- N-acetylcysteine was highlighted as a compound that inhibits muscle fatigue in humans, with a supporting 1994 JCI paper provided.  
- Delta sleep-inducing peptide (DSIP) and the broader concept of “humoral transmission of sleep” were discussed, alongside skepticism about DSIP’s practical efficacy.  
- Optogenetic stimulation of the ventral tegmental area (VTA) dopamine neurons was noted to reanimate rodents from general anesthesia, with two independent papers (PNAS 2016 and Anesthesiology 2015) cited.  
- Whole-cortex electrophysiology combined with optogenetic tonic activation of the thalamic reticular nucleus (TRN) was shown to rapidly induce local, sleep-like slow-wave activity and reduced arousal in behaving animals.  
- A genome-wide association study in >119,000 individuals identified 13 morningness loci and 2 sleep-duration loci (notably PAX8 and VRK2), and these findings were added to the DIYH+ wiki’s genetic-modifications page.  
- The TNFα G308A polymorphism was added to the same wiki page as a candidate allele for resistance to sleep deprivation.  
- A proposal was floated to evolve megabase-scale genomes optimized for Gibson assembly inside single cells by iterative selection after transfecting ~10⁵ 100-mer oligos.  
- The hamster zona-free ovum (HZFO) test for human sperm function was explained, including the fact that successful penetration creates a “humster” hybrid embryo, prompting jokes about legal liability.  
- Discussion of the EM Drive paper centered on criticism that apparent thrust could be explained by thermal-expansion artifacts; participants argued for better controls such as on-resonance vs. off-resonance comparisons and a classic Cavendish torsion balance design.  
- Circadian disruption and cancer risk were linked, with a Medscape review and a 2012 Anticancer Research paper on melatonin’s role in cancer treatment shared as references.