--- Log opened Mon Feb 20 00:00:06 2017
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01:21 < nmz787_> ah ha, found the missing pipette (and a label maker I'd just given up hope for a few weeks ago, when I ordered a new label maker, albeit with USB this time)
01:22 < nmz787_> though it is a low-range 0.5-5 uL
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05:20 < kanzure> https://soundcloud.com/bitcoincore/interview-with-jonas-schnelli
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07:07 < kanzure> https://www.certificate-transparency.org/log-proofs-work
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07:50 < kanzure> "Large-scale analysis of CRISPR-cas9 cell-cycle knockouts reveals the diversity of p53-dependent responses to cell-cycle defects" http://www.cell.com/developmental-cell/fulltext/S1534-5807(17)30039-4
07:52 < kanzure> from https://twitter.com/iaincheeseman/status/832775350530633728
08:04 < kanzure> "Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis" http://www.nature.com/nprot/journal/v12/n3/full/nprot.2016.165.html
08:06 < kanzure> hrm http://git.osmocom.org/
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08:26 < kanzure> .wik AR-R17779
08:26 < yoleaux> "AR-R17779 is a drug that acts as a potent and selective full agonist for the ?7 subtype of neural nicotinic acetylcholine receptors. It has nootropic effects in animal studies, but its effects do not substitute for those of nicotine. It has also recently been studied as a potential novel treatment for arthritis." -- https://en.wikipedia.org/wiki/AR-R17779
08:39 < kanzure> wonder how bad  viruses are at targeting neuronal subtypes. i guess you could use lots of expression-gated constructs as tests to see what neuron you're being expressed in.
08:42 < kanzure> and whether we have the data on what gets expressed for all the umpteen zillion cell differentiation types
08:43 < kanzure> oh right, it's not enough to know what subtype you are because what matters more is the neighborhood. hmm. well, maybe you could have viruses with cas9 payloads and the payloads only activate in the presence of ultrasound/magnetic stimulation which you could localize to a specific brain target area.
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09:52 < kanzure> hgp-write (human genome project) meeting may 2017 registration is open http://engineeringbiologycenter.org/may-2017-meeting/
10:03 < kanzure> boom registered to attend
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10:37 < kanzure> http://engineeringbiologycenter.org/wp-content/uploads/2016/12/GP-Write-WhitePaper.pdf
10:37 < kanzure> hgp-write videos from 2016 https://www.youtube.com/playlist?list=PLHpV_30XFQ8R2Kpcc1pwwXsFnJOCQVndt
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11:00 < chris_99> https://www.fraunhofer.de/en/press/research-news/2017/february/app-reveals-constituents.html -- does anyone understand how that works, i'm really struggling to understand how, with just visible light shone onto an apple, using the camera, you can detect pesticides
11:04 < chris_99> like it doesn't mention anything about any hardware modification like grating etc.
11:09 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=f5e8c28e Bryan Bishop: hgp-write 2016: overview >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/overview/
11:12 < kanzure> :( "So where do we start with this? As I've said, we're talking about a cell line, engineered for safety, this would be presumably an IPS cell so that it would have the capability of being differentiable into different types of tissues, and it would be engineered to have a strict germline firewall. For example, removal of one of the sex chromosomes, an XO cell line, would be a simple way to en...
11:12 < kanzure> ...sure that this would not be able to become a germline. These cells can be differentiated into tissues and organoids, models of human organs, to evaluate the functional effects of any designer changes that are introduced."
11:12 < kanzure> how are they going to stop you from synthesizing the other chromosomes?
11:12 < kanzure> why would they want to stop you from synthesizing the other chromosomes, too?
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12:16 < nmz787_> chris_99: pretty neat, that fraunhofer thing
12:16 < chris_99> yeah i think i get it roughly now, but don't see the advantage over a grating
12:17 < nmz787_> chris_99: seems like it is just generally using the display as a monochrome photon source, and using the camera as a 3 channel detector
12:17 < chris_99> mm and cycles through different colours
12:17 < nmz787_> and using algorithms in between to extract as much information from the limited data
12:17 < nmz787_> yeah
12:18 < chris_99> it seems very clever, but yeah i don't get why you'd use it over a normal vis spectrometer
12:18 < nmz787_> well as they said, they can take advantage of the millions/billions of existing devices
12:18 < chris_99> mm true
12:18 < nmz787_> any RGB camera it basically is a 3 channel spectrometer
12:19 < chris_99> i think i may have asked this before, but is there a decent open spectral db you could reference
12:20 < nmz787_> PLOTS has something, but I don't know if it is decent quality
12:20 < chris_99> oh yeah i've seen that, but yeah i dunno about the quality either
12:20 < nmz787_> my guess is some of their data is better quality than other
12:21 < nmz787_> (I mean mixed quality, some users submitting great stuff, others subpar)
12:21 < chris_99> mm
12:21 < JayDugger> nn
12:21 < chris_99> wait you do mean this right - https://spectralworkbench.org/
12:22 < nmz787_> yeah
12:22 < JayDugger> That's interesting, thanks.
