- Early 20th-century rat maze-learning experiments (1924–1941) tracked cognitive performance across 16 generations, revealing non-linear inheritance patterns and prompting questions about single-trait versus multi-trait selection metrics.  
- Discussion of aldehyde-stabilized cryopreservation (ASC) clarified that glutaraldehyde cross-links proteins to resist osmotic damage during cryoprotectant perfusion, while osmium tetroxide remains necessary for lipid staining despite its toxicity and disposal challenges.  
- Participants debated the feasibility of post-mortem aldehyde perfusion, noting that fixation speed, blood-clot viscosity, and the “no-reflow” phenomenon are larger practical barriers than rapid vascular sealing.  
- A new GitHub-hosted wiki on chemopreservation (https://github.com/ultrastructural-preservation/chemopreservation/wiki) was highlighted as a community resource covering hydrogel and non-cryogenic brain-preservation techniques.  
- DNA nanoscopy (Nature 2017, https://www.nature.com/articles/s41467-017-00542-3) was presented as a method to obtain sub-micron connectomic and biomolecular data via DNA barcoding and sequencing, complementing antibody or aptamer labeling approaches.  
- Longevity genetics content was updated on diyhpluswiki with the SNP rs2146881, and the channel’s Discord bridge experiment continued despite complaints about vendor lock-in and mobile-client UX issues.  
- Speed-breeding protocols achieving 3–9 plant generations per year and 140-generation mouse selection for litter size were shared as examples of accelerating artificial selection beyond traditional timelines.  
- Cost analysis of caffeine biosynthesis versus extraction showed bulk caffeine powder at ~US$60 kg⁻¹ versus retail ground coffee at a 5× markup, prompting speculation about engineered microbial production.