# Key Points from #hplusroadmap IRC Discussion

• Researchers shared techniques for low-cost microfluidics fabrication, including micromolding in capillaries and capillary force lithography, with particular interest in accessible methods using common materials like nail polish and Sharpie markers as alternatives to expensive conventional lithography.

• The group extensively discussed DNA/gene synthesis technologies, focusing on PCR-based methods (especially overlap extension PCR) and exploring alternatives to traditional phosphoramidite chemistry, with detailed analysis of error sources in oligonucleotide synthesis.

• Participants debated various approaches to DNA synthesis including droplet-based methods, DLP mirror arrays, and LCD-based systems for maskless array synthesis, sharing resources like a wiki on DNA synthesizer technology (http://heybryan.org/mediawiki/index.php/DNA_synthesizer).

• The conversation included technical analysis of forces relevant at the micron-to-millimeter scale (gravitational, electrostatic, magnetic, capillary) with reference to Whitesides' research, and discussion of novel materials including shear-thickening fluids, quantum tunneling composites, and ferrofluids.

• Space technology was discussed through Keith Henson's work on space power satellites and the "Space Renaissance" initiative, with resources shared about achieving "$100/kg" space launch costs.