12:23 < nmz787_> from what I remember about the Raman spectrometers that i.e. govt/security agencies use, the database is a MAJOR part of what you pay for as a user
12:24 < nmz787_> or at least that was my gut feeling, based on what they were selling the hardware for
12:24 < chris_99> ahh
12:24 < chris_99> pity
12:24 < nmz787_> (which is of course very specialized and not-very-high-volume manufacturing... but still seemed 10 to 20X less from my personal development estimates)
12:25 < nmz787_> I mean my estimated BOM was 10-20X less than they sales price of the commercial units
12:25 < chris_99> ah interesting, did you start making one?
12:25 < nmz787_> now, maybe I was not considering all the engineering cost that went in over years of work
12:25 < nmz787_> vs just BOM
12:27 < chris_99> https://www.alibaba.com/product-detail/High-pressure-high-temperature-BARS-system_113116102.html thought that was interesting, i wonder how long it takes to pay back the ~$150k with synthetic diamonds
12:27 < nmz787_> chris_99: mostly was just planning... worked on that spectrometer CCD board, researched what optics seemed ideal, found manufacturer in China for the combo curved-grating, found filters, found some light sources
12:27 < chris_99> gotcha, i remember you saying how expensive the gratings are i think
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12:55 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8d99c04f Bryan Bishop: hgp-write: synthetic yeast genome project lessons learned >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/synthetic-yeast-genome-project-lessons-learned/
12:55 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=1ecde6da Bryan Bishop: hgp-write transcript: george church >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/from-recoli-to-1-million-genomes/
12:55 < kanzure> http://diyhpl.us/wiki/transcripts/hgp-write/2016-05-10/from-recoli-to-1-million-genomes/
12:56 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=7059606a Bryan Bishop: fix minor typo >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/from-recoli-to-1-million-genomes/
12:56 < kanzure> "If we go forward to today, we have 8 major tools for genome engineering and editing, including group 2 introns, cas9, meganuclease, recombinase, ZnF, ALE, recA CAGE, and lambda bet/exo MAGE"
12:56 < kanzure> "We have crystal structures for most of these, but not for lambda beta to my dismay"
12:58 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=474036f6 Bryan Bishop: another typo, this time about TALE >> 
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13:27 < chris_99> nmz787_, you have one of those cube laser engraver things don't you?
13:27 < nmz787_> yeah
13:27 < chris_99> i'm wondering, given enough passes if you cut cut expoxy of an IC with one
13:28 < nmz787_> was planning to change the laser to a blu-ray drive optical sled/module... which I bought with a controller circuit from the top of this page http://www.diyouware.com/store
13:28 < nmz787_> hmm
13:28 < nmz787_> chris_99: can you give an example of epoxy on an IC? I mean, how do I identify one as such, from the ICs I have laying around
13:29 < nmz787_> just the generic black plastic most chips look like?
13:29 < chris_99> i mean any IC they generally use epoxy afaik, try a 555 timer or something if you can
13:29 < chris_99> i was thinking if you can use for ICs i might get one, as i have microscope already
13:29 < nmz787_> mmm
13:29 < kanzure> this one? http://www.the-odin.com/plasmid-pje202-pvib-to-create-bioluminescent-bacteria/
13:30 < nmz787_> ok, I need to build a wooden work bench for the next at least hour... so I can setup some biolab equipment I pulled out of my garage
13:30 < nmz787_> but I will try to get that done this week chris_99
13:30 < chris_99> cheers, good luck with your wood work
13:30 < nmz787_> kanzure: yeah that is the one I ordered
13:30 < kanzure> typo on that page 'bioluminescience'
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13:33 < chris_99> nmz787_, https://www.youtube.com/watch?v=FGa3xfVPwGA they wrote some text on one heh
13:39 < nmz787_> chris_99: cool! is that easier than chemical etching?
13:40 < nmz787_> chris_99: I have some violin rosin that someone long ago said worked as an etchant
13:40 < chris_99> i do have fuming nitric, but no hot plate
13:40 < nmz787_> chris_99: http://hackaday.com/2010/07/16/decapping-integrated-circuits-with-sap/
13:41 < chris_99> i'm very tempted now heh, they're like ?57 apparently for a 1W one
13:41 < nmz787_> that's $74... with shipping ?
