- George Church and kanzure debate the future of sequencing: Church sees FISSEQ (fluorescent in-situ sequencing) as the next leap after nanopore, while kanzure stresses that FISSEQ’s positional data complements rather than replaces nanopore’s long reads; the only public genomic-FISSEQ disclosures are patent US20160304952 and pre-print biorxiv.org/055863, with no peer-reviewed reports yet.

- Open-hardware microscopy discussion compares DIY STM (~$1 k total cost, dberard.com guide) with piezo-electric cantilever read-out as an alternative to optical lever AFMs, referencing diyhpl.us AFM papers.

- The Odin’s $150 CRISPR bacterial education kit is endorsed by veteran DIYbio users as an effective, low-cost entry point for gene-editing practice.

- nmz787_ and others troubleshoot a home-built electroporator using The Odin’s $10 luminescent plasmid pVIB (pJE202); they conclude that immediate transformation of crude alkaline lysate is feasible if DH5α (not BL21) is used to avoid nuclease degradation, and that 5–6-year-old LB powder and sealed silica miniprep kits remain usable.

- A Medium article urges EU science policy to redirect public funds from traditional institutes to grassroots DIY labs; participants note the lack of concrete redistribution mechanisms.

- kanzure proposes a two-receptor co-localization chemical switch as a spatially precise targeted-drug-delivery system, activating therapeutics only where both receptors are present.

- Neuroscience links circulated include: single-cell allele-specific RNA-seq pre-print (biorxiv.org/109629); metformin impairing spatial memory and visual acuity in aged male mice; anterior-cingulate implant for alcohol-dependence treatment; vagus-nerve stimulation enhancing human memory; contagious mental effort; short-sleeper phenotype after third-ventricle decompression; and a possible mitochondrial mutation linked to chronic fatigue syndrome.