- Discussion of Molecular Assemblies’ enzymatic DNA synthesis platform: they are outsourcing TdT protein-engineering to Codexis to improve incorporation fidelity and speed, and hold 24 patents on modified polymerase-based synthesis.  
- Forbes article and accompanying Codexis/Molecular Assemblies YouTube video highlighted as mainstream coverage of “next-gen DNA synthesis” using terminal deoxynucleotidyl transferase (TdT) and reversible terminator chemistries.  
- Nature Materials paper (lsneff link) on “de novo nanomaterial crystals from DNA frameworks” presented as an example of using DNA-origami scaffolds to grow inorganic nanocrystals with programmed shapes.  
- Debate on whether aptamer function requires high-entropy sequences or whether long, low-entropy repeats (100 bp stretches that switch pattern every few hundred bases) could still yield functional aptamers.  
- Proposal of “synthesis by sequencing-by-synthesis”: iteratively wash single dNTP species over a surface-tethered polymerase reaction and detect incorporation events in real time to build custom DNA strands.  
- Exploration of impedance-based or fluorescent single-molecule sensors for detecting nucleotide incorporation events, citing two 2019–2020 bioRxiv papers on cyclic reversible termination (CRT) enzymatic synthesis.  
- Observation that lowering temperature, reactant concentration, or adding Mn²⁺ can modulate polymerase kinetics and misincorporation rates, enabling slower, more controlled enzymatic synthesis.  
- Reference to ACS Synthetic Biology paper describing “universal template” CRT enzymatic synthesis that achieves stepwise yields of 98–99 % and could be scaled for on-chip DNA manufacturing.