- Deisseroth’s lab has developed a high-throughput method for whole-brain, cellular-resolution imaging of fixed mouse brains without sectioning or reconstruction, using antibody labeling and computational 3-D modeling; the technique is described in Mikula et al., Nature Methods (21 Oct 2012).  
- The NanoEngineer-1 molecular CAD platform is back online, including a wiki page detailing a proposed “Sorting Pump” nanomechanical device for molecular manipulation.  
- A new DIYbio community survey was circulated at surveys.peerproduction.net/ls/index.php?sid=88161&lang=en to collect data on grassroots biology practices.  
- Paul Graham’s essay “Hardware” (paulgraham.com/hw.html) was shared as a reference on the challenges and opportunities of hardware startups.  
- The basementbiotech.org site was highlighted as a resource for low-cost, open-source biotechnology projects.  
- Channel members discussed the practicality and availability of DIY trans-cranial direct-current stimulation (tDCS) caps, with one participant asking for working designs.  
- Ray Kurzweil appeared—apparently unbilled—in an in-flight Jaguar/Lincoln commercial, prompting surprise and disappointment among viewers about his commercial endorsements.