--- Log opened Fri Sep 19 00:00:44 2025
01:27 -!- A_Dragon [A_D@libera/staff/dragon] has joined #hplusroadmap
01:32 -!- gAy_Dragon [A_D@libera/staff/dragon] has quit [Ping timeout: 624 seconds]
03:47 < hprmbridge> kanzure> an IQ testing company that also publishes pages on genetic enhancement and links to lesswrong? https://www.riotiq.com/articles/studying-iq-matters kind of unusual.
03:49 -!- WizJin_ [~Wizzy@150.129.166.149] has quit [Read error: Connection reset by peer]
03:49 -!- WizJin_ [~Wizzy@2402:a00:184:8354:e543:2733:1fa1:9bc5] has joined #hplusroadmap
03:51 < hprmbridge> kanzure> why were we okay with electroshock therapy to try to fix depression, but somehow brain surgery to fix stupidity is off limits?
04:11 -!- sivoais_ [~zaki@199.19.225.239] has quit [Ping timeout: 260 seconds]
04:11 -!- sivoais [~zaki@199.19.225.239] has joined #hplusroadmap
04:17 < fenn> brain surgery to fix stupidity?
04:18 < fenn> they do stuff like drilling holes and installing shunts to relieve intracranial pressure, which i suppose would count
04:18 -!- rafspiny [~raffaele@a120210.upc-a.chello.nl] has quit [Read error: Connection reset by peer]
04:19 -!- rafspiny [~raffaele@a120210.upc-a.chello.nl] has joined #hplusroadmap
04:22 < hprmbridge> kanzure> dorsolateral prefrontal cortex neuronal implants from smarter person.
04:29 < fenn> and where do you get those
04:30 < fenn> no man, you can't just chop some brains up and shove em in
04:30 < fenn> that's not how any of this works
04:31 < fenn> it's kind of frustrating how easy it is to glue together artificial neural networks and it just works
04:34 -!- A_Dragon [A_D@libera/staff/dragon] has quit [Quit: ZNC - https://znc.in]
04:35 -!- A_Dragon [A_D@libera/staff/dragon] has joined #hplusroadmap
04:35 -!- Netsplit *.net <-> *.split quits: drmeister, strages, WizJin_, Malvolio, helleshin, Gooberpatrol_66
04:38 -!- A_Dragon is now known as gAy_Dragon
04:39 -!- strages [sid11297@id-11297.helmsley.irccloud.com] has joined #hplusroadmap
04:40 -!- hellleshin [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has joined #hplusroadmap
04:40 -!- WizJin__ [~Wizzy@2402:a00:184:8354:e543:2733:1fa1:9bc5] has joined #hplusroadmap
04:40 -!- Gooberpatrol_66 [~Gooberpat@user/gooberpatrol66] has joined #hplusroadmap
04:40 -!- drmeister [sid45147@id-45147.ilkley.irccloud.com] has joined #hplusroadmap
04:40 -!- Gooberpatrol_66 [~Gooberpat@user/gooberpatrol66] has quit [Max SendQ exceeded]
04:40 -!- Gooberpatrol_66 [~Gooberpat@user/gooberpatrol66] has joined #hplusroadmap
04:41 < hprmbridge> kanzure> you can actually do this and people have.
04:41 < hprmbridge> kanzure> and mice show improved learning as a result.
04:42 < hprmbridge> kanzure> technically this would be a cell therapy.... well, surgically administered.
04:44 < fenn> i saw it on starship troopers
05:01 -!- andytoshi [~apoelstra@user/andytoshi] has quit [Server closed connection]
05:01 -!- andytoshi [~apoelstra@user/andytoshi] has joined #hplusroadmap
06:21 < hprmbridge> kanzure> chemical activators of telomerase can reach most cells https://x.com/BWalkerTTAGGG/status/1969028417749209231
06:21 < hprmbridge> kanzure> https://pmc.ncbi.nlm.nih.gov/articles/PMC8369046/
06:29 < kanzure> hmph
06:50 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=5bf12e36 Bryan Bishop: add hedonistic imperative david pearce germline recommendations >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
07:04 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=628cad75 Bryan Bishop: add selegiline-related longevity genetics, fix table of contents >> 
07:47 -!- TMM [hp@amanda.tmm.cx] has quit [Quit: https://quassel-irc.org - Chat comfortably. Anywhere.]
