- GP-write 2017 meeting livestream and transcripts: Bryan Bishop posted a full set of session-by-session transcripts covering Jef Boeke, George Church, Jackson Labs, octopus genome, plant phytodetectors, ethical frameworks, recombinase-mediated assembly, isothermal amplification arrays, and the “ultra-safe cell line” virus-resistance project.  
  – Links: http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2017-05-09/  
  – GP-write asked Bishop to stop tweeting unpublished material; public tweets at https://twitter.com/GP_write/status/861972050122080256 still highlight a citizen-science pilot using isothermal arrays for gene-length extension.

- George Church proposed a concrete synaptic barcoding protocol: use FISSEQ with antibodies targeted to pre- and post-synaptic clefts, then sequence both sides to recover paired barcodes even after tissue slicing.

- Technical paper shared: “High molecular weight DNA assembly in vivo for synthetic biology applications,” http://www.tandfonline.com/doi/pdf/10.3109/07388551.2016.1141394.

- Open discussion on oligo-array synthesis of sgRNA libraries and Cas9 “tetherings” as a scalable genome-editing toolkit.

- Funding note: Enevolv (directed-evolution startup) raised $1.79 M in 2014—link circulated as a benchmark for bio-startup finance.

- Human Metabolome Database (http://www.hmdb.ca/) surfaced in the context of engineering gut microbes for in-situ neurotransmitter production.

- Channel meta-commentary: frustration that “too many scientists, not enough engineers” dominate GP-write planning, with nanopore sequencing cited as an over-hyped cure-all.