• A new whole-brain connectome protocol, BROPA (brain-wide reduced-osmium staining with pyrogallol-mediated amplification), published in Nature Methods, enables serial block-face EM of an entire mouse brain at synapse-level resolution, making full neuronal tracing feasible.  

• Rational-design antibiotic cycling strategies, detailed in PLOS ONE, use evolutionary models (CPM/EPM) to compute treatment sequences that revert TEM-β-lactamase mutants to drug-susceptible wild-type, offering a concrete method to reverse antibiotic resistance.  

• Participants debated whether high-resolution EM datasets—such as 24 MB images of C. elegans and mouse brains—contain sufficient structural information to reconstruct functional neural “scripts,” sidestepping philosophical questions of consciousness or identity.  

• Galantamine, an acetylcholinesterase inhibitor from daffodil bulbs, was proposed as a nicotine-like nootropic with fewer side-effects; the red spider lily (Lycoris radiata) was noted as an alternative botanical source with cultural symbolism.  

• A discussion on transhumanist feasibility questioned non-governmental space access and stellar-scale thermodynamic limits, countered by examples of private launch ventures and the argument that post-human intelligence could solve such constraints.  

• The channel’s automated paper-fetching bot, paperbot, was down with a “Connection refused” error; its source code is available at https://github.com/kanzure/paperbot for debugging and redeployment.  

• Participants argued that wet-lab biologists rarely program because current tools are “too finicky,” creating a cultural barrier; cross-disciplinary technologists who automate biology or chemistry workflows gain outsized leverage compared to pure CS talent.