# Key Points from IRC Log (Feb 19, 2009)

* A discussion about SKDB (skill database) was mentioned, with reference to a wiki page at heybryan.org, suggesting ongoing development of an open hardware/skills database system.

* Kanzure shared information about a maskless photolithography system for synthesizing Ultra Large Scale DNA chips, capable of producing chips with up to 20 million pixels, demonstrating interest in DIY DNA synthesis technologies.

* A detailed pathway for synthesizing dopamine in cells was posted to the DIYbio mailing list, generating immediate interest from prominent biohackers including Andrew Hessel and Tito.

* A novel microwave-based method for improving E. coli transformation efficiency was discussed as a low-cost alternative to electroporation, specifically noting it could be useful for labs without expensive equipment.

* The group discussed "The Farmery," a concept for vertical farming using stacked shipping containers with attached greenhouses for hydroponic crop cultivation and mushroom growing, with nutrients sourced from composted local food waste.

* Members discussed DIY biology equipment, including the possibility of creating a $25 thermocycler (PCR machine) at a Fab Lab and using UV disinfectors as DNA illuminators for gel electrophoresis.

* Kanzure mentioned an upcoming call with Carolina Biological to discuss connecting their API to XML biology protocols for automatically purchasing parts for DIYbio kits.

* A graph showing the exponential growth of interest/activity in DIYbio was shared, illustrating the rapid expansion of the community and its "holy crap" moments.