• A PNAS paper on “Bioengineered functional brain-like cortical tissue” was shared, documenting the successful growth of 3-D cortical tissue that exhibits native-like electrical activity and layered architecture, marking a step toward lab-grown brain models for research and repair.

• Neurokernel, an open-source Python framework, was highlighted for its ability to emulate the entire Drosophila brain across multiple NVIDIA GPUs by exploiting the fly’s highly modular neuronal structure to minimize inter-module communication overhead.

• A Nature Biotechnology paper on “spatially localized subsampling of the transcriptome” was discussed, describing a method for performing single-cell mRNA sequencing on individual neurons within an in-vitro brain slice, enabling precise molecular profiling of specific cells.

• Participants debated the practicality of in-vivo DNA/RNA sequencing, concluding that whole-genome sequencing from a single cell’s DNA remains infeasible, whereas targeted cDNA amplification or patch-clamp-guided mRNA harvesting offers more realistic routes to in-situ transcriptomics.

• The channel circulated links to reusable open-source tools: verbNURBS, a JavaScript NURBS geometry library with unit tests, and the Neurokernel GitHub repository, illustrating the community’s emphasis on freely available software for neuroscience and bioengineering.

• A Bitcoin technical post on exploiting the SIGHASH_SINGLE signature type and a 1.2 GB Cryptome torrent archive (June 2014) were shared, reflecting ongoing interest in cryptographic security and large-scale freedom-of-information datasets within the transhumanist community.

• A page from the manga “Akumetsu” (chapter 91, volume 11) was linked, suggesting discussion of its themes of vigilante biotech justice and anti-corruption transhumanist tactics as cultural commentary relevant to the channel’s interests.