13:42 < nmz787_> I think I paid $100 or $120 with shipping
13:42 < chris_99> www.ebay.co.uk/itm/NEJE-DK-8-KZ-High-Power-Laser-Engraver-Printer-Machine-1000mW-1000MW-/282303551315 shipping is free
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13:44 < nmz787_> chris_99: the button on top is something mine lacks
13:44 < chris_99> heh is that like an emergency stop thing
13:44 < nmz787_> chris_99: with the mention of an SD card... I wonder if it has some offline/not-attached-to-PC mode... though they don't annotate a SD slot in their pics
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13:45 < nmz787_> "Can be used offline, even if leave the computer,only connect the two USB ports to charger or power bank (5V 1A)."
13:46 < chris_99> what's a TF card?
13:47 < chris_99> oh sounds like it means microsd
13:48 < nmz787_> yeah
13:48 < nmz787_> chris_99: this might be a few pounds cheaper, looks like it is cheaper in USD http://www.gearbest.com/3d-printers-3d-printer-kits/pp_343187.html
13:48 < nmz787_> says free shipping to UK
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13:49 < chris_99> cheers
13:53 < chris_99> there seems some on taobao ever cheaper actually
13:53 < chris_99> *even
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13:59 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=2d0e184f Bryan Bishop: hgp-write transcript: ultra-safe cell lines >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/ultra-safe-cell-line/
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14:17 < archels> grr
14:17 < archels> any good sources for 0.05" (1.27 mm) pitch prototype PCBs?
14:29 < kanzure> https://scotthelme.co.uk/csrf-is-dead/
14:30 < kanzure> http://engineeringbiologycenter.org/wp-content/uploads/2016/10/HGP-write-May-Meeting-Summary.pdf
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15:06 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=ec7a2e67 Bryan Bishop: add ultra-safe cell line diagram >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/ultra-safe-cell-line/
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15:44 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=ac8c007d Bryan Bishop: hgp-write transcript: essential vitamins into human genome >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/ultra-safe-cell-line/
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16:07 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8f07a298 Bryan Bishop: hgp-write transcript: stem cell differentiation >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/seven-signals-toolbox-cell-tissue-therapies/
16:08 < kanzure> that was a really good talk.
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16:29 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=8d563231 Bryan Bishop: hgp-write transcript: recoding ecoli to 57 codons >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/recoding-ecoli-to-57-codons/
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17:08 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=6f95cff2 Bryan Bishop: hgp-write transcript: xenotransplantation >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/transplantation/
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17:48 < gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=7533933b Bryan Bishop: hgp-write: haploid embryonic stem cells >> http://diyhpl.us/diyhpluswiki/transcripts/hgp-write/2016-05-10/haploid-embryonic-stem-cells/
17:50 < kanzure> "Sprites: Payment channels that go faster than lightning" https://arxiv.org/abs/1702.05812
17:52 < kanzure> according to above presentation, if you leave human haploid cells alone in a tissue culture long enough, they become diploid
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19:10 < kanzure> hrm
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19:56 < kanzure> .wik moyasimon
19:56 < yoleaux> "Moyasimon: Tales of Agriculture, known in Japan as Moyashimon (??????), is a Japanese manga series by Masayuki Ishikawa. It was serialized in Kodansha's seinen magazine Evening from August 2004 to June 2013 and moved to the magazine Morning two, where it concluded in January 2014." -- https://en.wikipedia.org/wiki/Moyasimon
19:56 < kanzure> "The series follows Tadayasu Sawaki, a first-year college student at an agricultural university, who has a unique ability to see and communicate with bacteria and other microorganisms."
19:58 < cluckj> it is a weird show, but totally watchable
20:04 < adlai> http://ase.tufts.edu/cogstud/assets/searle.jpg
20:05 < kanzure> adlai: searle is a waste of time
20:06 < adlai> did you see the image or just go by the filename?
20:07 < adlai> how about i supply the text that linked to it: ""The shortest, funniest refutation of Searle's Chinese Room""
20:07 < kanzure> for shortest they should have just used the last pane though
20:08 < cluckj> I got searle to autograph derrida's limited, inc.
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20:09 < cluckj> conned really, but that was kind of the idea
20:26 < kanzure> something about flies http://www.columbia.edu/cu/alumni/Magazine/Legacies/Morgan/ https://news.ycombinator.com/item?id=13692166
20:27 < kanzure> .title http://www.bionet.ee.columbia.edu/hackathons/ffbh/2017
20:27 < yoleaux> Fruit Fly Brain Hackathon 2017 | Bionet
20:27 < cluckj> that looks cool, I need to get back into the research game :\
20:31 < kanzure> you think this is a game? https://www.youtube.com/watch?v=493ljyoox6o
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22:12 < nmz787_> archels: oshpark doesn't do that?
22:14 < nmz787_> archels: oshpark says they do 0.154mm pitch and line width minimums
22:14 < nmz787_> archels: http://docs.oshpark.com/services/two-layer/
22:15 < nmz787_> archels: even smaller at 0.127 mm for 4-layer boards http://docs.oshpark.com/services/four-layer/
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--- Log closed Tue Feb 21 00:00:39 2017