- CRISPR can be fused to meiotic proteins to drive targeted crossover events in plants, potentially increasing variation in clustered immunity genes (Deng et al., 2017).  
- A simple hypotonic-shock protocol achieves 46 % transgenesis efficiency in mice by injecting DNA directly into testis tubules, bypassing electroporation (S2329050117300359).  
- Magnetic nanoparticles conjugated to oocyte-specific histone H1 enable remote, fluorescent chromosome manipulation in oocytes, suggesting physical enucleation or repositioning without spindle removal.  
- Micromanipulation studies from the 1940-1960s showed that grasshopper chromosomes retain their meiotic behavior when transferred between spindles, implying chromosomes encode their own attachment and cohesion rules.  
- Continuous DNA synthesis “ticker-tape” feeding a ribosome-like molecular machine was proposed as a nanotech route to on-demand protein production, though no concrete application was identified.  
- Intranuclear microinjection routinely delivers 10⁻¹⁷ L volumes at 80–100 nuclei h⁻¹ in plant protoplasts, but single-oocyte scarcity makes low-efficiency steps prohibitive for human germline work.  
- Spermatogonial stem-cell extraction, genetic modification, and re-implantation can generate transgenic sperm, yet requires testicular irradiation to prevent mosaicism.  
- Envelope-free lentivirus injection into mouse zygotes yields high transgenesis rates, raising the question of whether naked DNA could achieve similar outcomes.  
- Interspecies blastocyst complementation and “reproductive semi-cloning” papers were reviewed for producing human gametes via chimeric animals, highlighting ethical and technical barriers.