--- Log opened Mon Apr 22 00:00:51 2019 00:19 -!- hehelleshin [~talinck@98.29.27.253] has joined ##hplusroadmap 00:23 -!- Guest72969 [~talinck@98.29.27.253] has quit [Ping timeout: 246 seconds] 00:47 -!- LeoTal [~Adium@62.247.7.93.rev.sfr.net] has joined ##hplusroadmap 00:48 -!- LeoTal1 [~Adium@63.247.7.93.rev.sfr.net] has joined ##hplusroadmap 00:51 -!- LeoTal [~Adium@62.247.7.93.rev.sfr.net] has quit [Ping timeout: 245 seconds] 01:17 -!- l_wl [~l_wl@pool-108-45-141-95.washdc.fios.verizon.net] has quit [Ping timeout: 244 seconds] 01:17 -!- l_wl [~l_wl@pool-108-45-141-95.washdc.fios.verizon.net] has joined ##hplusroadmap 04:08 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 06:49 -!- superkuh_ [~superkuh@2601:282:b01:a890::2f3d] has joined ##hplusroadmap 07:05 < kanzure> https://hackernoon.com/python-3-7s-new-builtin-breakpoint-a-quick-tour-4f1aebc444c 07:14 -!- superkuh_ [~superkuh@2601:282:b01:a890::2f3d] has quit [Quit: Leaving] 07:48 -!- yashgaroth [~yashgarot@2606:6000:ca81:3100:9018:9211:5c11:660b] has joined ##hplusroadmap 08:08 -!- strages [uid11297@gateway/web/irccloud.com/x-zncslgfltwaqvoht] has joined ##hplusroadmap 09:21 -!- superkuh_ [~superkuh@67.133.97.101] has joined ##hplusroadmap 09:26 -!- superkuh__ [~superkuh@67.133.97.101] has joined ##hplusroadmap 09:26 -!- superkuh_ [~superkuh@67.133.97.101] has quit [Read error: Connection reset by peer] 09:27 -!- superkuh__ [~superkuh@67.133.97.101] has quit [Remote host closed the connection] 10:30 < fenn> https://news.berkeley.edu/2019/03/28/introducing-a-gentler-way-to-blow-holes-in-cells/ 10:30 < fenn> from the same lab https://newscenter.lbl.gov/2017/09/18/copper-catalyst-yields-high-efficiency-co2-fuels-conversion/ 10:31 < kanzure> .tw https://twitter.com/biohackinfo/status/1119223060614262785 10:31 < yoleaux> Patent battles have turned Doudna into a crafty farsighted mastermind. When ethics debates get old and moratoriums become outmoded, Doudna and her biotech enterprises will be the ones to gain the most from the CRISPR babies fiasco. Doudna is playing 4D chess with everyone. 👏 https://pbs.twimg.com/media/D4hHcRXUUAEygFn.jpg (@biohackinfo) 10:33 < fenn> "nanoEP should theoretically work with any type of cell, delivering macromolecules, such as Cas9, into an embryo, for example, or altering human T-cells for immunotherapy. And the delivery method does not require specialized equipment or buffer solutions." with 80% efficiency 10:34 < kanzure> oh this is because the surface area of a cell sitting on a nanopore water filter is pretty minimal 10:35 < kanzure> "Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules" https://www.pnas.org/content/116/16/7899 10:35 < fenn> @biohackinfo is full of crap. doudna is just getting swept along in the fervor 10:37 < fenn> ahaha 10:37 < fenn> .tw https://twitter.com/biohackinfo/status/1118532732861190144 10:37 < yoleaux> Quiet honestly the term "biohacking" might already be lost to the butter-in-coffee snakeoil shills who've rebranded crap from the "wellness" and fitness industries as 'biohacking.' The pseudo-biohackers have won. #RIPbiohacking #ThoughtsAndPrayers https://pbs.twimg.com/media/D4XTlPbU8AAAgiv.jpg https://pbs.twimg.com/media/D4XTlf7U0AA8_0C.jpg https://pbs.twimg.com/media/D4XTlxKVUAEH7zZ.jpg (@biohackinfo) 11:44 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has joined ##hplusroadmap 12:31 -!- NikopolSohru [~Nikopol_@185.180.222.179] has joined ##hplusroadmap 12:33 -!- NikopolSohru [~Nikopol_@185.180.222.179] has quit [Changing host] 12:33 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has joined ##hplusroadmap 12:50 -!- NikopolSohru [~Nikopol_@unaffiliated/nikopolsohru] has quit [Quit: Leaving] 13:15 -!- LeoTal1 [~Adium@63.247.7.93.rev.sfr.net] has quit [Quit: Leaving.] 13:52 -!- dongcarl [sid321684@gateway/web/irccloud.com/x-ghdbduhtbgdfeyoq] has quit [Read error: Connection reset by peer] 13:53 -!