# Key Points from #hplusroadmap IRC Discussion

• A DIY microfluidic protein purification device using differential centrifugation on a CD platform was discussed, capable of processing 4-11 microliters of samples including GFP variants, with potential applications for home-based purification of proteins like Taq polymerase.

• One participant is creating bipolar junction transistors (BJTs) in a garage setup, using a modified microwave as a crucible to refine silicon from sand, demonstrating advanced DIY semiconductor fabrication techniques.

• Research from Hong Tang published in Nature Photonics was shared about creating controllable repulsive forces by splitting infrared light into two beams traveling through different lengths of silicon nanowire.

• The group discussed DIY welding techniques, including a homemade welder built from microwave transformers capable of delivering 30V at 150A (3kW), comparing its capabilities to professional equipment.

• Participants debated climate change perspectives, with some viewing it as potentially causing mass extinctions while others characterized it as an "overblown nuisance."

• Resources on biosafety were shared, including the "Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition" and maps of high containment labs, with related discussions about safety standards for home biology labs.

• The Oregon State University Open Source Lab was highlighted as a potential hosting resource for future projects, noting its support of numerous open source initiatives with over a million dollars in annual donations.