- The channel dissected DIY LED implants popularized by Grindhouse Wetware, criticizing the devices for large size, battery constraints, and failure to adopt wireless power transmission despite earlier advice.  
- Gene-therapy delivery was identified as the dominant bottleneck: while DNA synthesis is becoming cheap and routine, safe, scalable in-vivo delivery vectors remain a “black hole,” with telomerase gene therapy dismissed as a poor near-term target.  
- A concrete DIY RNAi protocol was outlined—synthesize a 25-bp DNA template, transcribe dsRNA, package into SNALP liposomes, and inject—acknowledging both feasibility and serious safety risks.  
- Participants emphasized that large-scale, low-cost DNA printing would accelerate brute-force functional screening and rational biological engineering, but the open-source microfluidic DNA-synthesis printer project stalled for lack of a chemist and incomplete chemistry design.  
- Deep-brain stimulation for depression was critiqued for high surgical morbidity (≈8 % intracranial bleed, 15 % infection, 6 mm brain shift), financial conflicts of interest among patent-holding investigators, and the suggestion to pursue non-invasive ultrasound neuromodulation instead.  
- A crowd-funded proposal to sequence the freeze-tolerant wood frog genome was floated to uncover molecular mechanisms permitting survival at –2 °C to –3 °C with circulatory arrest, potentially informing cryopreservation research.