# Key Ideas from #hplusroadmap IRC Discussions

• Participants explored developing an affordable open-source DNA synthesizer (targeting $5-10K vs commercial $100K+ machines), discussing microfluidics approaches, valve technologies, and comparing traditional phosphoramidite chemistry with novel methods using oligonucleotide libraries with sticky ends.

• A novel DNA synthesis method was proposed using droplet microfluidics with reusable 6-mer oligonucleotides featuring 3-nucleotide overhangs, with detailed discussions about whether to use ligase or polymerase for connecting fragments.

• DIY biology safety concerns were discussed, noting how law enforcement often overreacts to basic laboratory equipment despite the ubiquity of microorganisms like E. coli in everyday environments.

• The group shared information about open-source biology tools including OpenPCR and the POSAM/POGO project (Piezoelectric Oligonucleotide Synthesizer And Microarrayer), alongside discussions about breaking the "NEB/Fischer oligopoly" on research enzymes.

• A biohacking project to genetically engineer bacteria to produce linalool (a fragrant compound) was discussed, including technical aspects of the mevalonate pathway and protein scaffolding to increase production efficiency.

• Participants critically analyzed viral scientific claims, including a video purporting to show how to make Prozac in yogurt, with members noting the chemical implausibility of bacteria adding CF3 groups.

• Resources were shared including a collection of scientific papers at diyhpl.us/~bryan/papers2/ covering microfluidics, longevity research, and polymerase technologies, as well as information about a microfluidics bootcamp at Princeton.