• The channel dissected Henry Markram’s credibility, circulating a 2006 transcript to ground critiques of the “reality-distortion field” around Blue Brain and the Human Brain Project.  
• A practical open-source DNA-synthesis route was mapped out: repurpose Epson 6-channel inkjet heads (1.5 pL drops, 180 individually addressable nozzles per channel) by driving them with 4–30 V trapezoidal waveforms and shift-register latches, then scale by tiling heads for >100 parallel chemistries.  
• Technical risks of inkjet synthesis—drop-to-drop accuracy, diffusion-limited mixing, and micron-level misalignment between synthesis steps—were weighed against acceptable tolerances and LinuxCNC-based real-time synchronization using Mesa FPGA cards and optical encoders.  
• Nanopore-based single-molecule DNA synthesis was explored as an alternative, combining electrophoretic nucleotide delivery, femtoamp current sensing, and FIB-fabricated nanochannels to bypass traditional phosphoramidite chemistry.  
• A novel “size-up” polymerase engineering concept was proposed: iteratively enlarge DNA-like backbones (HNA, TNA, PNA, etc.) to 100 nm-scale nucleotide objects that can be handled by mutated polymerases and manipulated with macro-scale machines, then enzymatically reverted to natural DNA.  
• Participants highlighted a PNAS paper quantifying biological aging in young adults, arguing the field over-focuses on elderly cohorts and chronic disease while neglecting early-life biomarkers.  
• Commercial print-head survey: Epson (cheap, 100 nozzles, 5–9 kHz), Xaar 128/126 ($300, 126 nozzles, 35 pL), and Kyocera industrial heads (5,120 nozzles, 330 M drops/s, $8 k, NDA-locked) were compared, noting high-end heads now use LVDS/HDMI links and on-board firmware.  
• Shared resources included Mousera (“Heroku-for-mouse-studies”), Synaptic’s idle-university-equipment rental platform, pingfs (filesystem-over-ICMP), Sandia’s digital-microfluidic hub video, and the stalled Kilobaser benchtop DNA synthesizer project.