- A single-molecule PCR protocol was described that can synthesize error-free DNA by repeatedly amplifying only perfectly overlapping fragments from a pool of error-containing oligos, trimming mismatched ends with exonuclease, and re-cycling until the full-length molecule is obtained (Nucleic Acids Research 36(17):e107, 2008).  
- Base-editing CRISPR systems (BE1) that fuse catalytically dead Cas9 to cytidine deaminase were highlighted for achieving ~23 % C→T (or G→A) correction in human embryos without double-strand breaks, with newer variants narrowing the editing window to 1–2 nt and relaxing PAM requirements (Nat. Commun. 7:13330, 2016).  
- A speculative “DNA tape drive” architecture was proposed in which a ring-shaped nanopore enzyme (e.g., modified TdT) incorporates a cytidine-deaminase domain and is electrically gated to step a DNA strand through and edit it base-by-base at millisecond timescales compatible with nanopore translocation.  
- Recent literature on electrically controllable protein conformation was collected, including voltage-driven IgG Fab and cytochrome-c conformational changes and magnetic-field modulation of transferrin, suggesting routes to voltage-controlled DNA-modifying enzymes (review: arXiv:1702.05028).  
- Nanopore-based data-storage concepts were floated, including using protein methyltransferases to write data as methylation marks on long filament proteins that are later read by nanopore threading, with electrode-functionalized “brakes” shown to slow ssDNA translocation to ~100 µs per base for reliable readout.  
- Single-molecule fluorescence studies revealed that dCas9 requires ~6 hours to locate its genomic target in E. coli, with off-target sites bound for <30 ms, prompting discussion of faster linear-scan enzymes or multiplexed search strategies.  
- The economics of large-genome synthesis were questioned, specifically George Church’s 2017 claim that whole-human-genome de-novo synthesis costs ~US$6 k, which participants noted is orders of magnitude below any publicly reproducible price.  
- Enzyme stability and directed-evolution strategies were discussed, focusing on evolving more thermostable polymerases, TdT, and recombinases via high-throughput DNA synthesis and error-prone PCR without sacrificing catalytic activity.