07:48 -!- TMM [hp@amanda.tmm.cx] has joined #hplusroadmap
08:04 < hprmbridge> jerlendds> https://www.alterego.io/
08:37 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=50d63bd8 Bryan Bishop: huh, infrared vision is pretty hard >> 
08:37 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=ec8fb474 Bryan Bishop: reorganize >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
08:38 < kanzure> jerlendds: what is their sensor technology? the webpage doesn't say, which is why i didn't link it earlier :\
08:47 <+gnusha_> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=119152b0 Bryan Bishop: more reorg >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
08:53 -!- L29Ah [~L29Ah@wikipedia/L29Ah] has left #hplusroadmap []
09:13 -!- L29Ah [~L29Ah@wikipedia/L29Ah] has joined #hplusroadmap
09:21 < hprmbridge> jerlendds> @kanzure
09:21 < hprmbridge> jerlendds> > In creating a subvocal speech recognition system, the signals of interest are the in-
09:21 < hprmbridge> jerlendds> > dividual neurological activations of internal speech articulators. As a non-invasive
09:21 < hprmbridge> jerlendds> > device, there is no way for AlterEgo to directly record these signals. Instead, they
09:21 < hprmbridge> jerlendds> > must be collected externally, far from the actual source. The best method for doing
09:21 < hprmbridge> jerlendds> > this is with electrodes that can pick up on subtle disruptions in the electric field
09:21 < hprmbridge> jerlendds> > surrounding speech articulators. With these electrodes placed on the face and neck
09:21 < hprmbridge> jerlendds> > regions, as in Figure 2-1, an aggregation of the desired neuromuscular signals is ap-
09:21 < hprmbridge> jerlendds> > parent during internal articulation.
09:21 < hprmbridge> jerlendds> You can find his thesis here: https://dspace.mit.edu/bitstream/handle/1721.1/123121/1128187233-MIT.pdf?sequence=1
09:30 -!- gnusha [~gnusha@user/gnusha] has joined #hplusroadmap
09:30 -!- Topic for #hplusroadmap: biohacking, nootropics, transhumanism, open hardware | this channel is LOGGED: http://gnusha.org/logs | https://diyhpl.us/wiki | not quite sponsored by george church | banned by the MIT media lab and the FDA
09:30 -!- Topic set by fenn [~fenn@user/fenn] [Fri Mar 25 06:29:19 2022]
09:30 -!- Irssi: #hplusroadmap: Total of 59 nicks [1 ops, 0 halfops, 0 voices, 58 normal]
09:30 -!- mode/#hplusroadmap [+v gnusha] by ChanServ
09:30 -!- Channel #hplusroadmap created Wed May 19 06:51:36 2021
09:30 -!- kanzure [~kanzure@user/kanzure] has joined #hplusroadmap
09:30 -!- fenn [~fenn@user/fenn] has joined #hplusroadmap
09:30 -!- jrayhawk [~jrayhawk@user/jrayhawk] has joined #hplusroadmap
09:32 -!- Irssi: Join to #hplusroadmap was synced in 144 secs
09:44 < hprmbridge> kanzure> Thank you @jerlendds . CTC+ CNN + an autoencoder seems reasonable... 100 wpm from only 8 electrodes for subvocalization? but only 20 word vocabulary?
09:49 < hprmbridge> jerlendds> Yeah the dataset feels like its lacking imo however I plan to start researching and experimenting in this area as alterego is taking far too long to commercialize for my tastes. I want one now aha, Im currently looking into constructing/adapting something such as https://github.com/pieeg-club/EEGwithRaspberryPI for the purposes of subvocal speech detection like alterego. Ill probably use/create
09:49 < hprmbridge> jerlendds> the same dataset they started with and afterwards scale the dataset up to see what happens
09:49 < hprmbridge> kanzure> https://github.com/facebookresearch/emg2qwerty the facebook wrist EMG sensor data set is public. i think they are requiring consumers to purchase the glasses to get the EMG wristband. there's also https://mindrove.com/product/emg-armband/ but not sure how the facebook hardware compares.
09:50 < hprmbridge> jerlendds> This is interesting, I've heard about this research but I didnt realize the repos were public, thanks for the links
09:51 < hprmbridge> kanzure> they should do neurofeedback to train the classifier and the user at the same time.
09:52 < hprmbridge> kanzure> "Inner speech in motor cortex and implications for speech neuroprostheses" https://www.cell.com/cell/fulltext/S0092-8674(25)00681-6 targeting motor cortex.
