# Key Technical and Conceptual Points from #hplusroadmap Discussions

• Participants explored the physiological effects of ketosis, describing it as "fascinatingly transformational" with widespread changes in expressed genetics and bodily systems, while discussing mitigation strategies for side effects through probiotics like kimchi and yogurt.

• A significant technical project involving a tongue-based electrode array with 256 bidirectional electrodes was shared, with comparative discussion of microcontroller options (Arduino versus AVR chips) for such bioelectronic interfaces.

• Detailed molecular biology discussions covered plasmid cloning challenges, restriction enzyme limitations, and methods for oligonucleotide library creation, including a proposed system using DNA templates on beads with polymerase and nicking enzymes to generate 6-mer fragments.

• Researchers debated microfluidic approaches for DNA synthesis, suggesting water-in-oil droplets as an alternative to multiple chambers due to valving constraints, while expressing disappointment with current automated synthesis speeds (only 10.5 base pairs per hour).

• Neuroscience research was shared regarding mania, including papers on "Mania-like behavior induced by disruption of CLOCK" and "Transcranial magnetic stimulation-induced switch into mania," alongside research from the Salk Institute about Extremely Long-Lived Proteins (ELLPs).

• The group engaged in philosophical discussion about programming literacy in society, contemplating the implications of a potential "post-literate" programming environment and its effects on technological development.