- A participant reports having obtained milligram quantities of the FOXO4-DRI senolytic peptide used in Baar et al. 2017 and intends to self-experiment, prompting discussion on dosing and safety.  
- The channel circulated links to the Nature paper showing that human umbilical-cord plasma protein TIMP2 rejuvenates hippocampal function in aged mice, alongside the commercial NephroCheck TIMP2 kidney-injury test, raising questions about systemic effects.  
- kanzure highlighted two genetic constructs—INK-ATTAC and p16::3MR—that enable drug-inducible suicide-gene elimination of p16^Ink4a-positive senescent cells, noting upcoming combinatorial-multiplexing challenges for body-wide deployment.  
- A proposed alternative delivery scheme involves systemic mRNA/aptamer triggers that home to cells expressing a dedicated receptor, allowing whole-body epigenetic state flips after a single large dose followed by weeks of expression changes.  
- Links were shared to a 2017 review titled “The fountain of youth by targeting senescent cells?” and to Baker et al. 2011 and Demaria et al. 2014 as foundational senolytic-clearance studies.  
- Practical resources posted include a dose-translation guide for animal-to-human scaling and a Bio-Rad protocol for optimizing siRNA electroporation.  
- A new high-resolution whole-brain imaging method using oblique light-sheet tomography (bioRxiv 2017) was introduced as a potential tool for mapping senescent-cell distributions.  
- Discussion of mutantpedia centered on the difficulty of structuring phenotypic data collection and sourcing high-quality human mutants for an open genetic-variation database.  
- A brief aside explored sleep-state misperception and uni-hemispheric sleep in whales and dolphins as analogies for possible human “sleep-posting” phenomena.