• A proposal for an EEG-controlled 3-D printer that would let users “think” objects into existence was floated, but participants concluded that current EEG resolution and printer fidelity are too low and too expensive for practical use.

• Open-source, low-cost DNA synthesis hardware was discussed as a strategic step toward on-demand genetic engineering; current commercial prices (~$0.35 / bp for custom oligos, ~$0.01 / bp on microarrays) were cited, and two key papers on high-fidelity gene synthesis and microchip-based scalable synthesis were archived at diyhpl.us.

• Atomic-force-microscopy “nanoneedles” were highlighted for single-cell transfection: recent work shows 30 % plasmid-delivery efficiency, recovery/amplification of single plasmids on the AFM tip, and high-efficiency DNA injection into human mesenchymal stem cells—participants dubbed the method “DNA stabbing.”

• A debate over what belongs on an “h+ roadmap” pitted those who view DNA synthesis as too distant from human enhancement against others who cited near-term gene-therapy examples (curing color-blindness, adding infrared opsins) as concrete transhumanist wins.

• Computational neuron-modeling needs were explored: participants sought an automated, scriptable pipeline to generate smooth, tapered mesh extrusions from ball-and-stick neuron data, with recommendations to investigate CGAL, OpenSCAD, OpenCASCADE, and Blender; Markram et al.’s 2012 “neuron membrane mesh representation” paper was shared as a state-of-the-art reference.

• Historical microfluidic DNA-synthesis platforms were reviewed: Motorola Life Sciences’ CodeLink microarray (1998–2002), Xeotron’s 2003 “PicoArray” oligo synthesizer (10 kb assemblies, $45 M sales before Invitrogen acquisition), and the opaque pricing of 454/Roche picotiter plates (~29 µm wells, 1.7 M parallel pyrosequencing reactions).

• Novel bench-top transfection methods were proposed: LED-assisted photoporation (405 nm violet diode) for cells on nanoscale chips, and optical-tweezer-directed “one-bead one-sequence” DNA synthesis in microfluidic channels.

• Broader speculative concepts included an immune-system “upgrade” using intracellular protein sentinels to recognize and destroy viral DNA/RNA signatures, and directed viral-evolution competitions to stay ahead of pathogen mutation.

• The LH001 open-source biohacking device was criticized for its painful user interface; participants urged creation of a concise demo video and clearer documentation to improve adoption and collaboration.