- Open-source EEG hardware: discussion centered on the current state of low-cost, home-use EEG; OpenBCI (based on the TI ADS1299 24-bit ADC) was recommended, and participants debated whether EEG alone could provide region-specific biofeedback for stress-reduction training targeting the amygdala.  
- DIY brain-stimulation caution: ultrasound phased arrays were proposed as the only plausible non-invasive home method for targeted neuromodulation, but concerns were raised about hemorrhage risk, blood–brain-barrier disruption, and the chaotic micro-scale damage caused by cavitation jets.  
- Functional near-infrared spectroscopy (fNIRS) was suggested as a safer, more DIY-friendly alternative to both EEG and ultrasound for monitoring cortical activity.  
- Cryonics sidebar: Robert Falcon Scott’s 1912 Antarctic body (still entombed in ‑40 °C ice) was half-seriously cited as “the longest cryonic preservation” in response to a debate about historical claims.  
- DNA immunogenicity note: intramuscular plasmid DNA injections do not trigger anti-dsDNA antibodies in healthy mice but accelerate autoimmunity in autoimmune-prone strains, per a 2001 Nature Gene Therapy paper.  
- CAV-2 (canine adenovirus-2) vector highlighted as a potent, neuron-specific gene-delivery tool for neurobiology and potential transhumanist gene-therapy applications.  
- OpenMeca introduced: an open-source 3-D mechanical-system simulator built on Chrono::Engine, aimed at rapid prototyping of linkages, forces, and boundary conditions with built-in numerical sensors.  
- Directed-evolution automation critique: even leading labs still rely on room-sized robots shuttling thousands of 96-well plates; microfluidic miniaturization was urged as the next step to cut cost and labor.  
- Slides for an upcoming talk at the BEACON Center (Michigan) on “mad science and angry engineering” in directed evolution were shared; feedback included adding degenerate-synthesis terminology and references to Dawkins’ “blind watchmaker” concept.  
- Controversial biophysics hypothesis aired: superkuh argued that neuronal action potentials involve reversible lipid-bilayer phase changes driven by cation substitution, offering explanations for anesthetic pressure dependence and optical birefringence shifts.