# Key Technical and Conceptual Points from #hplusroadmap IRC Logs

• DIY microscopy was explored with specific focus on two-photon microscope designs, with resources from Parker Lab at UCI being archived for community reference, demonstrating the channel's commitment to open-source scientific instrumentation.

• A comprehensive taxonomy of manufacturing processes was created and visualized using graphviz, showing the community's interest in systematizing and mapping technological capabilities through cladogram-style diagrams.

• Detailed technical discussions on DIY DNA synthesis centered on photolabile protecting groups like NPPOC-Cl and phosphoramidites, with participants debating chemical approaches and noting the high cost of commercial reagents ($480/g for NPPOC-dC(ib) Amidite).

• Various separation techniques for nucleosides were evaluated, including gel electrophoresis, thin-layer chromatography (TLC), and paper chromatography, with practical DIY approaches such as making TLC plates from glass slides and silica gel packets.

• The channel demonstrated integration of diverse technologies by discussing SKDB (standard parts library) and its connection to symbolic mathematics through sympy for handling units in equations, alongside mentions of RepRap 3D printing technology.

• Resources related to "adesign" were shared through data archives, though participants noted limitations in the available data, reflecting the community's critical approach to evaluating shared resources.