- potatope [sid139423@gateway/web/irccloud.com/x-zdkiejqbtgxeounm] has quit [Ping timeout: 252 seconds] 13:53 < kanzure> "Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq" https://science.sciencemag.org/content/364/6437/286.full 13:55 < kanzure> "Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). Wienert et al. followed the recruitment of a DNA repair protein to DNA breaks induced by CRISPR-Cas9, enabling unbiased detection of off-target editing in cellular and animal models. Zuo et al. identified off-targets without the interference of natural genetic ... 13:55 < kanzure> ...heterogeneity by injecting base editors into one blastomere of a two-cell mouse embryo and leaving the other genetically identical blastomere unedited. Jin et al. performed whole-genome sequencing on individual, genome-edited rice plants to identify unintended mutations." 13:58 < kanzure> "We developed DISCOVER-Seq (discovery of in situ Cas off-targets and verification by sequencing), a universally applicable approach for unbiased off-target identification that leverages the recruitment of DNA repair factors in cells and organisms. Tracking the precise recruitment of MRE11 uncovers the molecular nature of Cas activity in cells with single-base resolution. DISCOVER-Seq works ... 13:58 < kanzure> ...with multiple guide RNA formats and types of Cas enzymes, allowing characterization of new editing tools. Off-targets can be identified in cell lines and patient-derived induced pluripotent stem cells and during adenoviral editing of mice, paving the way for in situ off-target discovery within individual patient genotypes during therapeutic genome editing." 13:58 < kanzure> in other words, 13:58 < kanzure> like i had said earlier, the solution to off-target effects is lots and lots of sequencing 13:59 -!- arisum [sid283163@gateway/web/irccloud.com/x-dlpaauissgmmfzvy] has quit [Ping timeout: 252 seconds] 14:07 -!- potatope [sid139423@gateway/web/irccloud.com/x-hknlwyzalmumjmrr] has joined ##hplusroadmap 14:08 -!- arisum [sid283163@gateway/web/irccloud.com/x-mxkokkxwaekyiryf] has joined ##hplusroadmap 14:08 -!- dongcarl [sid321684@gateway/web/irccloud.com/x-ijjocadrclzhpudm] has joined ##hplusroadmap 14:12 -!- N-time [~Mark@212.225.170.16] has joined ##hplusroadmap 14:15 -!- adamsky [~adamsky3@178235181086.unknown.vectranet.pl] has joined ##hplusroadmap 14:56 -!- Gurkenglas_ [~Gurkengla@unaffiliated/gurkenglas] has joined ##hplusroadmap 14:58 -!- bbrittain [~bbrittain@ec2-34-242-76-124.eu-west-1.compute.amazonaws.com] has joined ##hplusroadmap 14:58 -!- bwb_ [~bbrittain@ec2-34-242-76-124.eu-west-1.compute.amazonaws.com] has quit [Ping timeout: 246 seconds] 15:00 -!- Gurkenglas [~Gurkengla@unaffiliated/gurkenglas] has quit [Ping timeout: 246 seconds] 15:01 -!- arisum [sid283163@gateway/web/irccloud.com/x-mxkokkxwaekyiryf] has quit [Ping timeout: 246 seconds] 15:04 -!- arisum [sid283163@gateway/web/irccloud.com/x-vyeyabzqpjosmqgj] has joined ##hplusroadmap 15:07 -!- dongcarl [sid321684@gateway/web/irccloud.com/x-ijjocadrclzhpudm] has quit [Ping timeout: 276 seconds] 15:11 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has quit [Ping timeout: 250 seconds] 17:19 -!- gnusha [~gnusha@unaffiliated/kanzure/bot/gnusha] has joined ##hplusroadmap 17:19 -!- Topic for ##hplusroadmap: biohacking, nootropics, transhumanism, open hardware | as investigated by the fbi weapons of mass destruction division | this channel is LOGGED: http://gnusha.org/logs | http://diyhpl.us/wiki 17:19 -!