09:52 < hprmbridge> kanzure>
09:52 < hprmbridge> kanzure> "An instantaneous voice-synthesis neuroprosthesis" https://www.nature.com/articles/s41586-025-09127-3 this implant targeted ventral precentral gyrus, as discussed in here 2 months ago
09:54 -!- catalase [~catalase@user/catalase] has quit [Remote host closed the connection]
09:54 < hprmbridge> kanzure> alterego seems to be doing an AI pendant thing instead of directly commercializing subvocalization hardware.
09:55 < hprmbridge> kanzure> but what do I know, neuralink has 600 employees and only ten customers.
09:55 -!- catalase [~catalase@user/catalase] has joined #hplusroadmap
09:57 -!- catalase [~catalase@user/catalase] has quit [Remote host closed the connection]
09:58 -!- catalase [~catalase@user/catalase] has joined #hplusroadmap
10:12 < hprmbridge> kanzure> hm someone should do directed selection for embryos that can implant in foreign uterus. a more virulent embryo.
10:17 < hprmbridge> kanzure> "Classic interspecific chimera studies show that survival correlates with having extraembryonic/trophoblast tissues of the maternal uterine species."
10:23 -!- L29Ah [~L29Ah@wikipedia/L29Ah] has left #hplusroadmap []
10:29 < hprmbridge> kanzure> "Using the amniotic cavity of the developing chick embryo for the in vivo culture of early-stage mammalian embryos" https://pubmed.ncbi.nlm.nih.gov/2622823/
10:34 < hprmbridge> kanzure> in ovo mammalian embryo incubation also works for caprine, goat and cow embryos (live births after transplanting to normal uterus after a few days) https://link.springer.com/article/10.1007/BF01404647
10:35 < hprmbridge> kanzure> "Use of in ovo chorioallantoic membrane engraftment to culture testes from neonatal mice"  https://pmc.ncbi.nlm.nih.gov/articles/PMC4170091/
10:53 -!- L29Ah [~L29Ah@wikipedia/L29Ah] has joined #hplusroadmap
11:25 -!- TC [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has joined #hplusroadmap
11:27 -!- catalase [~catalase@user/catalase] has quit [Killed (NickServ (GHOST command used by catalase1))]
11:27 -!- catalase1 [~catalase@user/catalase] has joined #hplusroadmap
11:31 -!- nmz787__ [~nmz787@user/nmz787] has joined #hplusroadmap
11:34 -!- Netsplit *.net <-> *.split quits: hellleshin, drmeister, WizJin__, nmz787
11:34 -!- TC is now known as 029AAEHXP
11:34 -!- TC [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has joined #hplusroadmap
11:34 -!- Netsplit over, joins: WizJin__, drmeister
11:35 -!- catalase [~catalase@user/catalase] has joined #hplusroadmap
11:36 -!- catalase1 [~catalase@user/catalase] has quit [Quit: Ping timeout (120 seconds)]
11:37 -!- jrayhawk [~jrayhawk@user/jrayhawk] has quit [Ping timeout: 256 seconds]
11:37 -!- jrayhawk [~jrayhawk@user/jrayhawk] has joined #hplusroadmap
11:37 -!- 029AAEHXP [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has quit [Ping timeout: 256 seconds]
11:37 -!- nmz787_ [~nmz787@user/nmz787] has quit [Ping timeout: 256 seconds]
11:38 -!- nmz787 [~nmz787@user/nmz787] has joined #hplusroadmap
12:13 -!- dustinm [~dustinm@static.38.6.217.95.clients.your-server.de] has quit [Ping timeout: 258 seconds]
12:14 -!- rafspiny [~raffaele@a120210.upc-a.chello.nl] has quit [Read error: Connection reset by peer]
12:14 -!- rafspiny [~raffaele@a120210.upc-a.chello.nl] has joined #hplusroadmap
12:15 -!- dustinm [~dustinm@static.38.6.217.95.clients.your-server.de] has joined #hplusroadmap
12:39 < kanzure> "Baltimore also helped Paul Berg and Maxine Singer to organize the Asilomar Conference on Recombinant DNA, held in February 1975. The conference discussed possible dangers of new biotechnology, drew up voluntary safety guidelines, and issued a call for an ongoing moratorium on certain types of experiments and review of possible experiments, which has been institutionalized by recombinant DNA ...
12:39 < kanzure> ...advisory committees established at essentially all US academic institutions conducting molecular biology research.[5] Baltimore was well aware of the importance of the changes occurring in the laboratory: "The whole Asilomar process opened up to the world that modern biology had new powers that you had never conceived of before."[10]: 111"
12:45 < kanzure> he set back science a lot, and for his efforts he was awarded a nobel prize that same year.
