- A micro-fluidic DNA-sequencing concept was proposed that replaces expensive reversible terminators with a thermo-regulated terminal deoxynucleotidyl transferase (TdT) and millisecond laser or resistive heating pulses to add exactly one nucleotide at a time, eliminating chemical terminators entirely.  
- Participants debated the engineering risk of combining multiple low-Technology-Readiness-Level (TRL) components in a single device, concluding that success probability drops sharply as complexity rises; they advocated maximizing mature techniques such as cyclic voltammetry for single-nucleotide detection.  
- An open-source mask-less photolithography platform was described, built from repurposed Blu-ray optics, MIC-6 aluminum plates, and low-cost CNC rails, achieving sub-micron XY positioning with 28BYJ-48 or NEMA steppers driven by Arduino sine-wave look-up tables and Allegro driver chips.  
- A cell-free protein-synthesis scheme was outlined in which a single, reusable mRNA template is combined with a minimal 2 × 21 tRNA library (AAA and GGG codons) to iteratively assemble very long polypeptides like titin (~33 000 aa) without cloning large genes, though ribosome pausing, wash stability, and enzyme cost remain open questions.  
- Directed evolution was suggested as a route to obtain ribosomes or polymerases tolerant of long pauses or harsh chemistries, but participants noted the difficulty of designing selection schemes for multi-step synthesis tasks.  
- Recent literature on electrically conductive Geobacter pili, voltage-sensing Ci-VSP phosphatases, and nanoporous-gold-encapsulated cytochrome-c biosensors was highlighted as promising building blocks for bio-electronic interfaces.  
- Practical DIYbio constraints were catalogued: phosphoramidite reagents degrade quickly once dissolved, microfluidic chips suffer moisture and oxygen ingress, and bulk purchasing often exceeds reagent shelf life.  
- Key resources shared include the 2012 Nucleic Acids Research paper on “Lightning Terminators,” LaserGen’s web page, Princeton’s 2008 nanochannel voltammetry thesis, the Global Alliance for Genomics & Health search engine, TinyRAM (a minimal RISC ISA for verifiable computation), open-source GRBL/Arduino CNC controllers, and the Bus Pirate debugging tool.