- A pre-print titled “Wireheading has many positive effects and few negative ones” (http://www.turingbrains.com/temp/wireheading.pdf) argues that risk-free electrode-based removal of negative emotions is beneficial, sparking debate on whether such emotional editing would impair cognition.

- NanoEngineer-1, an open-source molecular-design package rescued at https://github.com/kanzure/nanoengineer, is considered a useful starting point for Drexlerian nanotech design but is crippled by an inaccurate classical MD engine (MM2/NanoDynamics) that yields non-physical results.

- Transcriptic’s cloud-lab architecture relies on a NASA-derived constraint solver to schedule robotic wet-lab equipment for maximal freezer, pipette, and incubator utilization; most early hardware came from a $50 k liquidation of a defunct biolab rather than custom engineering.

- A 2014 peanut-allergy trial (J Allergy Clin Immunol) reported 82 % sustained unresponsiveness when oral immunotherapy was combined with Lactobacillus rhamnosus probiotic, raising questions about immune-suppression risks and the need for gut-colonization assays.

- Capital constraints on transhumanist experimentation were highlighted: $20 k thermocyclers, $500 k CNC mills, $2 M AFMs, and $250 k–$3 M fast-food franchises inflate hardware costs and keep DIY biology, nanotech, and fabrication out of reach; zero-interest, low-risk loans were proposed as a systemic fix.

- A critical-path heuristic was added to the DIYH+ wiki: “a sequence of tasks such that if any task takes one minute longer, the whole project takes one minute longer—tasks on the critical path have zero slack.”

- Current molecular-dynamics simulators (GROMACS, NanoEngineer) either don’t scale or produce non-physical results for Drexler-style nanomachinery; a performant, physically accurate MD engine is still missing.

- An AGI reading list was assembled: start with Russell & Norvig’s AIMA, then proceed to AGI conference proceedings and Goertzel’s “Engineering General Intelligence” for an OpenCog-biased but broad overview.

- A cryonics breeding program was proposed: selecting pets (and eventually humans) for freeze-thaw survivability over generations, using the resulting genetic insights to enable human cryopreservation.

- A 2009 fluorescence-spectroscopy review (http://theinstituteoffluorescence.com/PDF/10%20Chris%20Pub.pdf) documents how picosecond dye and femtosecond Ti:sapphire lasers, plus fluorescent DNA probes replacing radioactive labels during the Human Genome Project, enabled time-resolved fluorescence decay data to yield richer molecular information than earlier static measurements.