12:53 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=23f9ece8 Bryan Bishop: add a note about human cloning >> http://diyhpl.us/diyhpluswiki/diabetes/
13:12 < kanzure> wait, do we do mouse GWAS or not?
13:26 < hprmbridge> .monokhrome> what are meta's new smart glasses meant to do? I don't understand what the point is
13:29 < L29Ah> personal computer
13:29 < hprmbridge> .monokhrome> I was promised this technology a decade ago with google glasses
13:29 < L29Ah> i didn't read further after i saw the screen resolution and the fact it is a self-contained unit
13:30 < L29Ah> xreal air + SBC/laptop seems a better bet
13:31 < hprmbridge> .monokhrome> it just sounds like it's cameras and a microphone, you can already get that with your smartphone
13:31 < L29Ah> smartphone is awkward to use
13:34 < L29Ah> i want to be plugged in 24/7, smartphone is a bit more comfy than a laptop for cases when you're going somewhere (unless you do the RMS mod that is questionable by itself), but still meh, i'd like to be able to do hiking and cycling ideally among other things
13:35 < hprmbridge> .monokhrome> if you want to go around recording things with a camera, you can just put the phone in your shirt pocket
13:35 < L29Ah> although looking at my progress so far i might actually do the RMS mod instead, seems more compatible with Framework 13 than with Thinkpad X*
13:35 < L29Ah> i don't, i have GoPro and a helmet, or can print a chest mount, for that
13:36 < L29Ah> i want a display and a decent input device
13:36 < L29Ah> glasses are supposed to do the first part
13:37 < hprmbridge> .monokhrome> i think meta's glasses just have a small led display for printing out the time and stuff, not a real AR one
13:37 < hprmbridge> .monokhrome> It just seems like zuckerberg's got all the money in the world, but everything he announces, eg the metaverse is somehow lame
13:53 -!- streety_ [~streety@176-58-127-93.ip.linodeusercontent.com] has quit [Server closed connection]
13:54 -!- streety [~streety@176-58-127-93.ip.linodeusercontent.com] has joined #hplusroadmap
13:58 < L29Ah> https://luminaprobiotic.com/cdn/shop/files/Untitled_design.png?v=1749001360&width=1024 ok the ingredients list basically says how to store it
15:00 -!- Gooberpatrol_66 [~Gooberpat@user/gooberpatrol66] has quit [Quit: Konversation terminated!]
15:00 < L29Ah> https://tinystash.undef.im/il/1DFGaJKUPDvJpzAHLoiUME4hSDm1BSJHVtRRmGERtQUHRGPEg4TfsC1sEMj9XFKdp56C5WV1vdje4fJJwaZTZTy.jxl RMS mode on
15:01 -!- Gooberpatrol66 [~Gooberpat@user/gooberpatrol66] has joined #hplusroadmap
15:19 -!- Gooberpatrol66 [~Gooberpat@user/gooberpatrol66] has quit [Quit: Konversation terminated!]
15:31 < hprmbridge> kanzure> "injection of siRNA targeting the SERPINA6 gene on day 17 of chicken egg incubation caused  a notably positive impact on embryo development characteristics when compared to the other treatments, including increased embryo weight and length, improved yolk and albumin consumption, and a significant increase in shell thickness (P ≤ 0.05)." https://www.researchsquare.com/article/rs-7087632/v1 from
15:31 < hprmbridge> kanzure> university of anbar, iraq 2025
15:33 < hprmbridge> kanzure> just throw some siRNA in there i guess?
15:37 -!- Gooberpatrol66 [~Gooberpat@user/gooberpatrol66] has joined #hplusroadmap
15:51 -!- Gooberpatrol66 [~Gooberpat@user/gooberpatrol66] has quit [Quit: Konversation terminated!]
16:08 < kanzure> "Apple mouse has one button, why can't this spaceship?" designer brain goes brrr
16:19 < kanzure> https://www.gutenberg.org/files/27683/27683-h/27683-h.htm#chap11 section "before the soul dawn" where hellen keller describes her "before time".
16:24 < kanzure> .wik tadoma
16:24 < saxo> "Short description is different from Wikidata Articles needing additional references from February 2023 All articles needing additional references" - https://en.wikipedia.org/wiki/Tadoma
16:24 < kanzure> hmph
16:26 < kanzure> "tadoma is sometimes referred to as tactile lipreading, as the listener feels the movement of the lips, the vibrations of the vocal cords, expansion of the cheeks and the warm air produced by nasal phonemes such as 'N' and 'M'."
