- A long technical monologue (quoted by kanzure from the DIYbio list) explored DIY routes to reversible 3′-blocked dNTPs for low-cost enzymatic DNA synthesis, weighing chemoenzymatic sugar addition via disaccharide synthases, chemical phosphorylation of dirty ends, and enzymatic clean-up with PNKP, while conceding that none would beat the price or purity of commercial CleanAmp reagents.  
- kanzure shared two open-access papers: “Chemoenzymatic transformations in nucleoside chemistry” (a review of enzymatic routes to modified nucleosides) and “A two-neuron system for adaptive goal-directed decision-making in Lymnaea” (a Nature Communications study on minimal neural circuits for learning).  
- Hackteria’s DIY Micro Laser Cutter guide (linked by kanzure) was highlighted as a potentially useful tool for open-source bio-hardware fabrication.  
- Discussion of mind-mapping tools versus plain text for project scoping concluded that arbitrary cyclic graphs are essential for capturing complex interdependencies (e.g., emigrating to another country), but most software fails to support cycles, leading some participants to stick with text files and manual cross-references.  
- A brief aside linked to an interview with Dan Spitz, former Anthrax guitarist turned master watchmaker, illustrating the channel’s recurring interest in extreme career pivots and precision craftsmanship.