# Summary of #hplusroadmap IRC Log (March 23, 2009)

• Discussion of DNA assembly techniques, specifically Polymerase Cycling Assembly (PCA), where 40-nucleotide oligonucleotides are joined into longer strands, with reference to research on "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides."

• Kanzure shared a resource on oligonucleotide errors (http://heybryan.org/books/papers/oligo-errors/) and proposed creating a "rubber band ball of DNA" ligated from different organisms as a demonstration project for DIYbio enthusiasts.

• A physics phenomenon was discussed where peeling adhesive tape in a vacuum produces X-rays, with reference to the paper "Correlation between nanosecond X-ray flashes and stick-slip friction in peeling tape" published in Nature, with speculation about practical applications.

• Gene shared an article about historical Soviet "water integrators" - analog computing devices that used fluid dynamics to solve differential equations.

• Discussion of image processing techniques using ImageJ software for tracking objects in video, with applications for motion analysis and potential use in automated targeting systems.

• Conversation about computer vision applications, including using OpenCV for facial recognition and how such systems might interface with servomotors for practical applications like security systems.

• Reference to PMMA (plexiglass) as an effective photoresist material for X-ray lithography (LIGA) techniques.