# Key Points from #hplusroadmap IRC Discussion

• Participants discussed cutting-edge DNA sequencing technology, specifically Single Molecule Real Time (SMRT) Sequencing using zero-mode waveguides (ZMW), with interest in potentially creating DIY versions before commercial release in 2010-2011.

• The group explored DNA polymerase mechanics in detail, examining how conformational changes and binding pocket structures ensure accurate base pair matching during DNA replication.

• Discussion included analysis of the Mycoplasma genitalium genome (580,073 base pairs) as a potential target for ZMW sequencing, considering the technical limitations of the method (1,000 holes, 10 bp/sec, 1.5k bp max per hole).

• Participants shared technical resources, including a collection of biochemistry papers, and discussed challenges of managing large scientific literature libraries.

• The conversation explored speculative biotechnology concepts, including the possibility of creating hybrid organic/inorganic structures by folding proteins around nanowire scaffolds to create functional sensors.

• Various photosensitive proteins were discussed, including Vivid, White Collar-1, ZEITLUPE, and others categorized into three types: cryptochromes, LOV (Light, Oxygen and Voltage), and BLUF (Blue Light sensing Using FAD) proteins.

• The group referenced freedomofscience.org as a project developing a wiki-based community annotation system for reviewing scientific licenses.