- A DIY micro-stereolithography printer is being designed to expose photopolymer layers as thin as 400 nm with either a DLP projector or a scanned laser, aiming to create high-resolution PDMS masters for microfluidic devices; candidate resins include Sigma-Aldrich polyacrylamide (~$150 kg⁻¹) and Bucktown Polymers UV/visible-curable resins ($235–$1040 gal⁻¹).  
- yashgaroth outlined a non-viral gene-therapy protocol for personal muscle enhancement: a plasmid over-expressing human follistatin (myostatin inhibitor) delivered by in-vivo electroporation, with plasmid DNA purified to <1 EU/mg endotoxin and verified by LAL assay; the same workflow could in principle express HGH, but sourcing the gene is legally sensitive.  
- The channel revived interest in open-source DNA synthesis hardware, specifically a laser-etched “PicoArray” microfluidic synthesizer (Xeotron 2003) capable of 10 kb assemblies from 10⁶ parallel oligo spots; a <$300 DLP projector or hacked laser cutter could print micron-scale photopolymer molds for PDMS chips, cutting reagent costs to ~2–4 ¢ per prototype layer.  
- George Church’s 2009 projection of $1.5 k whole-genome sequencing (40×) was contrasted with today’s $4 k bulk pricing, while his list of 20+ biotech co-foundings (including Cambrian Genomics 2011-2012) was mined for clues about abandoned advisory roles and the economics of locked-down commercial platforms.  
- Gut-bacteria engineering was proposed as a safer DIY target—engineering commensals to synthesize vitamins or essential amino acids—while the San Diego DIYbio lab secured city approval for a $1/year lease plus utilities, providing a legal facility for plasmid prep, tissue-culture hood, and endotoxin testing.  
- kanzure released “python-requestions,” a library that caches real HTTP responses for deterministic unit testing of code that relies on the python-requests library.