• A 1984 paper and its 2017 follow-up (doi:10.1038/nchembio.2192) describe an “energy-hijacking” side-pathway in plant Photosystem II; participants debate genetically blocking this route or diverting oxygen to raise ATP yield and are compiling a curated list of further photosystem-efficiency studies.

• The “pharmagarden” concept envisions transgenic plants engineered for high-yield pharmaceutical production, framed as a long-standing open-source biohacking dream.

• A single-molecule DNA-synthesis scheme is proposed: use nanopore electrophoresis to gate chain elongation so that an enzyme adds a nucleotide only when the nascent strand protrudes through the pore, followed by PCR amplification to reach usable quantities.

• Atomic-layer deposition (ALD) is discussed as a route to gas-phase enzymatic DNA synthesis—either by ALD-coating glycine or nucleotides onto surfaces with tethered TdT or by enabling spontaneous polymerization under vacuum.

• DIY automation tools shared include scripts that harvest PDF links from IRC logs and compile first-page thumbnails into yearly reading-list galleries (e.g., 2017-10-22-kanzure-reading-list-2017.v0.pdf) and a 9.5 MB 2017 literature archive re-hosted at diyhpl.us.

• Autodesk’s cloud-based Genetic Constructor CAD software for synthetic-biology design is announced via ACS Synthetic Biology, highlighting the trend toward open, browser-based bio-design platforms.

• A DIY human CRISPR gene-therapy how-to (diyhpl.us/wiki/transcripts/diy-human-gene-therapy-with-crispr/) and an intranasal cell-delivery paper are circulated, prompting discussion of administration routes and safety.

• Fecal microbiota transplantation is proposed as a DIY weight-control protocol—antibiotic pre-treatment followed by ingestion of “lean donor” stool—with Josiah Zayner’s biohacker protocol cited as reference.

• Newly detected intact lunar lava tubes at Marius Hills are floated as candidate sites for inflatable, lined Moon habitats, supported by resurfaced 1990s Oregon L5 Society maps and ISRU studies.

• Electrokinetic device design leverages conjugated-polymer electrodes (Wiley paper) to eliminate gas and pH by-products in electro-osmotic pumps, enabling low-voltage, printable lab-on-a-chip systems.