- TALENs (Transcription Activator-Like Effectors) were highlighted as a powerful and frequently discussed genome-engineering tool; the open resource taleffectors.genome-engineering.org was shared for reagents, protocols, and design tips.  
- Optogenetics.org was introduced as a centralized resource for light-controlled neural modulation techniques and associated hardware/software.  
- The feasibility of creating a “blank” or chassis cell that accepts arbitrary DNA was debated; naturally competent organisms such as Helicobacter pylori and Acinetobacter sp. BD413 were cited, along with links to relevant papers on competence proteins and transformation protocols.  
- A practical question was raised about cloning proprietary plants from dried leaf material, prompting discussion of DNA extraction, codon optimization, and the limits of current plant transformation methods.  
- The diyhpl.us wiki was updated with new entries for community biology labs: HiveBio (Seattle) and BiotechnBeyond (Carlsbad), and Josh Perfetto added himself to the Sunnyvale group list.  
- Gradstudentbot, a humorous IRC bot that outputs stereotypical graduate-student phrases, was showcased as an example of tolerated automation in contrast to the general aversion to bots in the channel.  
- Links to TED talks and anime with biotech themes—Jonathan Trent’s floating algae pods for energy, Moyashimon: Tales of Agriculture, and Element Hunters—were circulated as cultural references relevant to DIY biology and transhumanist interests.