- **E-cigarette safety & DIY analytics**: The 2019 EVALI outbreak was traced to black-market THC cartridges adulterated with vitamin-E acetate (“Honey Cut”), underscoring the need for consumer-grade GC/MS, HPLC, IR/Raman spectroscopy, and electrical-impedance spectra to verify e-liquid composition and detect contaminants.  
- **Nicotine formulation trade-offs**: Nicotine salts (≈50 mg/mL) deliver higher concentrations with less throat hit and faster onset than harsher free-base nicotine, influencing both commercial products (e.g., Juul) and DIY mixing strategies.  
- **Ideoplast alphabet design**: A proposed symbol set must resist small deformations and symmetry operations (rotations, reflections); kanji partially meets these criteria, and a base58-like restriction scheme is suggested to avoid glyph collisions.  
- **Ethics of germline editing**: George Church’s “simultaneous universal access” criterion for human genetic enhancement was criticized as unrealistic; participants argued that early high cost is typical and that withholding technology in the name of equality is counter-productive.  
- **DIY serum cell culture**: Josiah Zayner’s experiment replacing fetal bovine serum with his own blood serum for cell culture was noted; while blood-borne pathogen concerns exist, small-scale, non-consumptive research use is considered low-risk.  
- **Nootropic re-evaluation**: Creatine is being reassessed for cognitive enhancement amid a flood of “snake-oil” products, with calls for first-hand efficacy data; phenibut offers cognitive benefits but carries unusual withdrawal risks, prompting off-label gabapentin tapering protocols.  
- **Non-invasive tissue ablation & DIY levitation**: High-intensity focused ultrasound (HIFU) / histotripsy was highlighted as a non-invasive ablation technique, while a shared YouTube tutorial “Portable Acoustic Tractor Beam: build it at your home” encourages DIY acoustic levitation hardware exploration.