- Topic set by kanzure [~kanzure@unaffiliated/kanzure] [Thu Feb 14 19:18:41 2019] 17:19 [Users ##hplusroadmap] 17:19 [ adlai ] [ dustinm ] [ jrayhawk ] [ MarkOtaris ] [ petercommand] [ srk ] 17:19 [ AgenttiX ] [ EnabrinTain] [ juri__ ] [ mgxm ] [ pompolic ] [ strages ] 17:19 [ andytoshi ] [ ensign ] [ justanotheruser] [ midnightmagic] [ potatope ] [ streety ] 17:19 [ archels ] [ fenn ] [ juul ] [ mnemon ] [ preview ] [ superkuh ] 17:19 [ arisum ] [ gnusha ] [ kanzure ] [ mrdata ] [ rancyd ] [ t4nk ] 17:19 [ balrog ] [ gwillen ] [ kuldeep ] [ namespace ] [ rancyd_ ] [ Taek ] 17:19 [ bbrittain ] [ heath ] [ kun0[m] ] [ nanotube ] [ rodarmor ] [ TMA ] 17:19 [ berndj ] [ hehelleshin] [ l_wl ] [ nickjohnson ] [ s0ph1a ] [ traumschule] 17:19 [ cannedprimates] [ HEx1 ] [ Livestradamus ] [ nmz787 ] [ saurik_ ] [ Urchin ] 17:19 [ cluelessperson] [ Hooloovo0 ] [ LooCfur ] [ nsh ] [ SDr ] [ yashgaroth ] 17:19 [ Cory ] [ indiebio ] [ Madars ] [ pasky ] [ sivoais ] [ yoleaux ] 17:19 [ dentist ] [ Jay_Dugger ] [ Malvolio ] [ pepesza ] [ Solgriffin__] [ yorick ] 17:19 [ dongcarl ] [ Jenda` ] [ mark_otaris ] [ periffis ] [ spaceangel ] 17:19 -!- Irssi: ##hplusroadmap: Total of 77 nicks [0 ops, 0 halfops, 0 voices, 77 normal] 17:19 -!- Channel ##hplusroadmap created Thu Feb 25 23:40:30 2010 17:20 -!- Irssi: Join to ##hplusroadmap was synced in 40 secs 17:30 -!- spaceangel [~spaceange@ip-86-49-16-65.net.upcbroadband.cz] has quit [Remote host closed the connection] 17:36 -!- Urchin [~urchin@unaffiliated/urchin] has quit [Read error: Connection reset by peer] 18:18 -!- strages [uid11297@gateway/web/irccloud.com/x-zncslgfltwaqvoht] has quit [Quit: Connection closed for inactivity] 19:16 < jrayhawk> i find it funny that the cultural dilution caused by popular usage of "lifehack" and "biohack" get nerds more upset these days than the semantic drift caused by popular usage of "hacker" does 19:21 < jrayhawk> i suppose this is why nerds hate XKCD, too. anything that makes the subculture too accessible is an existential threat to the subculture's continued existence as such 19:36 -!- l_wl [~l_wl@pool-108-45-141-95.washdc.fios.verizon.net] has quit [] 19:46 -!- king_mamba [~king@8ta-250-4-99.telkomadsl.co.za] has joined ##hplusroadmap 19:47 < king_mamba> hi 19:56 -!- king_mamba [~king@8ta-250-4-99.telkomadsl.co.za] has quit [Quit: Leaving] 20:04 -!- LeoTal [~Adium@156.170.86.79.rev.sfr.net] has joined ##hplusroadmap 20:07 < kanzure> "The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration" https://www.nature.com/articles/s41467-019-09746-1 20:21 < nmz787> fenn: that nanoEP thing seems obvious to me, having studied electroporation books from the early days of the technique being espoused... it's always been about Volts/distance (field strength) 20:23 < nmz787> kanzure: I don't think it has to do with the surface of the cell sitting on the nanopore, rather I think it just is that the nanopore screens the electric field from being distributed over the entire surface of the cell 20:27 < nmz787> https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4141-4 20:27 < nmz787> .title 20:27 < yoleaux> Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes | BMC Genomics | Full Text 20:27 < nmz787> "We performed matrix multiplication between all possible 3-mer sequences (N = 64)" 20:28 < nmz787> basically by using 3 primers per molecule, they can make a sort of multiplexed tag 20:36 -!- yashgaroth [~yashgarot@2606:6000:ca81:3100:9018:9211:5c11:660b] has quit [Read error: Connection reset by peer] 20:36 -!- yashgaroth [~yashgarot@2606:6000:ca81:3100:9018:9211:5c11:660b] has joined ##hplusroadmap 20:37 < nmz787> so i.e. even though a unique set of 3-mers has 64 oligos, if you use 3 3-mers in series (separated by a bit) the effect is multiplicative (4^3 * 4^3 * 4^3)== 262144 20:37 < nmz787> which is not that non-obvious, but w/e 20:55 -!- periffis_ [~g@66.205.193.233] has joined ##hplusroadmap 20:58 -!- periffis [~g@66.205.193.233] has quit [Ping timeout: 255 seconds] 21:08 -!- periffis_ is now known as periffis 22:27 -!- ebowden [~ebowden@unaffiliated/ebowden] has joined ##hplusroadmap 22:49 -!- TC [~talinck@98.29.27.253] has joined ##hplusroadmap 22:49 -!- TC is now known as Guest45662 22:53 -!- hehelleshin [~talinck@98.29.27.253] has quit [Ping timeout: 255 seconds] 23:02 -!- yashgaroth [~yashgarot@2606:6000:ca81:3100:9018:9211:5c11:660b] has quit [Quit: Leaving] --- Log closed Tue Apr 23 00:00:52 2019