# Key Points from #hplusroadmap IRC Log (August 4, 2009)

• Developers were building SKDB, described as "apt-get but for hardware," a package management system for physical objects that could generate instructions for building devices like RepRap 3D printers.

• The team was implementing YAML-based metadata systems for hardware components, working through technical issues with tag handling, class loading, and package management for physical parts.

• Microfluidics technology was a focus, with participants sharing and uploading files titled "MicrofluidicsHistoryTheoryandApplications.zip," indicating interest in DIY microfluidics development.

• Neural interface technologies were discussed, including a shared document on manufacturing microelectrode arrays (MEAs) using wire EDM technology, and Topographica (topographica.org), a neural map simulator for computational neuroscience.

• A detailed technical conversation covered PCR techniques for cheese identification, including DNA isolation methods, housekeeping genes, and the need for additional analysis like gel electrophoresis and sequencing.

• Participants discussed advanced biomedical topics including RNA synthesis methods, ribosome display, in vitro protein synthesis, and siRNA for gene silencing.

• Research on repetitive transcranial magnetic stimulation (rTMS) was mentioned, specifically its ability to induce short-term reorganization of the human motor cortex and potential effects on fetuses.

• A researcher noted work with mesenchymal stem cells (MSCs) in culture, with specific discussion about VEGF (Vascular Endothelial Growth Factor) isoforms (121, 165, or 189 amino acids) used in this research.