manufacturing resources were shared, including Thingiverse (noted for lax file-type upload), the reconfigurable Unimat desktop tool, and dumpsterCNC for DIY linear-motion parts.

- The channel noted the Rosetta macromolecular-modeling blog, the bioknoppix live CD for molecular biologists, and an upcoming biofuel/algae conference that kanzure planned to attend.

- Origami automation was a recurring theme: kanzure examined SCORE/WebEOS APIs for programmatic folding, while fenn referenced Theo Jansen’s genetic-algorithm-optimized “Strandbeest” mechanism and its papercraft incarnation.

- Miscellaneous links included a Google-video “Star Trek: New Voyages” parody, a geocaching “find the money” game, and a Nature news piece on “genomic dark matter” that prompted nsh’s exasperated “ARGHGHGHGHGH”.