- Discussion of CO₂ handling after condensation: participants considered re-evaporation to recycle latent heat but questioned what balloon or film material could prevent CO₂ diffusion for long-term storage.  
- Plant physiology note: high CO₂ is not the limiting factor for plant survival; instead, low O₂ becomes lethal in the dark when photosynthesis stops.  
- Open-source CAD tooling: FreeCAD 0.17 “Roland” released; Lulzbot is financially backing its development; assembly3 workbench and curated mech-eng resources were shared.  
- Bitcoin Lightning micropayments: “matreon” (GitHub: sjors/matreon) introduced as a self-hosted Patreon alternative using the Lightning Network.  
- Genome-synthesis scaling: Chemistry World article surveyed Twist Bioscience, Evonetix, Nuclera and others on how to “pump up the bases” for gene and whole-genome synthesis.  
- Extropianism outreach gap: participants lamented the absence of a concise, modern “hello from the future” introduction to extropian ideas and technology goals.  
- Polygenic embryo selection: gwern’s tweet-thread argued that, with the small embryo counts in IVF, even low-quality PGSes still yield meaningful selection gains; simulation plots provided.  
- Protein co-evolution hypothesis: citing 2001/2002 Nature & Science papers, participants proposed using population-wide covariance of protein variants to infer direct physical interactions and asked whether such a database already exists.  
- Mitochondrial–nuclear co-dependence: open questions on which genome encodes mitochondrial-membrane proteins, the evolutionary origin of the outer cell membrane, and why glycolysis (2 ATP) persists alongside oxidative phosphorylation (30 ATP).