- exa.ai/search was praised as an effective, clean-interface search engine for technical queries.  
- Recent papers challenge the “born with all eggs” dogma: postnatal oogenesis reported in humans, and functional oocytes were generated from male-mouse iPSCs, enabling male-to-female gamete conversion.  
- Multiple in-vitro gametogenesis strategies were discussed: (a) mitotic expansion of mature oocytes via cell-cycle regulators followed by re-arrest at MII, (b) direct reprogramming of diploid somatic cells into haploid oocytes, (c) derivation of haploid embryonic stem cells from parthenogenetic embryos by microsurgical removal of the male pronucleus, and (d) nuclear transfer of such haploid ESCs into donor oocytes.  
- Ethical and practical sourcing of human oocytes was debated: proposals included harvesting millions of oocytes from aborted embryos or using interspecies blastocyst complementation; legal and epigenetic concerns were raised.  
- Links to key papers and resources:  
  – “Oocyte formation by mitotically-active germ cells purified from ovaries of reproductive-age women” (PMC3296965)  
  – “Generation of functional oocytes from male mice in vitro” (Nature 2023)  
  – “Induction of somatic cell haploidy by premature cell division” (Sci. Adv.)  
  – “In vitro expansion of human sperm through nuclear transfer” (PMC7118075)  
  – LessWrong post on “twiblings” and four-parent babies.  
- diyhpl.us wiki commits added transcripts and notes on fertility upregulation, Bitcoin-dev mailing-list mirrors, and fixes for link rot affecting historical transcripts.