# Key Points from #hplusroadmap IRC Discussion

• Researchers explored novel cell transfection methods including photoporation with violet LEDs (405nm), plasmid-decorated AFM probes for single-cell delivery, and carbon nanotube "spearing" techniques for introducing molecules into mammalian cells.

• A scalable DNA synthesis approach using LEDs and capillary tubes was discussed, capable of producing 160 nucleotides in 16 hours, potentially offering much lower-cost alternatives to commercial PCR primers.

• The group examined Atomic Force Microscopy (AFM) applications for biological visualization and manipulation, including direct observation of DNA translocation by enzymes and the possibility of using AFM for large genome synthesis through direct placement of DNA strands.

• Participants debated the feasibility of fluidic replicators and processors, calculating that an 8086-level processor could theoretically fit in a 2.5cm cube using shrinky dink microfluidics, though materials challenges were identified as significant obstacles.

• Various designs for open-source PCR thermocyclers were compared, including different heating approaches (aluminum blocks, peltier devices, water baths) and capillary tube systems with light bulbs similar to the Idaho Scientific RapidCycler.

• The discussion covered the integration of microfluidics with laser nanosurgery techniques for gene transfer into C. elegans, demonstrating interest in combining multiple technologies for advanced biological manipulation.

• Technical considerations about thermal properties of lab materials were shared, including how polypropylene and polyethylene tubes expand at high temperatures to create better thermal contact with container walls.