- A new CRISPR-based “PASTE” system (Programmable Addition via Site-Specific Targeting Elements) was highlighted: it fuses a Cas9 nickase to a reverse transcriptase and a serine integrase to insert kilobase-scale DNA sequences without creating double-strand breaks, leaving only short att-site scars (US patent 11,572,556; Nature Protocols 2022).  
- wildDISCO whole-body fluorescent mouse atlas and X-ray holographic nano-tomography (XNH) at synchrotrons were discussed as cutting-edge imaging tools—offering sub-100 nm resolution over millimetre-scale samples and the long-term hope of in-vivo use if radiation dose can be reduced.  
- Participants debated the practicality of neutrino tomography for biological imaging; consensus was that interaction cross-sections and detector size make it infeasible for tissue contrast, though mobile van-scale detectors already track nuclear warheads.  
- A “Wolverine” prototype was noted—an AI wrapper that reruns crashing Python scripts while GPT-4 iteratively edits and explains the fixes—praised as clever but flagged for its obvious security and oversight risks.  
- Open questions were raised about prospects for deep-tissue adult gene therapy: AAV capsid engineering (firms named: DynoTx, Capsidia, Voyager, 4DMT, Apetura, LogicBio, Capsigen) is targeting muscle, heart and brain, with excitement around programmable B-cell biologics for on-demand therapeutic production.  
- An ultra-cheap PCR clean-up protocol using PEG/salt precipitation was shared (scifind.io post) as a low-cost DIY alternative to spin columns.