- Discussion of low-cost DNA sequencing: an Instructables guide for building an Arduino-based PCR thermocycler for under $85 was shared as an entry-level tool for amplifying DNA prior to sequencing.  
- Trade-offs in sequencing methods were highlighted: participants noted that “best” approach depends on desired accuracy, coverage, and budget, with no single solution fitting all DIY or self-testing needs.  
- Sanger sequencing was identified as the standard follow-on technique after PCR for reading a few hundred base pairs at high accuracy, especially suitable for hobbyist or educational projects.  
- Motivations for DIY sequencing included (1) personal genotyping/self-testing and (2) gaining hands-on knowledge of the sequencing workflow.  
- A new transcript on “threshold signatures and accountability” from the 4 Feb 2019 SF Bitcoin Meetup was posted to the diyhpluswiki, expanding the channel’s archive of cryptographic research relevant to decentralized transhumanist infrastructure.