17:01 -!- Malvolio [~Malvolio@idlerpg/player/Malvolio] has joined #hplusroadmap
17:19 -!- stipa_ [~stipa@user/stipa] has joined #hplusroadmap
17:21 -!- stipa [~stipa@user/stipa] has quit [Ping timeout: 244 seconds]
17:21 -!- stipa_ is now known as stipa
17:24 < hprmbridge> kanzure> the 64th annual conference of the "American Society of ExtraCorporeal Technology" will be hosted in austin in 2026
17:28 < hprmbridge> kanzure> https://ject.edpsciences.org/
17:33 < hprmbridge> kanzure> "patient received ECMO support for a total of 259 days" https://ject.edpsciences.org/articles/ject/full_html/2025/01/ject240048/ject240048.html that's close to the ~300  day record.
18:01 < hprmbridge> kanzure> "Fusogen-induced recovery of spinal cord function and morphology after complete transection" https://www.biorxiv.org/content/10.1101/2025.09.13.674972v1 just use PEG as a fusogen. alright!
18:04 < hprmbridge> kanzure> from hydraDAO. I am not sure that transection followed by immediate fusion was unexpected to work? I thought it was well known in neurosurgery that time is of the essence after transection of a nerve.
18:24 < hprmbridge> kanzure> rfdiffusion3 https://www.biorxiv.org/content/10.1101/2025.09.18.676967v1
18:26 < hprmbridge> kanzure> "Epinoma  is commercializing a methylation-preserving nucleic acid amplification chemistry"
18:56 -!- catalase [~catalase@user/catalase] has quit [Remote host closed the connection]
18:57 -!- catalase [~catalase@user/catalase] has joined #hplusroadmap
19:07 < hprmbridge> Eli> After watching RFK jr put methylene blue into his drink, I kind of thought it might just be another risky research chmeical with low n-value human data. I've been doing research, and it actually might be legit. They just wrapped up a phase III on a form of MB for alzheimers and it might get approved. It's already approved for other stuff, like malaria and shock. And it's the oldest synthetic
19:07 < hprmbridge> Eli> medical drug that we have. We used to give it to huge numbers of people in the military. Based on it's MOA, it actually seems like a possible legit nootropic with low-side effect risk. Kind of curious to try it.
19:08 < hprmbridge> Eli> MOA is basically increase in ATP generation due to speeding up the ETC. And, it preferentially accumulates in the CNS, which requires huge amounts of ATP.
19:14 < hprmbridge> Eli> I found this paper from 1891 by paul ehrlich. This is the first paper on a successful synthetic drug in humans. https://cdn.discordapp.com/attachments/1064664282450628710/1418782441669394562/Screenshot_2025-09-19_at_12.37.43_PM.png?ex=68cf5f9d&is=68ce0e1d&hm=9ad3a814ace9bfd229aebf88035b252d2eaac6e6b1ba38fe13c888c1843b731d&
19:17 < L29Ah> methylene blue has undesirable side effects when taken internally iirc
19:18 < L29Ah> but it could be a working anti-aging drug for skin 
19:18 < L29Ah> from my notes: https://pubmed.ncbi.nlm.nih.gov/2680144/ https://pubs.rsc.org/en/content/articlehtml/2016/cp/c6cp02529c
19:18 < L29Ah> MuaddibLLM: what do you think?
19:19 < MuaddibLLM> diving into your methylene blue papers and checking current data...
19:19 < MuaddibLLM> checking LMTM Alzheimer's trials and topical safety data...
19:20 < MuaddibLLM> Methylene blue's singlet oxygen generation could theoretically benefit skin aging through antimicrobial/cellular effects, but the same DNA-damaging mechanism from your 1989 paper https://pubmed.ncbi.nlm.nih.gov/2680144/ raises concerns - plus systemic use has serious risks (serotonin syndrome, G6PD contraindication) per https://www.ncbi.nlm.nih.gov/books/NBK557593/ and the Alzheimer's LMTM trials have been disappointing 
19:20 < MuaddibLLM> https://www.alzheimersresearchuk.org/news/lmtm/, so topical might be the only viable route if at all.
