• A 19-year-old undergraduate proposed using viral vectors to insert genes from the “immortal jellyfish” Turritopsis dohrnii into humans to trigger controlled reversion of somatic tissues to a younger state, but the channel concluded the mechanism is uncharacterized and cnidarian-mammalian pathways are too divergent for a simple gene swap.

• Kanzure provided a curated reading package: a 2009 gene-therapy vector review (http://designfiles.org/papers/gene-therapy/), the 1996 Piraino et al. Biological Bulletin paper on Turritopsis life-cycle reversal, and a 2008 review on Turritopsis dohrnii aging (http://www.sciencepub.net/nature/ns0802/03_1279_hongbao_turritopsis_ns0802_15_20.pdf).

• Whole-genome sequencing costs were mapped from ~$1 M (2000) to ~$10 k (2010) and projected below $100 within a year; options discussed include university services, eBay used sequencers ($300–$25 k), or DIY hardware builds.

• The channel debated whether jellyfish-based rejuvenation is more practical than stem-cell or iPSC approaches, concluding that extensive comparative genomics, SNP analysis, and molecular groundwork are required before any translational work.

• CAD-to-3-D-printing workflow was clarified: solid-geometry CAD is “source,” STL is “binary”; PythonOCC was recommended for scripted CAD (model.to_stl()), and HeeksCAD (http://heekscad.org/) was offered as a GUI alternative.

• Synthetic-biology CAD tools were critiqued: ClothoCAD v1.0 (2008) was deemed stagnant and “not really CAD,” and a landscape diagram of SynBio tools was shared (http://clothocad.sourceforge.net/wiki/images/4/42/SynBioToolLandscape.png).

• An “8-bit Tools of Science” post (http://bookhling.wordpress.com/2010/07/03/8bit-tools-of-science/) showcased ultra-low-cost lab instrumentation, while a 34-page “Synthetic Biology Primer” PDF (http://openwetware.org/images/3/3d/SB_Primer_100707.pdf) was circulated as foundational reading, though only Part 1 appears to exist.

• Aubrey de Grey’s role was clarified as literature synthesis and theoretical work (e.g., WILT anti-cancer strategy) rather than bench research; his collected papers were archived at http://heybryan.org/transhuman/Aubrey.zip.

• R was praised for automating data analysis; an anecdote described using R scripts to analyze UV/Vis spectra of I₂ vapor, surpassing 1970s handbook accuracy.

• No canonical list of active channel projects exists, but pointers were given to an old wiki (http://www.acceleratingfuture.com/wiki/Hplusroadmap_wiki) and a master TODO in YAML (http://designfiles.org/skdb/doc/proposals/trans-tech.yaml), with an open invitation to crowd-source an updated inventory.