- **Brain-uploading resolution debate**: Participants estimated that reconstructing a brain for uploading would require imaging at synaptic-receptor and synaptic-weight resolution, plus full connectivity data; DNA barcoding was cited as a non-optical connectivity-mapping technique, while optical methods like FISSEQ and antibody labeling were noted for protein-level readout.  
- **DIY genetic modification notes**: Bryan Bishop added CDH13 rs11150556 (linked to verbal working-memory performance) to the diyhpluswiki genetic-modifications page, continuing the channel’s open catalog of human-gene targets.  
- **Aging-theory survey**: A newly shared patent application (WO2017062311) lists ten major historical theories of aging—from Metchnikoff’s 1901 phagocyte hypothesis to the 2012 redox-stress model—framing them as biological pathways to be reset therapeutically.  
- **Thymic rejuvenation reference**: A 1993 paper showing thymus restoration in aging mice via in-vivo zinc supplementation was circulated as a simple, low-tech anti-aging intervention.  
- **FIB acquisition discussion**: nmz787 and kanzure weighed purchasing a surplus Seiko SMI-3200 focused-ion-beam system (eBay listing ending the next day, starting bid $1,500), debating logistics, power draw (~1.7 kVA), and scrap-steel value if the deal fell through.  
- **Protein-interaction orientation tools**: nmz787 shared CMU’s OrientEdges algorithm and its open-source implementation “meo” (GitHub: agitter/meo) for inferring causal directionality in protein-interaction networks.  
- **State-sponsored eugenics example**: The 1935 Matanuska Valley Colony project—where the U.S. government selected northern-state farmers for an Alaskan settlement—was dissected as a flawed attempt at selective migration based on perceived hardiness.