19:26 < hprmbridge> Eli> Mauddibllm you’re wrong and AI won’t replace researchers yet
19:26 -!- TC [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has quit [Read error: Connection reset by peer]
19:31 < L29Ah> https://tinystash.undef.im/il/4v1LyFhY19s8GkRsxRUeawmREry4ksdgE7hVTuoFPDyf3Yk94AN8j7BTh2q2eZ78Hv3F46b29BnJiYsx9nE7nCw7.png
19:33 < hprmbridge> Eli> Mauddibllm you can’t replace meme researchers yet either
20:06 <+gnusha> https://secure.diyhpl.us/cgit/diyhpluswiki/commit/?id=366799af Bryan Bishop: improve eye color genetics, immune system, autoimmune disease protection, etc >> http://diyhpl.us/diyhpluswiki/genetic-modifications/
20:35 < fenn> Centella asiatica extract prevents telomere shortening *and* decreases telomerase activty? huh? aren't those opposite effects? https://pmc.ncbi.nlm.nih.gov/articles/PMC8369046/
20:49 -!- helleshin [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has joined #hplusroadmap
20:50 < hprmbridge> .monokhrome> how can it prevent telomere shortening? isn't that an inherent property of dna polymerase?
20:56 < fenn> one possible way is to add more telomere material with telomerase... but that process is decreased, at least according to this paper
21:00 < hprmbridge> .monokhrome> i meant besides direct telomere lengthening with telomerase
21:02 -!- superkuh [~superkuh@user/superkuh] has quit [Ping timeout: 258 seconds]
21:09 < fenn> it could reduce cell division rate
21:10 < hprmbridge> .monokhrome> That sounds like it could have all sorts of unpleasant side effects.
21:11 < fenn> that's what happens when your telomeres get too short
21:12 < fenn> MuaddibLLM: read this https://pmc.ncbi.nlm.nih.gov/articles/PMC8369046/ and note whether they controlled for cell division cycle count / rate
21:13 < MuaddibLLM> diving into that centella paper to see if they actually controlled for cell proliferation rates...
21:13 < fenn> 2mg/ml extract seems to push out the kaplan death curve
21:13 < MuaddibLLM> Looking at this Centella asiatica paper, they did NOT control for cell division rate/cycle count - major oversight that invalidates their core findings. They measured telomere length at passages 13/23/33/43 but only normalized to 36B4 reference gene, with zero mention of proliferation assays, population doubling levels, or cell cycle corrections https://pmc.ncbi.nlm.nih.gov/articles/PMC8369046/ - the paradox of "preserved 
21:13 < MuaddibLLM> telomeres + decreased telomerase" screams reduced division rate, which they completely missed.
21:14 < fenn> MuaddibLLM: wouldn't measuring at a specific passage number control for cycle count?
21:14 < MuaddibLLM> Not really - “passage 20 vs 40” is a blunt instrument because split ratios, plating density, growth rate, senescence and cell loss all change cumulative population doublings, so two cultures at the same passage can have very different division counts; you’d control with recorded CumPDL (or CPD), fixed split ratios and seeding numbers, growth curves/doubling time, and proliferation markers (EdU/BrdU, Ki‑67), ideally 
21:14 < MuaddibLLM> alongside cell cycle staging and senescence assays (SA‑β‑gal, p16/p21).
21:18 -!- superkuh [~superkuh@user/superkuh] has joined #hplusroadmap
21:20 < fenn> their female drosophila seem very unhealthy
21:21 -!- hellleshin [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has joined #hplusroadmap
21:24 -!- helleshin [~talinck@76-230-66-1.lightspeed.cntmoh.sbcglobal.net] has quit [Ping timeout: 256 seconds]
21:25 -!- stipa [~stipa@user/stipa] has quit [Ping timeout: 256 seconds]
21:25 -!- stipa [~stipa@user/stipa] has joined #hplusroadmap
21:35 -!- Croran_ is now known as Croran
21:40 < hprmbridge> .monokhrome> Elon Musk doesn't approve of life extension: https://www.businessinsider.com/elon-musk-interview-axel-springer-tesla-war-in-ukraine-2022-3
21:40 < hprmbridge> .monokhrome> ```it would cause asphyxiation of society because the truth is, most people don't change their mind. They just die. So if they don't die, we will be stuck with old ideas and society wouldn't advance. ```
21:46 -!- TMM [hp@amanda.tmm.cx] has quit [Quit: https://quassel-irc.org - Chat comfortably. Anywhere.]
21:46 -!- TMM [hp@amanda.tmm.cx] has joined #hplusroadmap
21:51 -!- Gooberpatrol66 [~Gooberpat@user/gooberpatrol66] has joined #hplusroadmap
22:05 < hprmbridge> Eli> Our brains suck when we get old. Need to regrow them somehow.
--- Log closed Sat Sep 20 00:00:45 2025